Abstract
The emergence of life requires the appropriate integration of protometabolisms, compartments, and protogenomes from an intricate interplay of non-living constituents. Unveiling the mechanism of how life-related building blocks are logically integrated towards life from a high diversity of inanimate matter is an ongoing challenging task of current science. Various compartments such as lipid vesicles and coacervates have been proposed as possible microcontainers to hold prebiotic genetic materials and metabolic reactions for the construction of integrated systems. However, the spontaneous assembly of these compartments allows no selective and logical integration from a high diversity of inanimate matter, thus making the appropriate integration of the inanimate towards membranous life more a coincident and low probability event. Herein, we show that the assembly of colloidal particles with coacervate-forming molecules provides a combinatorial approach for the regularization of matter of chaos towards protocells with cellular hallmarks of size uniformity, logical integration, and unilamellar membransation. Monodisperse coacervate droplets coated by colloidal particles are assembled through hydrodynamic forcing-promoted coalescence. Using these coacervates as platform, a combinatorially integrative approach is developed to engineer the complexity of coacervates, from coacervate entities with programmable spatial loading to diverse interconnected coacervate consortia with collective morphology evolution. A fluidic unilamellar membrane is assembled on coacervate via freeze-thaw treatment of coacervates coated by liposome particles, including liposome particles with heterogenous lamellarity, resulting in coacervate-supported monodisperse giant unilamellar vesicles with gated permeability to polar molecules and remarkable structural and functional stability at extreme environments. This work provides an integrative approach to process crude building blocks towards disciplined and integrated cellular systems, which might have mediated the transition from the inanimate to life. This approach is promisingly utilized for high throughput screening of possible integrated form of primitive life from a high diversity of inanimate matter as well as on demand bulk-generation of monodisperse hierarchical microdroplets with flexibly integrated functions.
Competing Interest Statement
The authors have declared no competing interest.
