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Reduced binding and neutralization of infection- and vaccine-induced antibodies to the B.1.351 (South African) SARS-CoV-2 variant

View ORCID ProfileVenkata Viswanadh Edara, Carson Norwood, Katharine Floyd, Lilin Lai, View ORCID ProfileMeredith E. Davis-Gardner, William H. Hudson, Grace Mantus, Lindsay E. Nyhoff, Max W. Adelman, Rebecca Fineman, Shivan Patel, Rebecca Byram, Dumingu Nipuni Gomes, Garett Michael, Hayatu Abdullahi, Nour Beydoun, Bernadine Panganiban, Nina McNair, Kieffer Hellmeister, Jamila Pitts, Joy Winters, Jennifer Kleinhenz, Jacob Usher, James B. O’Keefe, View ORCID ProfileAnne Piantadosi, View ORCID ProfileJesse J. Waggoner, View ORCID ProfileAhmed Babiker, David S. Stephens, Evan J. Anderson, Srilatha Edupuganti, Nadine Rouphael, View ORCID ProfileRafi Ahmed, View ORCID ProfileJens Wrammert, View ORCID ProfileMehul S. Suthar
doi: https://doi.org/10.1101/2021.02.20.432046
Venkata Viswanadh Edara
1Center for Childhood Infections and Vaccines of Children’s Healthcare of Atlanta, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
2Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
7Yerkes National Primate Research Center, Atlanta, GA 30329, USA
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  • ORCID record for Venkata Viswanadh Edara
Carson Norwood
1Center for Childhood Infections and Vaccines of Children’s Healthcare of Atlanta, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
2Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Katharine Floyd
1Center for Childhood Infections and Vaccines of Children’s Healthcare of Atlanta, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
2Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
7Yerkes National Primate Research Center, Atlanta, GA 30329, USA
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Lilin Lai
1Center for Childhood Infections and Vaccines of Children’s Healthcare of Atlanta, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
2Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
7Yerkes National Primate Research Center, Atlanta, GA 30329, USA
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Meredith E. Davis-Gardner
1Center for Childhood Infections and Vaccines of Children’s Healthcare of Atlanta, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
2Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
7Yerkes National Primate Research Center, Atlanta, GA 30329, USA
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William H. Hudson
3Department of Microbiology and Immunology, Emory University, Atlanta, GA 30322, USA
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Grace Mantus
1Center for Childhood Infections and Vaccines of Children’s Healthcare of Atlanta, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
2Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Lindsay E. Nyhoff
1Center for Childhood Infections and Vaccines of Children’s Healthcare of Atlanta, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
2Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Max W. Adelman
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Rebecca Fineman
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Shivan Patel
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Rebecca Byram
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Dumingu Nipuni Gomes
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Garett Michael
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Hayatu Abdullahi
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Nour Beydoun
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Bernadine Panganiban
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Nina McNair
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Kieffer Hellmeister
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Jamila Pitts
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Joy Winters
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Jennifer Kleinhenz
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Jacob Usher
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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James B. O’Keefe
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Anne Piantadosi
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
6Department of Pathology and Laboratory Medicine, Emory University School of Medicine Atlanta, Georgia, USA
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Jesse J. Waggoner
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Ahmed Babiker
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
6Department of Pathology and Laboratory Medicine, Emory University School of Medicine Atlanta, Georgia, USA
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David S. Stephens
2Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
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Evan J. Anderson
1Center for Childhood Infections and Vaccines of Children’s Healthcare of Atlanta, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
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Srilatha Edupuganti
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
5Hope Clinic of Emory Vaccine Center, Emory University, Decatur, GA 30030, USA
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Nadine Rouphael
4Department of Medicine, Emory University School of Medicine, Atlanta, GA 30329, USA
5Hope Clinic of Emory Vaccine Center, Emory University, Decatur, GA 30030, USA
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Rafi Ahmed
2Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
3Department of Microbiology and Immunology, Emory University, Atlanta, GA 30322, USA
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Jens Wrammert
1Center for Childhood Infections and Vaccines of Children’s Healthcare of Atlanta, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
2Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
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  • For correspondence: mehul.s.suthar@emory.edu jwramme@emory.edu
Mehul S. Suthar
1Center for Childhood Infections and Vaccines of Children’s Healthcare of Atlanta, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
2Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
3Department of Microbiology and Immunology, Emory University, Atlanta, GA 30322, USA
7Yerkes National Primate Research Center, Atlanta, GA 30329, USA
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  • For correspondence: mehul.s.suthar@emory.edu jwramme@emory.edu
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SUMMARY

The emergence of SARS-CoV-2 variants with mutations in the spike protein is raising concerns about the efficacy of infection- or vaccine-induced antibodies to neutralize these variants. We compared antibody binding and live virus neutralization of sera from naturally infected and spike mRNA vaccinated individuals against a circulating SARS-CoV-2 B.1 variant and the emerging B.1.351 variant. In acutely-infected (5-19 days post-symptom onset), convalescent COVID-19 individuals (through 8 months post-symptom onset) and mRNA-1273 vaccinated individuals (day 14 post-second dose), we observed an average 4.3-fold reduction in antibody titers to the B.1.351-derived receptor binding domain of the spike protein and an average 3.5-fold reduction in neutralizing antibody titers to the SARS-CoV-2 B.1.351 variant as compared to the B.1 variant (spike D614G). However, most acute and convalescent sera from infected and all vaccinated individuals neutralize the SARS-CoV-2 B.1.351 variant, suggesting that protective immunity is retained against COVID-19.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Lead contact: Mehul S. Suthar (mehul.s.suthar{at}emory.edu)

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted February 22, 2021.
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Reduced binding and neutralization of infection- and vaccine-induced antibodies to the B.1.351 (South African) SARS-CoV-2 variant
Venkata Viswanadh Edara, Carson Norwood, Katharine Floyd, Lilin Lai, Meredith E. Davis-Gardner, William H. Hudson, Grace Mantus, Lindsay E. Nyhoff, Max W. Adelman, Rebecca Fineman, Shivan Patel, Rebecca Byram, Dumingu Nipuni Gomes, Garett Michael, Hayatu Abdullahi, Nour Beydoun, Bernadine Panganiban, Nina McNair, Kieffer Hellmeister, Jamila Pitts, Joy Winters, Jennifer Kleinhenz, Jacob Usher, James B. O’Keefe, Anne Piantadosi, Jesse J. Waggoner, Ahmed Babiker, David S. Stephens, Evan J. Anderson, Srilatha Edupuganti, Nadine Rouphael, Rafi Ahmed, Jens Wrammert, Mehul S. Suthar
bioRxiv 2021.02.20.432046; doi: https://doi.org/10.1101/2021.02.20.432046
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Reduced binding and neutralization of infection- and vaccine-induced antibodies to the B.1.351 (South African) SARS-CoV-2 variant
Venkata Viswanadh Edara, Carson Norwood, Katharine Floyd, Lilin Lai, Meredith E. Davis-Gardner, William H. Hudson, Grace Mantus, Lindsay E. Nyhoff, Max W. Adelman, Rebecca Fineman, Shivan Patel, Rebecca Byram, Dumingu Nipuni Gomes, Garett Michael, Hayatu Abdullahi, Nour Beydoun, Bernadine Panganiban, Nina McNair, Kieffer Hellmeister, Jamila Pitts, Joy Winters, Jennifer Kleinhenz, Jacob Usher, James B. O’Keefe, Anne Piantadosi, Jesse J. Waggoner, Ahmed Babiker, David S. Stephens, Evan J. Anderson, Srilatha Edupuganti, Nadine Rouphael, Rafi Ahmed, Jens Wrammert, Mehul S. Suthar
bioRxiv 2021.02.20.432046; doi: https://doi.org/10.1101/2021.02.20.432046

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