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Mechanical mapping of mammalian follicle development using Brillouin microscopy

View ORCID ProfileChii Jou Chan, Carlo Bevilacqua, Robert Prevedel
doi: https://doi.org/10.1101/2021.02.21.432113
Chii Jou Chan
1Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
2Mechanobiology Institute, National University of Singapore, Singapore; Department of Biological Sciences, National University of Singapore, Singapore
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  • For correspondence: dbschii@nus.edu.sg prevedel@embl.de
Carlo Bevilacqua
1Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
3Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences.
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Robert Prevedel
1Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
4Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
5Epigenetics and Neurobiology Unit, European Molecular Biology Laboratory, Monterotondo, Italy
6Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory, Heidelberg, Germany
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  • For correspondence: dbschii@nus.edu.sg prevedel@embl.de
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Abstract

In early mammalian development, the maturation of follicles containing the immature oocytes is an important biological process as the functional oocytes provide the bulk genetic and cytoplasmic materials for successful reproduction. Despite recent work demonstrating the regulatory role of mechanical stress in oocyte growth, quantitative studies of ovarian mechanical properties remain lacking both in vivo and ex vivo. In this work, we quantify the material properties of ooplasm, follicles and connective tissues in intact mouse ovaries at distinct stages of follicle development using Brillouin microscopy, a non-invasive tool to probe mechanics in three-dimensional (3D) tissues. We find that the ovarian cortex and its interior stroma have distinct material properties associated with extracellular matrix deposition, and that intra-follicular mechanical compartments emerge during follicle maturation. Our work provides a novel approach to study the role of mechanics in follicle morphogenesis and pave the way for future understanding of mechanotransduction in reproductive biology, with potential implications for infertility diagnosis and treatment.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Author order corrected on bioRxiv to match manuscript. No content changes to the manuscript.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted February 22, 2021.
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Mechanical mapping of mammalian follicle development using Brillouin microscopy
Chii Jou Chan, Carlo Bevilacqua, Robert Prevedel
bioRxiv 2021.02.21.432113; doi: https://doi.org/10.1101/2021.02.21.432113
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Mechanical mapping of mammalian follicle development using Brillouin microscopy
Chii Jou Chan, Carlo Bevilacqua, Robert Prevedel
bioRxiv 2021.02.21.432113; doi: https://doi.org/10.1101/2021.02.21.432113

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