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Young and Undamaged rMSA Improves the Longevity of Mice

Jiaze Tang, Anji Ju, Boya Li, Shaosen Zhang, Yuanchao Gong, Boyuan Ma, Yi Jiang, Hongyi Liu, Yan Fu, Yongzhang Luo
doi: https://doi.org/10.1101/2021.02.21.432135
Jiaze Tang
1The National Engineering Laboratory for Anti-Tumor Protein Therapeutics, Tsinghua University, Beijing, China
2Beijing Key Laboratory for Protein Therapeutics, Tsinghua University, Beijing, China
3Cancer Biology Laboratory, School of Life Sciences, Tsinghua University, Beijing, China
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Anji Ju
1The National Engineering Laboratory for Anti-Tumor Protein Therapeutics, Tsinghua University, Beijing, China
2Beijing Key Laboratory for Protein Therapeutics, Tsinghua University, Beijing, China
3Cancer Biology Laboratory, School of Life Sciences, Tsinghua University, Beijing, China
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Boya Li
1The National Engineering Laboratory for Anti-Tumor Protein Therapeutics, Tsinghua University, Beijing, China
2Beijing Key Laboratory for Protein Therapeutics, Tsinghua University, Beijing, China
3Cancer Biology Laboratory, School of Life Sciences, Tsinghua University, Beijing, China
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Shaosen Zhang
1The National Engineering Laboratory for Anti-Tumor Protein Therapeutics, Tsinghua University, Beijing, China
2Beijing Key Laboratory for Protein Therapeutics, Tsinghua University, Beijing, China
3Cancer Biology Laboratory, School of Life Sciences, Tsinghua University, Beijing, China
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Yuanchao Gong
1The National Engineering Laboratory for Anti-Tumor Protein Therapeutics, Tsinghua University, Beijing, China
2Beijing Key Laboratory for Protein Therapeutics, Tsinghua University, Beijing, China
3Cancer Biology Laboratory, School of Life Sciences, Tsinghua University, Beijing, China
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Boyuan Ma
1The National Engineering Laboratory for Anti-Tumor Protein Therapeutics, Tsinghua University, Beijing, China
2Beijing Key Laboratory for Protein Therapeutics, Tsinghua University, Beijing, China
3Cancer Biology Laboratory, School of Life Sciences, Tsinghua University, Beijing, China
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Yi Jiang
1The National Engineering Laboratory for Anti-Tumor Protein Therapeutics, Tsinghua University, Beijing, China
2Beijing Key Laboratory for Protein Therapeutics, Tsinghua University, Beijing, China
3Cancer Biology Laboratory, School of Life Sciences, Tsinghua University, Beijing, China
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Hongyi Liu
1The National Engineering Laboratory for Anti-Tumor Protein Therapeutics, Tsinghua University, Beijing, China
2Beijing Key Laboratory for Protein Therapeutics, Tsinghua University, Beijing, China
3Cancer Biology Laboratory, School of Life Sciences, Tsinghua University, Beijing, China
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Yan Fu
1The National Engineering Laboratory for Anti-Tumor Protein Therapeutics, Tsinghua University, Beijing, China
2Beijing Key Laboratory for Protein Therapeutics, Tsinghua University, Beijing, China
3Cancer Biology Laboratory, School of Life Sciences, Tsinghua University, Beijing, China
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  • For correspondence: yluo@tsinghua.edu.cn fuyan@tsinghua.edu.cn
Yongzhang Luo
1The National Engineering Laboratory for Anti-Tumor Protein Therapeutics, Tsinghua University, Beijing, China
2Beijing Key Laboratory for Protein Therapeutics, Tsinghua University, Beijing, China
3Cancer Biology Laboratory, School of Life Sciences, Tsinghua University, Beijing, China
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  • For correspondence: yluo@tsinghua.edu.cn fuyan@tsinghua.edu.cn
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Abstract

Improvement of longevity is an eternal dream of human beings. Here we report that a single protein recombinant mouse serum albumin (rMSA) improved the lifespan and healthspan of C57BL/6N mice. The median lifespan extensions were 17.6% for female and 20.3% for male, respectively. The grip strength of rMSA-treated female and male mice increased by 29.6% and 17.4%, respectively. Meanwhile, the percentage of successful escape increased 23.0% in rMSA-treated male mice using the Barnes Maze test. The rMSA used in this study is young and almost undamaged. We define the concept “young and undamaged” to any protein without any unnecessary modifications by four parameters: intact free thiol (if any), no advanced glycation end-product, no carbonylation, and no homocysteinylation. Here “young and undamaged” rMSA is much younger and less damaged than the endogenous serum albumin from young mice at 1.5 months of age. We predict that young and undamaged proteins altogether can further improve the longevity.

Competing Interest Statement

The authors have declared no competing interest.

  • Abbreviations

    HSA
    human serum albumin.
    AGE
    advanced glycation end-product.
    Hcy
    homocysteine.
    rMSA
    recombinant mouse serum albumin.
    p-tau
    phosphorylated microtubule-associated protein tau.
    MYH1
    myosin heavy chain I.
    α-SMA
    α-smooth muscle actin.
    COL1A1
    collagen I.
    HCP
    host cell proteins.
    qRT-PCR
    quantitative RT-PCR.
    ELISA
    enzyme-linked immunosorbent assay.
    GAPDH
    glyceraldehyde 3-phosphate dehydrogenase.
    DAPI
    4′,6-diamidino-2-phenylindole.
    DTNB
    5, 5’-Dithiobis-(2-nitrobenzoic acid).
    Q-TOF
    Quadrupole-Time of Flight.
    K-S test
    Kolmogorov-Smirnov test.
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    Posted February 21, 2021.
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    Young and Undamaged rMSA Improves the Longevity of Mice
    Jiaze Tang, Anji Ju, Boya Li, Shaosen Zhang, Yuanchao Gong, Boyuan Ma, Yi Jiang, Hongyi Liu, Yan Fu, Yongzhang Luo
    bioRxiv 2021.02.21.432135; doi: https://doi.org/10.1101/2021.02.21.432135
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    Young and Undamaged rMSA Improves the Longevity of Mice
    Jiaze Tang, Anji Ju, Boya Li, Shaosen Zhang, Yuanchao Gong, Boyuan Ma, Yi Jiang, Hongyi Liu, Yan Fu, Yongzhang Luo
    bioRxiv 2021.02.21.432135; doi: https://doi.org/10.1101/2021.02.21.432135

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