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The contribution of electrostatics to hydrogen exchange in the unfolded protein state

Rupashree Dass, Enrico Corlianò, View ORCID ProfileFrans A. A. Mulder
doi: https://doi.org/10.1101/2021.02.22.432104
Rupashree Dass
1Aarhus University
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Enrico Corlianò
2University of Florence
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Frans A. A. Mulder
1Aarhus University
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  • ORCID record for Frans A. A. Mulder
  • For correspondence: fmulder@chem.au.dk
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Abstract

Although electrostatics have long been recognized to play an important role in hydrogen exchange (HX) with solvent, the quantitative assessment of its magnitude in the unfolded state has hitherto been lacking. This limits the utility of HX as a quantitative method to study protein stability, folding and dynamics. Using the intrinsically disordered human protein α-synuclein as a proxy for the unfolded state, we show that a hybrid mean-field approach can effectively compute the electrostatic potential at all backbone amide positions along the chain. From the electrochemical potential a fourfold reduction in hydroxide concentration near the protein backbone is predicted for the C-terminal domain, a prognosis that is in direct agreement with experimentally-derived protection factors from NMR spectroscopy. Thus, impeded HX for the C-terminal region of α-synuclein is not the result of intramolecular hydrogen bonding and/or structure formation.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted February 23, 2021.
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The contribution of electrostatics to hydrogen exchange in the unfolded protein state
Rupashree Dass, Enrico Corlianò, Frans A. A. Mulder
bioRxiv 2021.02.22.432104; doi: https://doi.org/10.1101/2021.02.22.432104
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The contribution of electrostatics to hydrogen exchange in the unfolded protein state
Rupashree Dass, Enrico Corlianò, Frans A. A. Mulder
bioRxiv 2021.02.22.432104; doi: https://doi.org/10.1101/2021.02.22.432104

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