Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The Role of Interspecies recombinations in the evolution of antibiotic-resistant pneumococci

View ORCID ProfileJoshua C. D’Aeth, Mark P.G. van der Linden, View ORCID ProfileLesley McGee, Herminia De Lencastre, View ORCID ProfilePaul Turner, Jae-Hoon Song, Stephanie W. Lo, View ORCID ProfileRebecca A. Gladstone, Raquel Sá-Leão, View ORCID ProfileKwan Soo Ko, View ORCID ProfileWilliam P. Hanage, View ORCID ProfileBernard Beall, View ORCID ProfileStephen D. Bentley, View ORCID ProfileNicholas J. Croucher, The GPS Consortium
doi: https://doi.org/10.1101/2021.02.22.432219
Joshua C. D’Aeth
1MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Joshua C. D’Aeth
Mark P.G. van der Linden
2Institute for Medical Microbiology, National Reference Center for Streptococci, University Hospital, RWTH Aachen, Pauwelsstrasse 30, Aachen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lesley McGee
3Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Lesley McGee
Herminia De Lencastre
4Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paul Turner
5Cambodia-Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap, Cambodia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Paul Turner
Jae-Hoon Song
6Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, South Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephanie W. Lo
7Pathogens & Microbes, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rebecca A. Gladstone
7Pathogens & Microbes, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Rebecca A. Gladstone
Raquel Sá-Leão
4Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kwan Soo Ko
6Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, South Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kwan Soo Ko
William P. Hanage
8Center for Communicable Disease Dynamics, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for William P. Hanage
Bernard Beall
3Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Bernard Beall
Stephen D. Bentley
7Pathogens & Microbes, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Stephen D. Bentley
Nicholas J. Croucher
1MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Nicholas J. Croucher
  • For correspondence: n.croucher@imperial.ac.uk
7Pathogens & Microbes, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Data/Code
  • Preview PDF
Loading

Abstract

The evolutionary histories of the antibiotic-resistant Streptococcus pneumoniae lineages PMEN3 and PMEN9 were reconstructed using global collections of genomes. In PMEN3, one resistant clade spread worldwide, and underwent 25 serotype switches, enabling evasion of vaccine-induced immunity. In PMEN9, only 9 switches were detected, and multiple resistant lineages emerged independently and circulated locally. In Germany, PMEN9’s expansion correlated significantly with the macrolide:penicillin consumption ratio. These isolates were penicillin sensitive but macrolide resistant, through a homologous recombination that integrated Tn1207.1 into a competence gene, preventing further diversification via transformation. Analysis of a species-wide dataset found 183 acquisitions of macrolide resistance, and multiple gains of the tetracycline-resistant transposon Tn916, through homologous recombination, often originating in other streptococcal species. Consequently, antibiotic selection preserves atypical recom- bination events that cause sequence divergence and structural variation throughout the S. pneumoniae chromosome. These events reveal the genetic exchanges between species normally counter-selected until perturbed by clinical interventions.

Competing Interest Statement

NJC has consulted for Antigen Discovery Inc. NJC has received an investigator-initiated award from GlaxoSmithKline.

Footnotes

  • https://doi.org/10.6084/m9.figshare.c.5306462.v1

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license.
Back to top
PreviousNext
Posted February 22, 2021.
Download PDF

Supplementary Material

Data/Code
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The Role of Interspecies recombinations in the evolution of antibiotic-resistant pneumococci
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The Role of Interspecies recombinations in the evolution of antibiotic-resistant pneumococci
Joshua C. D’Aeth, Mark P.G. van der Linden, Lesley McGee, Herminia De Lencastre, Paul Turner, Jae-Hoon Song, Stephanie W. Lo, Rebecca A. Gladstone, Raquel Sá-Leão, Kwan Soo Ko, William P. Hanage, Bernard Beall, Stephen D. Bentley, Nicholas J. Croucher, The GPS Consortium
bioRxiv 2021.02.22.432219; doi: https://doi.org/10.1101/2021.02.22.432219
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
The Role of Interspecies recombinations in the evolution of antibiotic-resistant pneumococci
Joshua C. D’Aeth, Mark P.G. van der Linden, Lesley McGee, Herminia De Lencastre, Paul Turner, Jae-Hoon Song, Stephanie W. Lo, Rebecca A. Gladstone, Raquel Sá-Leão, Kwan Soo Ko, William P. Hanage, Bernard Beall, Stephen D. Bentley, Nicholas J. Croucher, The GPS Consortium
bioRxiv 2021.02.22.432219; doi: https://doi.org/10.1101/2021.02.22.432219

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (2533)
  • Biochemistry (4977)
  • Bioengineering (3486)
  • Bioinformatics (15232)
  • Biophysics (6910)
  • Cancer Biology (5395)
  • Cell Biology (7753)
  • Clinical Trials (138)
  • Developmental Biology (4539)
  • Ecology (7159)
  • Epidemiology (2059)
  • Evolutionary Biology (10234)
  • Genetics (7517)
  • Genomics (9794)
  • Immunology (4863)
  • Microbiology (13234)
  • Molecular Biology (5144)
  • Neuroscience (29465)
  • Paleontology (203)
  • Pathology (838)
  • Pharmacology and Toxicology (1466)
  • Physiology (2142)
  • Plant Biology (4756)
  • Scientific Communication and Education (1013)
  • Synthetic Biology (1338)
  • Systems Biology (4014)
  • Zoology (768)