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Dense Functional and Molecular Readout of a Circuit Hub in Sensory Cortex

Cameron Condylis, Abed Ghanbari, Nikita Manjrekar, Karina Bistrong, Shenqin Yao, Zizhen Yao, Thuc Nghi Nguyen, Hongkui Zeng, View ORCID ProfileBosiljka Tasic, Jerry L. Chen
doi: https://doi.org/10.1101/2021.02.23.432355
Cameron Condylis
1Department of Biomedical Engineering, Boston University, Boston MA 02215, USA
2Center for Neurophotonics, Boston University, Boston MA 02215, USA
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Abed Ghanbari
3Department of Biology, Boston University, Boston MA 02215, USA
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Nikita Manjrekar
3Department of Biology, Boston University, Boston MA 02215, USA
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Karina Bistrong
3Department of Biology, Boston University, Boston MA 02215, USA
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Shenqin Yao
4Allen Institute for Brain Science, Seattle, WA 98109, USA
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Zizhen Yao
4Allen Institute for Brain Science, Seattle, WA 98109, USA
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Thuc Nghi Nguyen
4Allen Institute for Brain Science, Seattle, WA 98109, USA
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Hongkui Zeng
4Allen Institute for Brain Science, Seattle, WA 98109, USA
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Bosiljka Tasic
4Allen Institute for Brain Science, Seattle, WA 98109, USA
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  • ORCID record for Bosiljka Tasic
Jerry L. Chen
1Department of Biomedical Engineering, Boston University, Boston MA 02215, USA
2Center for Neurophotonics, Boston University, Boston MA 02215, USA
3Department of Biology, Boston University, Boston MA 02215, USA
5Center for Systems Neuroscience, Boston University, Boston MA 02215, USA
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  • For correspondence: jerry@chen-lab.org
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ABSTRACT

Information processing in the neocortex is carried out by neuronal circuits composed of different cell types. Recent census of the neocortex using single cell transcriptomic profiling has uncovered more than 100 putative cell types which subdivide major classes of excitatory and inhibitory neurons into distinct subclasses. The extent to which this molecular classification predicts distinct functional roles during behavior is unclear. Here, we combined population recordings using two-photon calcium imaging with spatial transcriptomics using multiplexed fluorescent in situ hybridization to achieve dense functional and molecular readout of cortical circuits during behavior. We characterized task-related responses across major transcriptomic neuronal subclasses and types in layer 2/3 of primary somatosensory cortex as mice performed a tactile working memory task. We find that as neurons are segregated into increasingly discrete molecular types, their task-related properties continue to differentiate. We identify an excitatory cell type, Baz1a, that is highly driven by tactile stimuli. Baz1a neurons homeostatically maintain stimulus responsiveness during altered sensory experience and show persistent enrichment of subsets of immediately early genes including Fos. Measurements of functional and anatomical connectivity reveal that upper layer 2/3 Baz1a neurons preferentially innervate somatostatin-expressing inhibitory neurons. We propose that this connection motif reflects a sensory-driven circuit hub that orchestrates local sensory processing in superficial layers of the neocortex.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted February 23, 2021.
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Dense Functional and Molecular Readout of a Circuit Hub in Sensory Cortex
Cameron Condylis, Abed Ghanbari, Nikita Manjrekar, Karina Bistrong, Shenqin Yao, Zizhen Yao, Thuc Nghi Nguyen, Hongkui Zeng, Bosiljka Tasic, Jerry L. Chen
bioRxiv 2021.02.23.432355; doi: https://doi.org/10.1101/2021.02.23.432355
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Dense Functional and Molecular Readout of a Circuit Hub in Sensory Cortex
Cameron Condylis, Abed Ghanbari, Nikita Manjrekar, Karina Bistrong, Shenqin Yao, Zizhen Yao, Thuc Nghi Nguyen, Hongkui Zeng, Bosiljka Tasic, Jerry L. Chen
bioRxiv 2021.02.23.432355; doi: https://doi.org/10.1101/2021.02.23.432355

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