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Comprehensive evaluation of ACE2 expression in female ovary by single-cell RNA-seq analysis

Siming Kong, Zhiqiang Yan, Peng Yuan, Xixi Liu, Yidong Chen, Ming Yang, Wei Chen, Shi Song, Jie Yan, Liying Yan, Jie Qiao
doi: https://doi.org/10.1101/2021.02.23.432460
Siming Kong
aCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
cKey Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China
dBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China
fPeking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
hAcademy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
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Zhiqiang Yan
aCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
cKey Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China
dBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China
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Peng Yuan
aCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
cKey Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China
dBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China
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Xixi Liu
aCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
cKey Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China
dBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China
eBeijing Advanced Innovation Center for Genomics, Beijing 100871, China
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Yidong Chen
aCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
cKey Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China
dBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China
fPeking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
hAcademy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
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Ming Yang
aCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
cKey Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China
dBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China
fPeking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
hAcademy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
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Wei Chen
aCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
cKey Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China
dBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China
fPeking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
hAcademy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
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Shi Song
aCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
cKey Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China
dBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China
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Jie Yan
aCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
bNational Clinical Research Center for Obstetrics and Gynecology, Beijing 100191, China
cKey Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China
dBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China
eBeijing Advanced Innovation Center for Genomics, Beijing 100871, China
gResearch Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest
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Liying Yan
aCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
bNational Clinical Research Center for Obstetrics and Gynecology, Beijing 100191, China
cKey Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China
dBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China
eBeijing Advanced Innovation Center for Genomics, Beijing 100871, China
gResearch Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest
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  • For correspondence: yanliyingkind@aliyun.com
Jie Qiao
aCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
bNational Clinical Research Center for Obstetrics and Gynecology, Beijing 100191, China
cKey Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China
dBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China
eBeijing Advanced Innovation Center for Genomics, Beijing 100871, China
fPeking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
gResearch Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest
hAcademy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
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  • For correspondence: jie.qiao@263.net
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Abstract

Pneumonia induced by severe acute respiratory coronavirus 2 (SARS-CoV-2) via ACE2 receptor may affect many organ systems like lung, heart and kidney. An autopsy report revealed positive SARS-Cov-2 detection results in ovary, however, the developmental-stage-specific and cell-type-specific risk in fetal primordial germ cells (PGCs) and adult women ovary remained unclear. In this study, we used single-cell RNA-sequencing (scRNA-seq) datasets spanning several developmental stages of ovary including PGCs and cumulus-oocyte complex (COC) to investigate the potential risk of SARS-CoV-2 infection. We found that PGCs and COC exhibited high ACE2 expression. More importantly, the ratio of ACE2-positive cells was sharply up-regulated in primary stage and ACE2 was expressed in all oocytes and cumulus cells in preovulatory stage, suggesting the possible risk of SARS-CoV-2 infection in follicular development. CatB/L, not TMPRSS2, was identified to prime for SARS-CoV-2 entry in follicle. Our findings provided insights into the potential risk of SARS-CoV-2 infection during folliculogenesis in adulthood and the possible risk in fetal PGCs.

Competing Interest Statement

The authors have declared no competing interest.

  • Abbreviations

    SARS-CoV-2
    severe acute respiratory coronavirus 2
    SARS
    Severe acute respiratory syndrome
    PGCs
    primordial germ cells
    ACE2
    angiotensin-converting enzyme2
    CatB/L
    cathepsin B and L
    scRNA-seq
    single-cell RNA-sequencing
    COC
    cumulus-oocyte complex.
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    Posted February 23, 2021.
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    Comprehensive evaluation of ACE2 expression in female ovary by single-cell RNA-seq analysis
    Siming Kong, Zhiqiang Yan, Peng Yuan, Xixi Liu, Yidong Chen, Ming Yang, Wei Chen, Shi Song, Jie Yan, Liying Yan, Jie Qiao
    bioRxiv 2021.02.23.432460; doi: https://doi.org/10.1101/2021.02.23.432460
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    Comprehensive evaluation of ACE2 expression in female ovary by single-cell RNA-seq analysis
    Siming Kong, Zhiqiang Yan, Peng Yuan, Xixi Liu, Yidong Chen, Ming Yang, Wei Chen, Shi Song, Jie Yan, Liying Yan, Jie Qiao
    bioRxiv 2021.02.23.432460; doi: https://doi.org/10.1101/2021.02.23.432460

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