Intrinsic variability of fluorescence calibrators impacts the assignment of MESF or ERF values to nanoparticles and extracellular vesicles by flow cytometry

Estefanía Lozano-Andrés; Tina Van Den Broeck; Lili Wang; Majid Mehrpouyan; Ye Tian; Xiaomei Yan; Marca H.M. Wauben; Ger. J.A. Arkesteijn

doi:10.1101/2021.03.01.433358

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Intrinsic variability of fluorescence calibrators impacts the assignment of MESF or ERF values to nanoparticles and extracellular vesicles by flow cytometry

View ORCID ProfileEstefanía Lozano-Andrés, Tina Van Den Broeck, View ORCID ProfileLili Wang, Majid Mehrpouyan, Ye Tian, View ORCID ProfileXiaomei Yan, View ORCID ProfileMarca H.M. Wauben, View ORCID ProfileGer. J.A. Arkesteijn

doi: https://doi.org/10.1101/2021.03.01.433358

Estefanía Lozano-Andrés

1Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands

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ORCID record for Estefanía Lozano-Andrés

Tina Van Den Broeck

2BD Biosciences, Erembodegem, Belgium

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Lili Wang

3Biosystems and Biomaterials Division, National Institutes of Standards and Technology (NIST), Gaithersburg, MD 20899

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Majid Mehrpouyan

4BD Biosciences, San Jose, CA 95131

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Ye Tian

5Department of Chemical Biology, MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, People’s Republic of China

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Xiaomei Yan

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Marca H.M. Wauben

1Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands

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For correspondence: m.h.m.wauben@uu.nl

Ger. J.A. Arkesteijn

1Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands

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Posted November 30, 2022.

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Intrinsic variability of fluorescence calibrators impacts the assignment of MESF or ERF values to nanoparticles and extracellular vesicles by flow cytometry

Estefanía Lozano-Andrés, Tina Van Den Broeck, Lili Wang, Majid Mehrpouyan, Ye Tian, Xiaomei Yan, Marca H.M. Wauben, Ger. J.A. Arkesteijn

bioRxiv 2021.03.01.433358; doi: https://doi.org/10.1101/2021.03.01.433358

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Intrinsic variability of fluorescence calibrators impacts the assignment of MESF or ERF values to nanoparticles and extracellular vesicles by flow cytometry

Estefanía Lozano-Andrés, Tina Van Den Broeck, Lili Wang, Majid Mehrpouyan, Ye Tian, Xiaomei Yan, Marca H.M. Wauben, Ger. J.A. Arkesteijn

bioRxiv 2021.03.01.433358; doi: https://doi.org/10.1101/2021.03.01.433358

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