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A Comparison of Performance for Different SARS-Cov-2 Sequencing Protocols

View ORCID ProfileJuanjo Bermúdez
doi: https://doi.org/10.1101/2021.03.01.433428
Juanjo Bermúdez
1Contignant Technologies SL c/Emigrant 30, Barcelona, Spain (08906)
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  • ORCID record for Juanjo Bermúdez
  • For correspondence: juanjo75es@gmail.com
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Abstract

SARS-Cov-2 genome sequencing has been identified as a fundamental tool for fighting the COVID-19 pandemic. It is used, for example, for identifying new variants of the virus and for elaborating phylogenetic trees that help to trace the spread of the virus. In the present study we provide a comprehensive comparison between the quality of the assemblies obtained from different sequencing protocols. We demonstrate how some protocols actively promoted by different high-level administrations are inefficient and how less-used alternative protocols show a significant increased performance. This increase of performance could lead to cheaper sequencing protocols and therefore to a more convenient escalation of the sequencing efforts around the world.

Competing Interest Statement

I am the developer and the owner of all the rights of the s-aligner software.

Footnotes

  • https://contignant.com

  • https://doi.org/10.5281/zenodo.4558343

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted March 01, 2021.
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A Comparison of Performance for Different SARS-Cov-2 Sequencing Protocols
Juanjo Bermúdez
bioRxiv 2021.03.01.433428; doi: https://doi.org/10.1101/2021.03.01.433428
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A Comparison of Performance for Different SARS-Cov-2 Sequencing Protocols
Juanjo Bermúdez
bioRxiv 2021.03.01.433428; doi: https://doi.org/10.1101/2021.03.01.433428

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