Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Cytoplasmic domain and enzymatic activity of ACE2 is not required for PI4KB dependent endocytosis entry of SARS-CoV-2 into host cells

Hang Yang, Xiaohui Zhao, Meng Xun, Lingjie Xu, Bing Liu, Hongliang Wang
doi: https://doi.org/10.1101/2021.03.01.433503
Hang Yang
1Department of pathogen biology and immunology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an, 710061, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiaohui Zhao
1Department of pathogen biology and immunology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an, 710061, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Meng Xun
1Department of pathogen biology and immunology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an, 710061, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lingjie Xu
2Emergency Department of West China Hospital, Sichuan University, Chengdu, 610041, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bing Liu
3BioBank, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710061, China
4Instrument Analysis Center of Xi’an Jiaotong University, Xi’an,710048, China
5Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, SW7 2AZ London, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: hongliangwang@xjtu.edu.cn bliu@imperial.ac.uk
Hongliang Wang
1Department of pathogen biology and immunology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an, 710061, China
6Key Laboratory of Environment and Genes Related to Diseases, Xi’an Jiaotong University, Xi’an, 710061, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: hongliangwang@xjtu.edu.cn bliu@imperial.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

The recent COVID-19 pandemic poses a global health emergency. Cellular entry of the causative agent SARS-CoV-2 is mediated by its spike protein interacting with cellular receptor- human angiotensin converting enzyme 2 (ACE2). Here, we used lentivirus based pseudotypes bearing spike protein to demonstrate that entry of SARS-CoV-2 into host cells is dependent on clathrin-mediated endocytosis, and phosphoinositides play essential role during this process. In addition, we showed that the intracellular domain and the catalytic activity of ACE2 is not required for efficient virus entry. These results provide new insights into SARS-CoV-2 cellular entry and present potential targets for drug development.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted March 02, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Cytoplasmic domain and enzymatic activity of ACE2 is not required for PI4KB dependent endocytosis entry of SARS-CoV-2 into host cells
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Cytoplasmic domain and enzymatic activity of ACE2 is not required for PI4KB dependent endocytosis entry of SARS-CoV-2 into host cells
Hang Yang, Xiaohui Zhao, Meng Xun, Lingjie Xu, Bing Liu, Hongliang Wang
bioRxiv 2021.03.01.433503; doi: https://doi.org/10.1101/2021.03.01.433503
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Cytoplasmic domain and enzymatic activity of ACE2 is not required for PI4KB dependent endocytosis entry of SARS-CoV-2 into host cells
Hang Yang, Xiaohui Zhao, Meng Xun, Lingjie Xu, Bing Liu, Hongliang Wang
bioRxiv 2021.03.01.433503; doi: https://doi.org/10.1101/2021.03.01.433503

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (2646)
  • Biochemistry (5259)
  • Bioengineering (3671)
  • Bioinformatics (15788)
  • Biophysics (7247)
  • Cancer Biology (5624)
  • Cell Biology (8088)
  • Clinical Trials (138)
  • Developmental Biology (4763)
  • Ecology (7510)
  • Epidemiology (2059)
  • Evolutionary Biology (10571)
  • Genetics (7727)
  • Genomics (10125)
  • Immunology (5187)
  • Microbiology (13896)
  • Molecular Biology (5383)
  • Neuroscience (30754)
  • Paleontology (215)
  • Pathology (876)
  • Pharmacology and Toxicology (1524)
  • Physiology (2253)
  • Plant Biology (5017)
  • Scientific Communication and Education (1040)
  • Synthetic Biology (1384)
  • Systems Biology (4145)
  • Zoology (811)