Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Structural basis of LhcbM5-mediated state transitions in green algae

Xiaowei Pan, Ryutaro Tokutsu, Anjie Li, Kenji Takizawa, Chihong Song, Kazuyoshi Murata, Tomohito Yamasaki, Zhenfeng Liu, Jun Minagawa, Mei Li
doi: https://doi.org/10.1101/2021.03.02.433643
Xiaowei Pan
1National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, P.R. China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ryutaro Tokutsu
2National Institute for Basic Biology, National Institutes of Natural Sciences, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, 444-8585, Japan
3Department of Basic Biology, School of Life Science, the Graduate University for Advanced Studies, SOKENDAI, Okazaki 444-8585, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anjie Li
1National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, P.R. China
4University of Chinese Academy of Sciences, Beijing 100049, P.R. China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kenji Takizawa
2National Institute for Basic Biology, National Institutes of Natural Sciences, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, 444-8585, Japan
7Astrobiology Center, National Institutes of Natural Sciences, 2-21-1 Osawa, Mitaka, Tokyo, 181-8588, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chihong Song
5Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, 38 Nishigonaka, Myodaiji, Okazaki, 444-8585, Japan
6National Institute for Physiological Sciences, National Institutes of Natural Sciences, 38 Nishigonaka, Myodaiji, Okazaki, 444-8585, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kazuyoshi Murata
5Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, 38 Nishigonaka, Myodaiji, Okazaki, 444-8585, Japan
6National Institute for Physiological Sciences, National Institutes of Natural Sciences, 38 Nishigonaka, Myodaiji, Okazaki, 444-8585, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tomohito Yamasaki
8Science and Technology Department, Natural Science Cluster, Kochi University, 2-5-1 Akebono-cho, Kochi 780-8520, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhenfeng Liu
1National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, P.R. China
4University of Chinese Academy of Sciences, Beijing 100049, P.R. China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: liuzf@ibp.ac.cn minagawa@nibb.ac.jp meili@ibp.ac.cn
Jun Minagawa
2National Institute for Basic Biology, National Institutes of Natural Sciences, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, 444-8585, Japan
3Department of Basic Biology, School of Life Science, the Graduate University for Advanced Studies, SOKENDAI, Okazaki 444-8585, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: liuzf@ibp.ac.cn minagawa@nibb.ac.jp meili@ibp.ac.cn
Mei Li
1National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, P.R. China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: liuzf@ibp.ac.cn minagawa@nibb.ac.jp meili@ibp.ac.cn
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

In green algae and plants, state transitions serve as a short-term light acclimation process to regulate light harvesting capacity of photosystems I and II (PSI and PSII). During the process, a portion of the light-harvesting complexes II (LHCII) are phosphorylated, dissociate from PSII and bind PSI to form PSI-LHCI-LHCII supercomplex. Here we report high-resolution structures of PSI-LHCI-LHCII supercomplex from Chlamydomonas reinhardtii, revealing the mechanism of assembly between PSI-LHCI complex and two phosphorylated LHCII trimers containing all four types of LhcbM proteins. Two specific LhcbM isoforms, namely LhcbM1 and LhcbM5, directly interact with the PSI core through their phosphorylated amino-terminal regions. Furthermore, biochemical and functional studies on mutant strains lacking either LhcbM1 or LhcbM5 indicate that only LhcbM5 is indispensable in the supercomplex formation. The results unraveled the specific interactions and potential excitation energy transfer routes between green algal PSI and two phosphorylated LHCIIs.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted March 02, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Structural basis of LhcbM5-mediated state transitions in green algae
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Structural basis of LhcbM5-mediated state transitions in green algae
Xiaowei Pan, Ryutaro Tokutsu, Anjie Li, Kenji Takizawa, Chihong Song, Kazuyoshi Murata, Tomohito Yamasaki, Zhenfeng Liu, Jun Minagawa, Mei Li
bioRxiv 2021.03.02.433643; doi: https://doi.org/10.1101/2021.03.02.433643
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Structural basis of LhcbM5-mediated state transitions in green algae
Xiaowei Pan, Ryutaro Tokutsu, Anjie Li, Kenji Takizawa, Chihong Song, Kazuyoshi Murata, Tomohito Yamasaki, Zhenfeng Liu, Jun Minagawa, Mei Li
bioRxiv 2021.03.02.433643; doi: https://doi.org/10.1101/2021.03.02.433643

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (2646)
  • Biochemistry (5259)
  • Bioengineering (3671)
  • Bioinformatics (15788)
  • Biophysics (7247)
  • Cancer Biology (5624)
  • Cell Biology (8088)
  • Clinical Trials (138)
  • Developmental Biology (4763)
  • Ecology (7510)
  • Epidemiology (2059)
  • Evolutionary Biology (10571)
  • Genetics (7727)
  • Genomics (10125)
  • Immunology (5187)
  • Microbiology (13896)
  • Molecular Biology (5383)
  • Neuroscience (30754)
  • Paleontology (215)
  • Pathology (876)
  • Pharmacology and Toxicology (1524)
  • Physiology (2253)
  • Plant Biology (5017)
  • Scientific Communication and Education (1040)
  • Synthetic Biology (1384)
  • Systems Biology (4145)
  • Zoology (811)