Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

CaSR modulates sodium channel-mediated Ca2+-dependent excitability

Briana J. Martiszus, Timur Tsintsadze, Wenhan Chang, View ORCID ProfileStephen M. Smith
doi: https://doi.org/10.1101/2021.03.03.433701
Briana J. Martiszus
1Section of Pulmonary & Critical Care Medicine, VA Portland Health Care System, Portland, Oregon, USA
2Department of Medicine, Division of Pulmonary & Critical Care Medicine, Oregon Health & Science University, Portland, Oregon, 97239, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Timur Tsintsadze
1Section of Pulmonary & Critical Care Medicine, VA Portland Health Care System, Portland, Oregon, USA
2Department of Medicine, Division of Pulmonary & Critical Care Medicine, Oregon Health & Science University, Portland, Oregon, 97239, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wenhan Chang
3Endocrine Research Unit, Veterans Affairs Medical Center and University of California, San Francisco, California, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephen M. Smith
1Section of Pulmonary & Critical Care Medicine, VA Portland Health Care System, Portland, Oregon, USA
2Department of Medicine, Division of Pulmonary & Critical Care Medicine, Oregon Health & Science University, Portland, Oregon, 97239, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Stephen M. Smith
  • For correspondence: smisteph@ohsu.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Increasing extracellular [Ca2+] ([Ca2+]o) strongly decreases intrinsic excitability in neurons but the mechanism is unclear. By one hypothesis, [Ca2+]o screens surface charge reducing voltage-dependent sodium channel (VGSC) activation and by another [Ca2+]o activates Calcium-sensing receptor (CaSR) closing the sodium-leak channel (NALCN). Here we report that action potential (AP) firing rates increased in wild-type (WT), but not CaSR null mutant (Casr-/-) neocortical neurons, following the switch from physiological to reduced Ca2+-containing Tyrode. However, after membrane potential correction, AP firing increased similarly in both genotypes inconsistent with CaSR regulation of NALCN. Activation of VGSCs was the dominant contributor to the increase in excitability after the [Ca2+]o change. VGSC conductance-voltage relationships were hyperpolarized by decreasing [Ca2+]o for Casr-/- neurons indicating CaSR contributes to [Ca2+]o-dependent excitability via VGSCs. Regulation of VGSC gating by [Ca2+]o is the key mechanism mediating [Ca2+]o-dependent changes in neocortical neuron excitability and CaSR influences neuronal excitability by its effects on VGSC gating.

Introduction

Excitable tissues are strongly regulated by extracellular [Ca2+] ([Ca2+]o) (Neher and Sakaba, 2008; Ma et al., 2014; Jackman and Regehr, 2017). Movement of extracellular Ca2+, through voltage-activated Ca2+ channels (VACC), to the intracellular space is central to many of these processes (Nanou and Catterall, 2018). However, a distinct, extracellular mechanism that is independent of synaptic transmission also contributes to [Ca2+]o-dependent regulation of nerve and muscle function (Weidmann, 1955; Frankenhaeuser, 1957; Frankenhaeuser and Hodgkin, 1957). Decreases in [Ca2+]o substantially facilitates action potential generation increasing intrinsic excitability(Weidmann, 1955; Frankenhaeuser, 1957; Frankenhaeuser and Hodgkin, 1957). In the brain, physiological neuronal activity decreases [Ca2+]o (Nicholson et al., 1978) leading to further increases in action potential firing in neighboring neurons (Anderson et al., 2013). The firing patterns and computational properties of local circuits are impacted substantially by this positive feedback leading to changes in brain behaviors (Titley et al., 2019). Furthermore under pathological conditions, larger decreases in [Ca2+]o occur, resulting in even greater changes in circuit activity, and implicating [Ca2+]o-dependent excitability in the pathogenesis of brain injury (Ayata and Lauritzen, 2015).

Classical studies proposed that the mechanism underlying [Ca2+]o-dependent excitability centers on voltage-gated sodium channel (VGSC) sensitivity to extracellular Ca2+. External Ca2+ was proposed to interact with local negative charges on the extracellular face of the membrane or ion channels thereby increasing the potential field experienced by VGSCs and reducing the likelihood of VGSC activation at the resting membrane potential (Frankenhaeuser and Hodgkin, 1957; Hille, 1968). Reductions in [Ca2+]o decrease the screening of the surface potential, reverse the membrane stabilization and facilitate VGSC activation (Frankenhaeuser and Hodgkin, 1957; Hille, 1968). This surface potential screening model accounted for [Ca2+]o-dependent excitability in nerves and muscle without a need for additional molecular players and was widely accepted (Hille, 2001). However, the theory was challenged by new data demonstrating that activation of the sodium leak channel (NALCN), a non-selective cation channel, by the intracellular proteins, UNC79 and UNC80 (Lu et al., 2009; Lu et al., 2010) was necessary for [Ca2+]o-dependent excitability to occur in hippocampal neurons. Following the deletion of NALCN or UNC79, [Ca2+]o-dependent excitability was completely lost suggesting the increased excitability resulted from the activation of the non-rectifying NALCN which depolarized neurons and increased the likelihood of action potential generation independent of changes in VGSC function (Lu et al., 2010). The calcium-sensing receptor (CaSR), a G-protein coupled receptor (GPCR), was hypothesized to detect and transduce the [Ca2+]o changes and signal to the downstream multistep pathway(Lu et al., 2010). CaSR is well-positioned as a candidate [Ca2+]o detector because at nerve terminals it detects [Ca2+]o and regulates a non-selective cation channel(Smith et al., 2004; Chen et al., 2010) and because it transduces changes in [Ca2+]o into NALCN activity following heterologous co-expression of CaSR, NALCN, UNC79, and UNC80 (Lu et al., 2010). Interest in the UNC79-UNC80-NALCN pathway has also risen, due to its essential role in the maintenance of respiration(Lu et al., 2007), the regulation of circadian rhythms (Lear et al., 2013; Flourakis et al., 2015), and because mutations of UNC80 and NALCN cause neurodevelopmental disorders, characterized by development delay and hypotonia (Al-Sayed et al., 2013; Perez et al., 2016).

Here we address the question of whether the G-protein mediated NALCN pathway or VGSCs transduce the [Ca2+]o-dependent effects on excitability. We test if CaSR is a modulator of neuronal excitability via its action on a nonselective cation channel, determine the impact of CaSR expression on factors of intrinsic neuronal excitability, and examine the relative contributions of [Ca2+]o-regulated changes on VGSC and NALCN gating. In recordings from neocortical neurons, isolated by pharmacological block of excitatory and inhibitory inputs, we determine that neuronal firing is increased by decreasing external divalent concentrations and that this is almost entirely attributable to [Ca2+]o-dependent shifts in VGSC gating. Surprisingly, CaSR deletion substantially shifted VGSC gating, but had no effect on NALCN sensitivity to [Ca2+]o. Taken together our experiments indicate that acute [Ca2+]o-dependent increases in neuronal excitability result from changes in VGSC and NALCN gating and that CaSR contributes by an, as yet, uncharacterized action on VGSCs.

Results

CaSR and [Ca2+]o-dependent neuronal excitability

The elimination of [Ca2+]o-dependent excitability in neurons by deletion of UNC79 or NALCN challenged the long-standing hypothesis that local or diffuse surface charge screening of VGSCs mediated these effects(Lu et al., 2010). But how were changes in external divalent ion concentrations transduced to UNC79 and NALCN? We tested if CaSR provided the link, by comparing neuronal excitability in wild-type (WT) and nestin cre-recombinase expressing CaSR null-mutant (creCasr-/-) neurons that were genotyped by PCR (see Methods)(Chang et al., 2008). Quantification by RT-qPCR indicated >98% reduction in the Casr expression levels in neocortical cultures produced from creCasr-/- mice compared to cre-positive WT (creWT; Figure 1-supplement). Current clamp recordings were performed to measure the intrinsic spontaneous action potential firing rate from pharmacologically-isolated, cultured, neocortical neurons (glutamatergic and GABAergic activity blocked by 10 µM CNQX, 50 µM APV, and 10 µM Gabazine respectively). After establishing the whole-cell configuration, we measured the action potential firing rates of conventional WT (conWT), creWT, and creCasr-/- neurons in physiological Tyrode solution (; containing 1.1 mM [Ca2+] and 1.1 mM [Mg2+]) at the resting membrane potential (RMP) and then in reduced Ca2+ and Mg2+ Tyrode (T0.2; containing 0.2 mM [Ca2+] and [Mg2+]). The reduction in [Ca2+]o and [Mg2+]o caused a small depolarization and an increase in spontaneous action potential firing in both WT (con- and cre-) neurons within 15 seconds of the solution change that was substantially attenuated in the creCasr-/- neuron (Figure 1A, middle row). Changing back to physiological external divalent concentrations reversed this effect within 10 s (Figure 1A, lower row). The pooled data from similar experiments indicated that on average the conWT and creWT neurons were equally sensitive to decreased [Ca2+]o and [Mg2+]o and had similar basal levels of activity (Figure 1B-D). Two-way repeated measures (RM) ANOVA confirmed a significant interaction indicating the response to changes in [Ca2+]o and [Mg2+]o were dependent on genotype (F (2,54) = 3.193, P = 0.049, Supplementary Table 1). Post-hoc tests (Sidak compensated for multiple comparisons here and in all later tests) confirmed that the reduction in [Ca2+]o and [Mg2+]o substantially increased action potential frequency in conWT and creWT but not creCasr-/- neurons (Figure 1B, P =0.0009, <0.0001, and =0.6697, respectively). These data indicate that CaSR loss substantially reduces [Ca2+]o-dependent excitability in neurons.

Supplementary Figure 1
  • Download figure
  • Open in new tab
Supplementary Figure 1

Casr expression levels shown as delta delta with actin used as normalizing gene. Average values of 1.0, 0.02, and 0.65 for creWT, creCasr-/-, and conWT respectively with each genotype reflecting the average data from six cultures each represented by triplicate samples. The Kruskal-Wallis test indicates differences between genotypes (P = 0.0002) with Dunn’s multiple comparison showing Casr expression levels are lower in creCasr-/- (P = 0.0016), but not conWT (P = 0.7739), than in creWT neocortical cultures.

Figure 1:
  • Download figure
  • Open in new tab
Figure 1: CaSR deletion reduces [Ca2+]o-dependent excitability

A) Spontaneous voltage traces at RMP following the application of solutions with different divalent concentrations (1.1 (upper traces), T0.2 (middle), and recovery (lower)) recorded in three individual neurons with or without CaSR (conWT (black), creWT (blue) and creCasr-/- (red)). CreCasr-/-Each trace depicts 150 s of continuous acquisition.

B) Histograms of average action potential (AP) frequency (Hz) recorded using the same solutions:, T0.2, and recovery. Individual recordings represented by open circles linked with lines and average is represented with a bar. From left to right graphs depict conWT (n = 18), creWT (n = 21), and CreCasr-/- (n = 18). ANOVA:

Post-hoc tests (Sidak compensated for multiple comparisons here and in all later figures) showed that AP frequency increased in conWT (P = 0.0009) and cre WT (P, 0.0001), but not CaSr-/- (P = 0.6697) neurons when changing from to T0.2.

C) Baseline average action potential frequency in. was unaffected by genotype (P > 0.999)

D) Average action potential frequency with T0.2 application was the same in conWT and creWT (P = 0.9831) and higher than in creCasr-/- neurons (P = 0.013 and 0.0033 respectively). E) Histogram summarizing effects of [Ca2+]o on AP frequency in creWT and Casr-/- neurons after a current injection to counter [Ca2+]o-dependent depolarization following T0.2 application. Two-way RM ANOVA indicates that reducing [Ca2+]o increases the AP frequency (F (1, 35) = 11.54, P = 0.0017) and that this is significant in the creWT but not creCasr-/-neurons (P = 0.0075 and 0.1555 for 21 and 16 recordings respectively). Inset shows average membrane potential after the current injection.

F) Histogram summarizing effects of [Ca2+]o on AP frequency in creWT and Casr-/- neurons after current injection in to depolarize membrane potential to value recorded in T0.2. Two-way RM ANOVA indicates that reducing [Ca2+]o increases the AP frequency (F (1, 35) = 45.09, P = 0.0004) and that this is significant in the creWT but not creCasr-/- neurons (P = 0.0044 and 0.056 for 21 and 16 recordings respectively). Inset shows average membrane potential after the current injection.

G) Exemplar action potentials elicited by current injection in a creWT (blue) and a creCasr-/- neuon (red) in (unbroken) and T0.2 (broken). Action potential threshold is indicated by + for the first AP elicited by current injection (50 to 200 pA) under the same conditions as panel E.

H) Plot of average AP threshold in and T0.2 in creWT and creCasr-/- neurons elicited as per panel G. Two-way RM ANOVA indicates that reducing [Ca2+]o hyperpolarized the AP threshold (F (1, 27) = 56.48, P < 0.0001) but that genotype had no effect (F (1, 27) = 2.284, P = 0.1424). [Ca2+]o was highly effective in both creWT and creCasr-/-neurons (P < 0.0001 and P = 0.0003 for 19 and 10 recordings respectively). Individual neuron values are represented by open circles linked by lines and averages by filled circles.

I) Plot of effect of [Ca2+]o and CaSR on RMP. Two-way RM ANOVA indicates that increasing [Ca2+]o (F (1, 37) = 31.65, P < 0.0001) and CaSR deletion (F (1, 37) = 19.1, P < 0.0001) hyperpolarized the RMP without an interaction (F (1, 37) = 1.035, P = 0.3155). Post-hoc testing indicated RMP was depolarized with the switch to T0.2 in both creWT and creCasr-/-neurons (P < 0.0001 and P = 0.0066 for 21 and 18 recordings respectively).

CaSR-independent mechanisms contribute to [Ca2+]o-dependent signaling

If CaSR-regulated NALCN-dependent depolarization is entirely responsible for the extracellular divalent-sensitive changes in neuronal excitability (Lu et al., 2010) then CaSR signaling to NALCN would depolarize the membrane potential when extracellular calcium was reduced. The same hypothesis predicts that reversal of this depolarization should prevent (or block) the increase in excitability seen in T0.2. To test this prediction we measured action potential frequency following current injections to drive the membrane potential in low [Ca2+]o conditions to match the RMP seen during physiological [Ca2+]o conditions (unique to each neuron). Action potential frequency in T0.2 was reduced by the hyperpolarization but neurons remained sensitive to changes in [Ca2+]o (Figure 1E, Suppl. Table 2; F (1,35)=11.54, P=0.0017, 2-way RM ANOVA) indicating mechanisms besides NALCN were involved. Post-hoc tests confirmed this retained sensitivity to [Ca2+]o and [Mg2+]o was restricted to creWT and not creCasr-/-- neurons, (Figure 1E; P=0.0075 and =0.156, respectively). Similarly, in the reciprocal experiment in which the membrane potential in was depolarized to match that at low divalent concentration, the decrease in external divalent concentration increased action potential frequency (Figure 1F, Suppl. Table 3; F (1,35)=15.17, P=0.0004, 2-way RM ANOVA), and this was significant in creWT but not creCasr-/- neurons (Figure 1F, P = 0.004). Ineffective matching of the membrane potential following solution changes did not account for the persistence of [Ca2+]o-dependent excitability (insets, Figure 1E,F). These data indicate a mechanism in addition to CaSR-NALCN-mediated depolarization must contribute to the extracellular divalent-sensitive changes in neuronal excitability.

Does CaSR modulate RMP and [Ca2+]o-dependent depolarization?

We tested if there was an endogenous difference in the excitability of creWT and creCasr-/- neurons to explain the retained sensitivity to divalents by measuring the action potential threshold. Action potentials were elicited in and T0.2 using minimal current injection (50-250 pA) and the threshold measured as the point at which dV/dt reached 20 mV/ms (Figure 1G, membrane potential-corrected as in Figure 1 E). The action potential threshold was hyperpolarized from −48.6 ± 0.7 mV to −54.3 ± 1.1 mV with the switch from to T0.2 in creWT neurons (Figure 1H) which would have increased excitability. However, the same effect was observed in creCasr-/- neurons (−50.9 ± 0.86 mV to −55.4 ± 2.1 mV; F (1,27)=56.48, P < 0.0001, 2-way RM ANOVA, Suppl. Table 4). The lack of effect of CaSR on AP threshold (F (1, 27) = 2.284, P = 0.142) in these experiments, indicated the reduced divalent sensitivity of creCasr-/- (Figure 1E,F) was not simply due to altered action potential threshold.

We next asked if differences in RMP in and the response of RMP to T0.2 could contribute to the different divalent-dependent excitability of creWT and creCasr-/- neurons. Genotype and external divalent concentrations were both significant determinants of RMP (zero current injection; 2-way RM ANOVA, (Suppl. Table 5, F (1,37)=19.1, P < 0.0001 and F (1,37)=31.65, P < 0.0001, respectively). As CaSR deletion did not affect AP threshold (Figure 1H), action potential generation presumably occurred more frequently in the creWT neurons due to the relatively depolarized membrane potential (8 mV positive than creCasr-/- neurons, Figure 1I,).

If CaSR-mediated NALCN-dependent depolarization is sufficient to account for [Ca2+]o-dependent excitability then creWT but not creCasr-/- neurons, should depolarize in response to decreased [Ca2+]o. However, the RMP of creWT and creCasr-/- neurons both depolarized similarly (Figure 1I; 5.6 ± 1.1 mV, P < 0.0001 and 3.9 ± 1.2 mV, P = 0.0066 respectively) when T0.2 was applied indicating the existence of a [Ca2+]o-sensitive pathway in creCasr-/- neurons. Overall these data support the idea that CaSR played a substantial role in mediating divalent dependent changes in excitability, but that WT neurons also possessed CaSR-independent mechanisms to fully account for the [Ca2+]o-dependent excitability.

CaSR-independent mechanisms contribute to [Ca2+]o-dependent signaling

Further mechanistic complexity was suggested by the effects of CaSR and [Ca2+]o on RMP. This lead to a number of additional questions including: does the difference in RMP contribute to the difference in [Ca2+]o-dependent excitability between creWT and creCasr-/- neurons, how do decreases in [Ca2+]o depolarize creCasr-/- neurons, and is this pathway present in creWT neurons? To address the first of these questions, we compared the response of creWT and creCasr-/- neurons to changes in extracellular divalent concentrations after removing the confounding variation in RMP. After establishing a stable current-clamp recording in we injected a standing current (Ia) until the resting membrane potential was −70 mV. We then recorded for 50 s before switching the bath solution to T0.2. As before, there was a small depolarization followed by an increase in action potential frequency in creWT neurons (Figure 2A,B). To test if this increase in excitability was fully attributable to [Ca2+]o-dependent depolarization we adjusted the standing current (Ib) until the membrane potential was −70 mV and then measured the action potential frequency (Figure 2C). In the exemplar, AP firing was reduced by the hyperpolarization but remained higher in T0.2 at −70 mV than in at −70 mV (Figure 2A-C) confirming CaSR-mediated depolarization was not acting alone to increase the excitability (c.f. Figure 1I). The creCasr-/- neurons responded similarly to T0.2 and hyperpolarization (Figure 2A-C) indicating the effect was not mediated by CaSR. We compared the average effects of at −70 mV with Ia, T0.2 with Ia, and T0.2 at −70 mV with Ib on creWT and creCasr-/- genotypes (Figure 2D, Suppl. Table 6) using a 2-way RM ANOVA. Extracellular divalent concentration and membrane potential substantially affected action potential frequency (F (3, 87) = 17.97, P < 0.0001). CaSR deletion did not impact the response to extracellular divalent concentration when creWT and creCasr-/- neuron recordings were started at a membrane potential of −70 mV l (F (1, 29) = 0.2005, P = 0.6577). Post-hoc testing showed that excitability was increased in T0.2 compared with regardless which of the two holding currents were used (Figure 2D; Suppl. Table 7). The depolarization of Casr-/- neurons in response to T0.2 was unexpected (Figure 1I) since initially CaSR-NALCN signaling was hypothesized as the mechanism for [Ca2+]o-dependent excitability(Lu et al., 2010). After injection of Ia to set the membrane potential to −70 mV, the switch from to T0.2 significantly depolarized the membrane potential (Figure 2E; Suppl. Table 8, Two-way RM ANOVA, F (1, 29) = 29.22, P < 0.0001) as did CaSR deletion (F (1, 29) = 4.874, P = 0.0353). Post-hoc testing indicate that the membrane potential in T0.2 was more depolarized in the creCasr-/- than in creWT neurons (Figure 2B,E; - 65.6 ±1.6 mV vs −59.4 ± 2.4 mV, P = 0.0083). Taken together, these experiments indicate CaSR-NALCN signaling was not contributing to the difference in [Ca2+]o-dependent excitability between creWT and creCasr-/- neurons but that these differences may be due to genotype-dependent differences in RMP or intrinsic excitability.

Figure 2:
  • Download figure
  • Open in new tab
Figure 2: CaSR deletion does not affect [Ca2+]o-dependent excitability at equivalent membrane potential

A) Exemplary traces showing the [Ca2+]o-dependent increase in neuronal excitability following the switch from to T0.2 (change indicated by upper trace) in creWT (blue) and cre casr-/- (red) neurons when initial membrane potentials matched at −70 mV.

B) Expanded view of the final five seconds of traces in A illustrating sustained depolarization from −70 mV following T0.2 application.

C) Exemplary traces showing the [Ca2+]o-dependent decrease in neuronal excitability following the switch from T0.2 to (change indicated by upper trace) in creWT (blue) and cre casr-/- (red) neurons when initial membrane potentials matched at −70 mV. Same recording as A.

D) Histogram of average [Ca2+]o-dependent changes in action potential frequency (Hz) in creWT (blue) and cre casr-/- (red) neurons when initial voltage is −70 mV in (Ia) or T0.2 (Ib). Two-way RM ANOVA performed after logarithmic transformation indicates that reducing [Ca2+]o increases the AP frequency (F (3, 87) = 17.97, P < 0.0001) similarly in creWT and creCasr-/- neurons (F (1, 29) = 0.2005, P = 0.6577). Post-hoc tests indicate significant differences between AP frequency in and T0.2 regardless of the holding current but not between AP frequency recorded at different holding currents and the same solutions (Ia or Ib; Supplementary Table?).

E) Membrane potential depolarization following the switch to T0.2 from. Two-way RM ANOVA indicates that reducing [Ca2+]o (F (1, 29) = 29.22, P < 0.0001) and CaSR deletion (F (1, 29) = 4.874, P = 0.0353) significantly depolarized the membrane potential but that there was no interaction (F (1, 29) = 4.055, P = 0.0534). Post-hoc testing indicate that membrane potentials were matched using current injection in (−70.5 ± 0.4 mV and −70.2 ± 0.2 mV for creWT and creCasr-/- neurons respectively, P = 0.985) but different in T0.2 (−65.6 ± 1.6 mV and –59.4 ± 2.4 mV, P = 0.0083).

F) Exemplar action potentials elicited by current injection from −70 mV in a creWT (blue) and a creCasr-/- neuron (red) in solutions (unbroken) and T0.2 (broken). Action potential threshold is indicated by + for the first AP elicited by current injection (50 to 200 pA).

G) Plot of average AP threshold in and T0.2 in creWT and creCasr-/- neurons, elicited as per panel F here and in subsequent panels. Two-way RM ANOVA indicates that reducing [Ca2+]o hyperpolarized the AP threshold (F (1, 25) = 51.66, P < 0.0001) whereas CaSR deletion had the opposite effect (F (1, 25) = 10.52, P = 0.0033). There was no interaction (Supplementary Table). Post-hoc tests indicate that the AP thresholds in solutions and T0.2 were depolarized similarly by CaSR deletion (5.3 ± 2.0 mV and 5.5 ± 2.0 mV, P = 0.020 and 0.017) in creWT and creCasr-/- neurons respectively.

H) Plot of average AP half-duration in and T0.2 in creWT and creCasr-/- neurons. Two-way RM ANOVA indicates that reducing [Ca2+]o prolonged the AP half-duration (F (1, 28) = 19.73, P = 0.0001).

I) Plot of average AP peak in and T0.2 in creWT and creCasr-/- neurons. The AP peaks were higher in T1.1 and in Casr-/- neurons (Supplementary Table 9C).

Voltage-gated sodium channels contribute to [Ca2+]o-dependent excitability

Reversal of the [Ca2+]o-dependent depolarization did not completely block the increased excitability associated with the switch to T0.2 (Figures 1E,F & 2D) indicating another mechanism other than [Ca2+]o-dependent NALCN was responsible. We tested if voltage-gated channels were contributing by to [Ca2+]o-dependent excitability by examining action potential threshold in neurons held at a membrane potential of −70 mV. AP threshold was hyperpolarized by 8 mV on average following the change from to T0.2 in creWT and creCasr-/- neurons (Figure 2F,G, Suppl. Table 9A; F (1, 25) = 51.66, P< 0.0001). Furthermore, the AP threshold was relatively depolarized in the creCasr-/- neurons in and T0.2 (5.3 ± 2.0 mV (P = 0.020) and 5.5 ± 2.0 mV (P = 0.017) respectively), indicating creWT neurons possessed increased excitability and increased sensitivity to decreases in external divalent concentration (Figure 2F,G). The action potential half-width recorded under the same conditions, was also sensitive to deletion of CaSR and reduction of [Ca2+]o (Figure 2H,I Suppl. Table 9B). ANOVA indicated that the switch to T0.2 from broadened AP half-width (F (1,28) = 19.7, P = 0.0001) but this effect was restricted to creWT (P = 0.0004) and not creCasr-/- (P = 0.117) neurons. The genotype and [Ca2+]o interacted to both affect AP peak voltage (Figure 2I, Suppl. Table 9C; F (1, 28) = 6.76, P = 0.015) with the peak potential being reduced by T0.2 in the creWT (P < 0.0001) but not creCasr-/- neurons (P = 0.34).

We examined the properties of VGSCs and voltage-gated potassium channels (VGPCs) to determine the reason for the altered AP threshold. VGSCs were isolated in neocortical neurons and the current-voltage characteristics examined. Families of VGSC currents were activated in neurons after 2-4 weeks in culture. Maximum VGSC currents were elicited at −30 mV and averaged −8.0 ± 0.8 nA (n=7) and −8.8 ± 2.8 nA (n=6) in creWT and creCasr-/- neurons respectively. The current-voltage curve shifted in a hyperpolarizing direction with the switch from to T0.2 but extensive neuronal processes limited the quality of the voltage-clamp and prevented useful analysis. We next examined VGSC gating in nucleated outside-out patches(Sather et al., 1992; Almog et al., 2018) to ensure better voltage control. VGSC currents were elicited by voltage steps from −80 mV (10 mV increments to 40 mV). In T0.2, the VGSC inactivation (see below) resulted in smaller currents that were more sensitive to depolarization (bold traces elicited by steps to −50 mV, Figure 3A) as previously observed (Frankenhaeuser and Hodgkin, 1957; Campbell and Hille, 1976; Armstrong and Cota, 1991). [Ca2+]o sensitivity was confirmed in the normalized current-voltage plot for both creWT (blue, n= 8) and creCasr-/- (red, n= 11) neurons (Figure 3A,C). VGSC current inactivation was studied using a test pulse to −20 mV, each of which was preceded by a conditioning step (100 ms) to between −140 mV and −20 mV. In we observed less inactivation than in T0.2 (Figure 3B, bold traces show currents elicited following prepulse to −80 mV). We compared the effects of [Ca2+]o and CaSR deletion on VGSC current inactivation using plots of normalized conductance and measuring the half maximal voltage (V0.5) (circles, Figure 3D,E). The reduction in [Ca2+]o (F (1, 18) = 56, P < 0.0001) left-shifted V0.5 (2-way RM ANOVA, Suppl. Table 10) but deletion did not CaSR (F (1, 18) = 0.563, P = 0.463). The switch from to T0.2 shifted V0.5 by −19 and −21 mV in creWT and creCasr-/- respectively (P < 0.0001 for both). We also tested how the VGSC activation properties were affected by CaSR and [Ca2+]o. The peak inward VGSC currents (Figure 3B,D) were divided by the driving voltage and then plotted as conductance-voltage plots. The normalized conductance plots (squares, Figure 3E,F) indicate that the switch from to T0.2 significantly facilitated VGSC activation (V0.5 was hyperpolarized by 10 mV) (F (1, 17) = 98, P < 0.0001; 2-way RM ANOVA, Suppl. Table 11) consistent with VGSCs in other excitable cells (Hille, 2001). Switching from to T0.2 shifted V0.5 by −10 mV and −9 mV in creWT and creCasr-/- neurons respectively (P < 0.0001 in both). Unexpectedly, the V0.5 for VGSC activation was also impacted by CaSR deletion, the relative depolarization reducing the likelihood of VGSC activation (F (1, 17) = 4.8, P = 0.04) in the Casr-/- neurons (Figure 3I) Overlap of the inactivation and activation conductance plots represents the voltage range over which persistent VGSC currents, or window currents, are likely to occur(Chadda et al., 2017). [Ca2+]o reduction and CaSR hyperpolarized this region of overlap towards the RMP (Figure 3 F,G insets) increasing the likelihood that persistent VGSC currents were activated at resting membrane potential and therefore contributing to [Ca2+]o-dependent excitability.

Figure 3
  • Download figure
  • Open in new tab
Figure 3 CaSR deletion and [Ca2+]o affect VGSC current gating

A) Exemplary traces showing VGSC currents activated by voltage steps from −80 in 10 mV increments (left), in nucleated patches isolated from creWT (blue) and creCasr-/- (red) neurons in solutions and T0.2. The VGSC currents elicited by 10 ms depolarizations to - 50 mV (bold) were greater following the switch to T0.2.

B) Exemplary traces showing VGSC currents activated by voltage steps to −20 mV following a 100 ms conditioning step (left), in the same patches as A) using solutions and T0.2. The VGSC currents elicited following conditioning steps to −80 mV (bold) were smaller following the switch to T0.2.

C) Current-voltage plots of average normalized VGSC currents in nucleated patches from creWT (n = 8) and creCasr-/- (n = 11) neurons in (filled circles) and T0.2 (open circles). Currents were normalized using the maximum VGSC current in each recording.

D) Plot of average normalized conductance versus voltage in patches from creWT neurons for activation (square, n = 8) and inactivation (circle, n = 8) in solutions (filled) and T0.2 (open). Boltzmannn curves are drawn using average values from individual fits and gray broken lines indicate V0.5 values for each condition.

E) Plot of average normalized conductance versus voltage in patches from creCasr-/- neurons for activation (square, n = 11) and inactivation (circle, n = 12) in solutions (filled) and T0.2 (open). Boltzmannn curves are drawn using average values from individual fits and gray broken lines indicate V0.5 values for each condition. Inset shows plot expanded to emphasize voltage dependence of the window currents.

F and G represent the plots of D and E expanded to emphasize the voltage dependence of the window currents.

H) Histogram showing V0.5 for VGSC inactivation in and T0.2 in patches from creWT and creCasr-/- neurons.

I) Histogram showing V0.5 for VGSC activation in and T0.2 in patches from creWT and creCasr-/- neurons.

VGPC currents were isolated and recorded in creWT and creCasr-/- neurons in and T0.2 solutions after blocking contaminating currents. Currents were elicited by a series of 60 ms steps from −70 mV to 60 mV in 10 mV increments (Figure 4). The VGPC current amplitudes were measured at the peak and at the end of the depolarizing step (normalized to the value at 60 mV in). Neither the peak nor end current were affected by reduction of the external divalent concentration or by deletion of CaSR (Figure 4) over the range of voltages. The currents activated at 60 mV were similarly unaffected (Figure 4, Two-way RM ANOVA ((3, 57) = 1.347, P= 0.2683 and (1, 19) = 1.231, P= 0.2811, Suppl. Table 12). These data indicate that VGPCs are not involved in [Ca2+]o-dependent excitability in neocortical neurons.

Figure 4
  • Download figure
  • Open in new tab
Figure 4 CaSR deletion and [Ca2+]o do not significantly affect VGPC current gating.

A) Exemplary traces showing VGPC currents activated by voltage steps from −80 in 10 mV increments (left), in a creWT neuron in solutions and T0.2. The outward currents elicited by the 50 ms voltage step were measured at peak and at the end of the step (average of last 5 ms indicated by gray bar).

B) Current voltage-plot of average normalized VGPC currents (n = 10) in creWT neurons in (filled circles) and T0.2 (open circles) at peak or end of step. Currents were normalized using the maximum outward current in each condition here and below.

C) Peak and end outward currents at 60 mV elicited in same neurons as B. Two-way RM ANOVA indicates that peak and outward currents were not different in or T0.2 ((3, 57) = 1.347, P= 0.2683 nor were they affected by CaSR deletion (data from E, (1, 19) = 1.231, P= 0.2811).

D) Current voltage-plot of average normalized VGPC currents (n = 11) in creCasr-/- neurons in (filled circles) and T0.2 (open circles) at peak or end of step.

E) Peak and end outward currents at 60 mV elicited in same neurons as D.

VGSCs are the dominant contributor to [Ca2+]o-dependent currents

To compare the contributions of VGSCs and NALCN to the [Ca2+]o-dependent depolarization seen in neocortical neurons (Figure 2) we measured the size of the currents elicited at −70 mV in neurons following the switch from to T0.2. We used conWT neurons to avoid potential confounding Cre-dependent effects (Qiu et al., 2011). Since NALCN is resistant to the VGSC blocker tetrodotoxin (TTX) (Lu et al., 2007; Swayne et al., 2009) but Gd3+ (10 µM) inhibits NALCN and VGSCs (Elinder and Arhem, 1994; Li and Baumgarten, 2001; Lu et al., 2009), we were able to pharmacologically separate the contributions of VGSCs and NALCN to the basal current following the switch from to T0.2 (−31 ± 3 pA, n=13; Figure 5A-C). Addition of a saturating concentration of TTX (1 µM) in T0.2 inhibited a persistent inward current within a few seconds in all but one of the recordings (Figure 5A-C), consistent with VGSCs contributing to the inward current elicited by T0.2. Switching to plus TTX produced minimal change in the basal current on average (Figure 5C). However, in some neurons elicited an outward current (Figure 5A,C) whereas in others there was an inward current (Figure 5B,C) indicating the presence of two types of TTX resistant [Ca2+]o-dependent pathways. Presumably NALCN was contributing to the [Ca2+]o-dependent TTX-resistant effect observed in Figure 5A. Co-application of Gd3+ (10 µM) following block of VGSCs with TTX, resulted in a small inward deflection of the average basal current in solution and largely inhibited sensitivity to concomitant decreases in [Ca2+]o (Figure 5A-C). The reduced sensitivity of neurons to the reduction of [Ca2+]o in the presence of TTX, suggests that VGSCs are a major contributor to the depolarizing current elicited by low [Ca2+]o.

Figure 5
  • Download figure
  • Open in new tab
Figure 5 VGSC current activation by decreased [Ca2+]o.

A,B) Plots illustrating the responses of the basal currents in two WT neurons during application of and T0.2 before and during TTX or TTX and Gd3+. Average basal currents were measured over 50 ms every 2 s with and T0.2 application indicated by vertical shading (gray represents T0.2) and blockers application by horizontal bars and broken vertical lines.

C) Plot of average basal current measurements (filled circles) and individual neurons (open circles) in each solution condition in conWT (n=13) neurons. Each basal current represents the average value recorded during last 20 s of the specific solution application.

D) Average [Ca2+]o dependent basal currents sensitive to TTX and Gd3+ calculated by subtraction of data in C and the remaining [Ca2+]o dependent current after application of both blockers for conWT neurons.

E) Exemplar traces of currents elicited by 50 ms voltage steps between −100 and −50 mV during application of solutions described in C.

F) Plots of the average currents over the last 5 ms of each voltage step in all six solutions for conWT (n = 13).

G) Plots of the average [Ca2+]o dependent currents derived by subtraction of WT data (F) resolved as total or control (broken blue), in the presence of TTX (broken red), and in the presence of TTX and Gd3+ (remainder green). The TTX-sensitive (solid red), Gd3+-sensitive (solid blue) and NALCN (dotted line) component currents were obtained by further subtraction. Inset shows expanded view at intercept of TTX-sensitive and NALCN components.

H) Exemplars of the TTX- and Gd3+-sensitive [Ca2+]o dependent currents. Broken red line represents zero current line.

Using serial subtraction of the basal currents (Figure 5C), we compared the size of the TTX-sensitive (−28.2 ± 5.3 pA), Gd3+-sensitive (−5.7 ± 3.4 pA) and remaining (3.4 ± 2.0 pA) [Ca2+]o-dependent currents (Figure 5D; RM-ANOVA, F (1.495, 17.94) = 13.30, P = 0.0007, Suppl. Table 13). Multiple comparison testing indicated that the TTX-sensitive [Ca2+]o-dependent current was greater than the Gd3+-sensitive (P = 0.039) and remaining [Ca2+]o-dependent currents (P = 0.0009; Suppl. Table 14). Similar differences in the relative sizes of the TTX-, Gd3+-, and remainder [Ca2+]o-dependent basal current currents were also observed in Casr-/-neurons (Figure 5D). While there were rare neurons in which there was a larger Gd3+-sensitive current (Figure 5C) the reduced sensitivity of neurons to the reduction of [Ca2+]o in the presence of TTX, confirms that VGSCs are the major contributor to the depolarizing current elicited by low [Ca2+]o..

In a fraction of the neurons, an inward deflection of the basal current occurred when [Ca2+]o was increased in the presence of TTX (Figure 5B,C) which contrasted with the outward current expected from NALCN deactivation (Figure 5A). We examined the voltage-dependence of the contributions of VGSCs, NALCN, and this second [Ca2+]o-dependent TTX-resistant current to better characterize [Ca2+]o-dependent excitability. We used 50 ms voltage steps between −100 and −50 mV and averaged the current over the last 5 ms of the step. Three additional major effects are illustrated by the exemplar current traces (Figure 5E). First, in the absence of blockers, the switch from to T0.2 substantially increased the number of large, rapidly inactivating inward currents even at −70 mV following hyperpolarizing steps. Second, in TTX, low [Ca2+]o increased the linear inward and rectifying outward currents. Third, in the presence of TTX and Gd3+ changing between and T0.2 had little effect suggesting Gd3+ is blocking both NALCN and the second [Ca2+]o-dependent TTX-resistant current. These observations were confirmed in the average current-voltage plots (Figure 5F) where it is clear that at −80 to −100 mV the major [Ca2+]o-dependent currents are inward and resistant to TTX and sensitive to Gd3+ whereas at −70 to −50 mV the largest [Ca2+]o-dependent currents are TTX-sensitive. The [Ca2+]o-dependent effects were calculated by subtracting the currents recorded in from those in T0.2 under control conditions (Figure 5G, broken red), in the presence of TTX (broken blue) and TTX plus Gd3+ (solid green). The TTX-sensitive (solid red) and Gd3+-sensitive (solid blue) [Ca2+]o-dependent currents were obtained by additional subtraction (broken red minus broken blue and broken blue minus green). The average [Ca2+]o-dependent current carried by VGSCs only became evident once the neurons were depolarized above −80 mV (Figure 5G). The time course of deactivation of the persistent [Ca2+]o-dependent VGSC currents was observed following hyperpolarization from −70 mV (Figure 5H, middle). At more negative potentials, the Gd3+-sensitive current accounted for all of the [Ca2+]o-dependent current and traces showed an ohmic voltage dependence (Figure 5G). However, the Gd3+-sensitive current reversed at −60 mV and outward currents were elicited by steps to −60 and −50 mV that exhibited a voltage-dependent activation and inactivation (Figure 5H, right-hand). This is consistent with the Gd3+-sensitive current consisting of the sum of NALCN and an outward voltage-dependent current. Assuming conservatively that all of the Gd3+-sensitive current at −100 mV could be attributed to NALCN and employing the channel’s linear voltage-dependence and zero mV reversal potential(Lu et al., 2007; Lu et al., 2010), then the amplitude of NALCN currents could be estimated over the voltage range −100 to 0 mV (broken black line, Figure 5G). By interpolation (Figure 5G, inset), the contribution of NALCN and VGSCs to [Ca2+]o-dependent currents were equal at ∼-77 mV with the contribution from VGSCs increasing with depolarization. A similar analysis of [Ca2+]o-dependent currents in creCasr-/- neurons indicated that the contribution of VGSCs was greater than that of NALCN once membrane potentials were depolarized beyond - 72 mV (Figure 5 Supplement). These data indicate that [Ca2+]o-dependent currents around the resting membrane that contribute to [Ca2+]o-dependent excitability are mainly attributable to VGSCs.

Figure 3 Supplementary figure
  • Download figure
  • Open in new tab
Figure 3 Supplementary figure

Culture age does not impact V0.5 for VGSC current activation (A) and inactivation (B) in patches from creWT and creCasr-/- neurons. The delta (triangles) represents the shift in V0.5 fllowing the switch from T1.1 to T0.2.

Figure 5
  • Download figure
  • Open in new tab
Figure 5 VGSC current activation by decreased [Ca2+]o.

Supp Figure

A) Plot of average basal current measurements (filled circles) and individual neurons (open circles) in each solution condition in creCasr-/- (n = 9) neurons. Each basal current represents the average value recorded during last 20 s of the specific solution application.

B) Plots of average currents versus voltage for creCasr-/- (n = 9) neurons as per Figure 5 F

C) Plots of the average [Ca2+]o dependent currents derived by subtraction of creCasr-/- data as per Figure 5 G.

VGSCs are the dominant contributor to [Ca2+]o-dependent excitability

The complex architecture of neocortical neurons restricted our ability to clamp the membrane potential following the activation of large, rapid VGSC currents. Thus we re-examined the contribution of VGSCs and NALCN to the depolarizations that mediate [Ca2+]o-dependent excitability in current clamp recordings from conWT neurons. Consistent with earlier experiments (Figure 2), switching from to T0.2 depolarized the membrane potential from −70 mV by 7.2 ± 1.5 mV (n = 12) and increased spontaneous action potential firing in pharmacologically isolated neurons (Figure 6A,B). We used TTX and Gd3+ to measure the contributions of VGSCs and NALCN respectively to these [Ca2+]o-dependent depolarizations. TTX blocked action potential generation, as expected, but also hyperpolarized the membrane potential indicating that VGSCs were open in T0.2 (Figure 6A1) and (Figure 6A2). The switch from T0.2 to in TTX resulted in a hyperpolarization, consistent with [Ca2+]o-dependent NALCN closure, in some neurons (Figure 6A1 lower trace and B). Other neurons depolarized with the switch to (Figure 6A2 lower trace and B) consistent with a [Ca2+]o-dependent outward current similar to that observed in Figure 5B,C. On average the [Ca2+]o-dependent depolarization was almost entirely prevented by TTX or TTX and Gd3+ co-application (Figure 6B). The [Ca2+]o-dependent depolarizations in conWT neurons were different for blocker type (1-way RM ANOVA, F (1.219, 13.41) = 12.83, P = 0.0022, Suppl. Table 15) and more sensitive to TTX than Gd3+ or following co-application (P = 0.022 and 0.0028 respectively, Supp. Table 16). On average VGSCs accounted for 93% of the depolarization that triggers [Ca2+]o-dependent excitability in WT neurons starting at −70 mV (Figure 6C) and we observed a similar pattern in Casr-/- neurons (Figure 6C).

Figure 6
  • Download figure
  • Open in new tab
Figure 6 [Ca2+]o-dependent depolarization is almost entirely mediated via VGSCs

A) The response of the membrane potential in two WT neurons during application of and T0.2 before and during TTX or TTX and Gd3+. and T0.2 application is indicated by vertical shading (gray represents T0.2) and blocker applications by horizontal bars and broken vertical lines. The broken red line indicates −70 mV. Voltage-expanded view of the trace illustrates that in the presence of TTX, hyperpolarization (A1) and depolarization (A2) may occur following the switch to. Membrane potential values highlighted by broken red lines.

B) Plot of average (filled circles) and individual (open circles) Ca2+-dependent voltage changes (filled circles) following the switch from to T0.2 (by subtraction of average between-spike membrane potential over the last 10 sec of each solution application). Each solution applied to conWT (n=12) and creCasr-/- (n = 9) neurons.

C) Average [Ca2+]o dependent voltage changes sensitive to TTX and Gd3+ calculated by subtraction of data in B, and the remaining [Ca2+]o dependent voltage after application of both blockers.

D) Estimates of the average relative size of the [Ca2+]o dependent NALCN and VGSC currents in neocortical neurons between −100 and −30 mV. NALCN values from Fig 5G. The [Ca2+]o-dependent VGSC currents were estimated as follows: the products of the VGSC activation and inactivation conductance plots were calculated for T0.2 using the average Boltzmann curves in Figure 3. These were converted to currents (I= driving voltage x conductance), and scaled to match the average [Ca2+]o dependent TTX-sensitive current at −70 mV. The current generated in T0.2 minus that generated in ΔICa) was plotted against membrane voltage.

E) Plot of the fraction of the average [Ca2+]o dependent depolarizing current carried by VGSC derived from D. The change in average resting membrane potential recorded in Figure 1 is indicated by the gray bar.

Next we estimated the average relative contributions of the [Ca2+]o-dependent NALCN and VGSC currents over a wider physiological voltage range. [Ca2+]o-dependent NALCN currents were extrapolated from −100 mV, where contaminating currents appear minimal (Figure 5G) and compared with the [Ca2+]o-dependent VGSC currents predicted from scaled conductance plots (Figure 3D). The VGSC currents were the major contributor to [Ca2+]o-dependent currents over the −77 to −30 mV voltage range (Figure 6 D,E) suggesting VGSCs are likely to dominate those of NALCN at resting membrane potentials observed in T1.1 and T0.2 (Figure 6E, gray bar).

Discussion

Extracellular calcium concentration regulates both synaptic transmission and intrinsic neuronal excitability, thereby strongly affecting the probability of action potential generation. Consequently, physiological and pathological changes in [Ca2+]o will impact neuronal computation in a complex manner. We have investigated the mechanisms underlying [Ca2+]o-dependent changes in intrinsic neuronal excitability and tested if CaSR is transducing decreases in [Ca2+]o into NALCN-mediated depolarizations to trigger action potentials (Lu et al., 2010; Philippart and Khaliq, 2018). We found no evidence that this specific mechanism was active in neocortical neurons (Figure 2). Instead, we determined that the vast majority of [Ca2+]o dependent neuronal excitability was mediated via VGSCs in three ways. First, decreased [Ca2+]o activated VGSCs at the resting membrane potential and depolarized the membrane towards the action potential threshold (Figure 6). Second, decreased [Ca2+]o hyperpolarized the expected VGSC window current towards the membrane potential increasing the likelihood of action potential generation (Figure 3). Third, deletion of CaSR modulated VGSC gating by synergistically increasing the sensitivity of the currents to depolarization via an unidentified mechanism (Figure 3). CaSR also indirectly affected action potential generation by modestly depolarizing the membrane potential (Figure 1). While the actions of [Ca2+]o on VGSCs were responsible for the vast majority of the [Ca2+]o dependent neuronal excitability, using Gd3+ we isolated small [Ca2+]o-dependent inward currents in about half of the neurons (Figure 5). These small Gd2+-sensitive currents presumably reflected activation of NALCN, and were unaffected by CaSR deletion, but their relatively small size compared to TTX-sensitive [Ca2+]o-dependent inward currents indicate that they would be minor contributors to [Ca2+]o-dependent excitability compared to VGSCs (Figures 5,6).

The fractions of the [Ca2+]o-dependent currents and depolarizations that were sensitive to TTX were surprisingly large compared to those that were Gd3+-sensitive (Figures 5D,6C) indicating the relative importance of VGSC- and NALCN-mediated contributions to [Ca2+]o-dependent excitability respectively. The resistance of NALCN to TTX (Lu et al., 2007; Swayne et al., 2009) reassures that the relatively large TTX-sensitive component is due to selective block of VGSC. Persistent subthreshold VGSC currents have been shown to determine spiking rates in other central neurons (Taddese and Bean, 2002; Gorelova and Seamans, 2015) and so the increased VGSC currents we observed in T0.2 are well-positioned to explain the increased action potential frequency (Figure 6). We are unable to determine from these experiments which neuronal compartment is most affected by the change in [Ca2+]o(Gorelova and Seamans, 2015). However, the physiological impact of VGSC-mediated [Ca2+]o-dependent excitability will be enormous overall because of the dynamic nature of [Ca2+]o in vivo where it decreases from basal (1.1-1.2 mM) by 30-80%(Nicholson et al., 1978; Ohta et al., 1997; Pietrobon and Moskowitz, 2014). The overall computational effects of physiological decrements in [Ca2+]o will be complex because the increased action potential generation due to changes on VGSCs (Figure 3,6) will be confounded by the impact of reduced Ca2+ entry through VACCs (Hess et al., 1986; Weber et al., 2010), reduced synaptic transmission (Neher and Sakaba, 2008), and altered CaSR-mediated signaling at the nerve terminal (Phillips et al., 2008; Vyleta and Smith, 2011).

A number of questions arise from our studies. Why was NALCN essential to effect [Ca2+]o-dependent excitability in hippocampal(Lu et al., 2010) but not neocortical neurons (Figure 2)? Since action potentials were elicited by divalent concentration changes alone in neocortical neurons (Figure 2) whereas the response to current injections was the measure of excitability in hippocampal neurons(Lu et al., 2010) could the differences reflect different experimental protocols. We cannot exclude this possibility however, it is unclear how NALCN facilitated acute [Ca2+]o-dependent excitability during depolarizing injections from matched membrane potentials since the current injections were expected to produce similar or smaller depolarizations given NALCN is relatively voltage insensitive (Lu et al., 2009). Could the increased action potential number seen in WT compared to NALCN deficient neurons(Lu et al., 2010) reflect other indirect actions of a persistent leak current (Sokolov et al., 2007) or compensatory changes in ion channel activity(Jun et al., 1999)? It is also surprising that deletion of NALCN or UNC-79 completely ablated [Ca2+]o-dependent excitability in hippocampal neurons (Lu et al., 2010) since, like other excitable cells, hippocampal VGSC currents are sensitive to changes in [Ca2+]o (Isaev et al., 2012). Consequently, if the UNC79-UNC80-NALCN pathway modulates VGSC function this could explain how loss of NALCN or UNC-79 could delete acute [Ca2+]o-dependent changes in VGSC function and excitability. NALCN appears to transduce [Ca2+]o- and G-protein dependent excitability in other neurons(Philippart and Khaliq, 2018) but GPCRs other than CaSR may be involved(Kubo et al., 1998; Tabata and Kano, 2004). Further characterization of the UNC79-UNC80-NALCN signaling pathway is essential given the major changes in neurological function that have been described following mutations of NALCN or upstream co-molecules such as UNC79 and UNC80(Stray-Pedersen et al., 2016; Bourque et al., 2018; Kuptanon et al., 2019).

In a small fraction of the neocortical neurons (Figures 5,6) there was a modest inward current or depolarization with the application of low calcium once VGSCs had been blocked. In a few cases they were sensitive to 10 µM Gd3+ consistent with a NALCN-mediated effect and those that were resistant were consistent with other [Ca2+]o-dependent non-selective cation channels(Ma et al., 2012). Deletion of CaSR, increased [Ca2+]o-dependent depolarizations (Figure 2E, 6B) indicating [Ca2+]o-dependent non-selective cation channels are perhaps upregulated in the absence of CaSR. While CaSR-NALCN signaling did not contribute to [Ca2+]o-dependent excitability in neocortical neurons (Figures 2 and 6) it was clear that Casr-/- neurons were substantially less sensitive to changes [Ca2+]o (Figure 1A,B). The reduced [Ca2+]o sensitivity in these neurons is attributable to the combination of altered VGSC gating (Figure 3E,F) and the hyperpolarized RMP (Figure 1I). Although CaSR did not affect the amplitude of the shift in V0.5 following the switch to T0.2, V0.5 in was hyperpolarized by loss of CaSR (Figure 3EF). Could CaSR stimulation activate G-proteins and regulate the basal V0.5 for VGSC currents (Figure 3E,F)? In neocortical neurons, G-protein activation hyperpolarized VGSC gating and this was blocked by GDPβS (Mattheisen et al., 2018) which is inconsistent with the effect we observed here. Other possible explanations are that CaSR regulates VGSC subunit expression or post translational modification (Cantrell et al., 1996; Zhang et al., 2019). Loss of CaSR could hyperpolarize the neocortical neurons (Figure 1I) by increasing the function of depolarizing components or inhibiting hyperpolarizing elements, but it is unclear which of the many ion channels or pumps mediate this effect on RMP(Tavalin et al., 1997; Talley et al., 2001; Bean, 2007; Harnett et al., 2015; Hu and Bean, 2018).

Overall our studies indicate that [Ca2+]o-dependent excitability in neurons is largely attributable to actions of extracellular calcium on the VGSC function. Given the dynamic nature of brain extracellular calcium, this mechanism is likely to impact neuronal signaling greatly under physiological and pathological conditions. CaSR-dependent reduction of VGSC sensitivity to membrane potential adds further complexity to extracellular calcium signaling and identifies another potential mechanism by which CaSR stimulation increases neuronal death following stroke and traumatic brain injury(Kim et al., 2013; Hannan et al., 2018).

Materials and Methods

Genotyping and CaSR mutant mice

ConWT animals were obtained from a 20 year old colony consisting of a stable strain of C57BL/6J and 129×1 mice. The creCasr-/- mice were generated by breeding floxed Casr (Chang et al., 2008) and nestin Cre mice (B6.Cg-Tg (Nes-cre)1Kln/J, The Jackson Laboratory) as described previously (Sun et al., 2018). The lox sites were positioned to delete Casr exon 7 which resulted in the loss of Casr expression(Chang et al., 2008) and the nestin promoter was designed to ensure floxing occurred in neuronal and glial precursors. The creWT mice were generated by crossing mice that did not contain the flox Casr mutation but did express the nestin Cre mutation. The creCasr-/- and creWT mice were all generated using a background C57BL/6J and 129S4 strain. Tail DNA extraction was performed using the Hot Shot Technique with a 1-2 hour boil(Montero-Pau et al., 2008). The presence or absence of the flox Casr mutation and Cre mutation were confirmed by PCR for each mouse. MoPrimers used for cre PCR were: Nes-Cre 1: GCAAAACAGGCTCTAGCGTTCG, Nes-Cre 2: CTGTTTCACTATCCAGGTTACGG; run on a 1% agarose gel. Primers for lox PCR were: P3U: TGTGACGGAAAACATACTGC, Lox R: GCGTTTTTAGAGGGAAGCAG; run on a 1.5% agarose gel (Chang et al., 2008). Successful deletion of Casr in the neocortical cultures was confirmed by measuring mRNA expression levels with the QuantStudio12K Flex Real-time PCR System (Applied Biosystems)and the TaqMan mouse probe set to Casr (Mm00443377_m1) with ActB (Mm04394036_g1) as the endogenous control (Supplementary Figure 1).

Neuronal Cell Culture

Neocortical neurons were isolated from postnatal day 1–2 mouse pups of either sex as described previously(Phillips et al., 2008). All animal procedures were approved by V.A. Portland Health Care System Institutional Animal Care and Use Committee in accordance with the U.S. Public Health Service Policy on Humane Care and Use of Laboratory Animals and the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Animals were decapitated following induction of general anesthesia with isoflurane and then the cerebral cortices were removed. Cortices were incubated in trypsin and DNase and then dissociated with a heat polished pipette. Dissociated cells were cultured in MEM plus 5% FBS on glass coverslips. Cytosine arabinoside (4 µM) was added 48 hours after plating to limit glial division. Cells were used, unless otherwise stated after ≥14days in culture.

Electrophysiological Recordings

Cells were visualized with a Zeiss IM 35 inverted microscope. Whole-cell voltage-and current-clamp recordings were made from cultured neocortical neurons using a HEKA EPC10 USB amplifier. Except where stated in the text, extracellular Tyrodes solution contained (mM) 150 NaCl, 4 KCl, 10 HEPES, 10 glucose, 1.1 MgCl2, 1.1 CaCl2, pH 7.35 with NaOH. Calcium and magnesium were modified as described in the Figure legends Synaptic transmission was blocked by the addition of (in µM) 10 CNQX, 10 Gabazine, and 50 APV to the bath solution. Most recordings were made using a potassium gluconate intracellular solution containing (mM) 135 K-gluconate, 10 HEPES, 4 MgCl2, 4 NaATP, 0.3 NaGTP, 10 phosphocreatine disodium, pH 7.2 with KOH hydroxide. In nucleated patch experiments the pipette solution contained (in mM) 113 Cesium methane sulfonate, 1.8 EGTA, 10 HEPES, 4 MgCl2, 0.2 CaCl2, 4 NaATP, 0.3 NaGTP, 14 phosphocreatine disodium, pH 7.2 with TEA hydroxide. Electrodes used for recording had resistances ranging from 2 to 7 MΩ. Voltages have been corrected for liquid junction potentials. All experiments were performed at room temperature (21-23 °C).

Data Acquisition and Analysis

Whole-cell voltage-and current-clamp recordings were made using a HEKA EPC10 USB amplifier, filtered at 2.9 kHz using a Bessel filter, and sampled at 20 kHz during injection protocols and 10 kHz during continuous acquisition. Analysis was performed using Igor Pro (Wavemetrics, Lake Oswego, OR) and Minianalysis (Synaptosoft). Data values are reported as mean ± SEM. Statistical tests were performed using GraphPad Prism (6) and P-values < 0.05, 0.01, 0.001, and 0.0001 were indicate with *, **, ***, and ****. All post-hoc tests were Sidak compensated for multiple comparisons. Data were log-transformed to improve normalization in Figure 2D. To ensure non-zero values, minimize bias, and allow logarithmic transformation, each AP frequency measurement was increased by 0.02 as the duration of the recording at −70 mV was 50 s.

Solution Application

Solutions were applied by gravity from a glass capillary (1.2 mm outer diameter) placed ∼1 mm from the neuron under study. Solutions were switched manually using a low dead volume manifold upstream of the glass capillary. CNQX and Gabazine were supplied by Abcam. KB-R7943 Mesylate was supplied by Tocris. Creatine Phosphate was supplied by Santa Cruz Biotech. Cinacalcet was supplied by Toronto Research Chemicals and Tetrodotoxin by Alomone Other reagents were obtained from Sigma-Aldrich.

Supplementary Data

ANOVA and post-hoc analysis statistical tables

View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 1 Action potential frequency
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 2 Action potential frequency
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 3 Action potential frequency
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 4 Action potential threshold
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 5 RMP
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 6 Action potential frequency
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 7 Action potential frequency
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 8 Membrane potential with Ia
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 9A Action potential threshold recorded at −70 mV
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 9B Action potential half-width recorded at −70 mV
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 9C Action potential peak voltage recorded at −70 mV
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 10 Voltage-gated sodium channel current V0.5 for inactivation
View this table:
  • View inline
  • View popup
Supplementary Table 11 Voltage-gated sodium channel current V0.5 for activation
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 12 Voltage-gated potassium channel currents at 60 mV
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 13 [Ca2+]o-dependent basal currents at −70 mV
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 14 Post hoc testing of [Ca2+]o-dependent basal currents at −70 mV
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 15 [Ca2+]o-dependent depolarization
View this table:
  • View inline
  • View popup
  • Download powerpoint
Supplementary Table 16 Post hoc testing of blocker sensitive fractions of the [Ca2+]o-dependent depolarization

Acknowledgements

This work was supported by grants awarded by U.S. Department of Veterans Affairs (BX002547) and NIGMS (GM134110) to SMS. We thank Dr Wenyan Chen for performing the experiments on potassium channel currents and Dr Glynis Mattheisen, Dr Brian Jones, Ms Natasha Baas-Thomas, and Ms Maya Feldthouse for helpful discussion and comments on the manuscript. Thanks to Drs Chris Harrington and Brittany Daughtry of the OHSU Gene Profiling Shared Resource which performed RNA isolation, quality assessments, and qPCR assays. The authors declare no competing financial interests. The contents do not represent the views of the U.S. Department of Veterans Affairs or the United States Government.

Footnotes

  • Author order corrected

References

  1. ↵
    Al-Sayed MD, Al-Zaidan H, Albakheet A, Hakami H, Kenana R, Al-Yafee Y, Al-Dosary M, Qari A, Al-Sheddi T, Al-Muheiza M, Al-Qubbaj W, Lakmache Y, Al-Hindi H, Ghaziuddin M, Colak D, Kaya N (2013) Mutations in NALCN cause an autosomal-recessive syndrome with severe hypotonia, speech impairment, and cognitive delay. American journal of human genetics 93:721–726.
    OpenUrlCrossRefPubMed
  2. ↵
    Almog M, Barkai T, Lampert A, Korngreen A (2018) Voltage-Gated Sodium Channels in Neocortical Pyramidal Neurons Display Cole-Moore Activation Kinetics. Front Cell Neurosci 12:187.
    OpenUrl
  3. ↵
    Anderson D, Engbers JD, Heath NC, Bartoletti TM, Mehaffey WH, Zamponi GW, Turner RW (2013) The Cav3-Kv4 complex acts as a calcium sensor to maintain inhibitory charge transfer during extracellular calcium fluctuations. The Journal of neuroscience : the official journal of the Society for Neuroscience 33:7811–7824.
    OpenUrlAbstract/FREE Full Text
  4. ↵
    Armstrong CM, Cota G (1991) Calcium ion as a cofactor in Na channel gating. Proceedings of the National Academy of Sciences of the United States of America 88:6528–6531.
    OpenUrlAbstract/FREE Full Text
  5. ↵
    Ayata C, Lauritzen M (2015) Spreading Depression, Spreading Depolarizations, and the Cerebral Vasculature. Physiological reviews 95:953–993.
    OpenUrlCrossRefPubMed
  6. ↵
    Bean BP (2007) The action potential in mammalian central neurons. Nature Reviews Neuroscience 8:451–465.
    OpenUrlCrossRefPubMedWeb of Science
  7. ↵
    Bourque DK, Dyment DA, MacLusky I, Kernohan KD, McMillan HJ (2018) Periodic breathing in patients with NALCN mutations. J Hum Genet 63:1093–1096.
    OpenUrl
  8. ↵
    Campbell DT, Hille B (1976) Kinetic and pharmacological properties of the sodium channel of frog skeletal muscle. J Gen Physiol 67:309–323.
    OpenUrlAbstract/FREE Full Text
  9. ↵
    Cantrell AR, Ma JY, Scheuer T, Catterall WA (1996) Muscarinic modulation of sodium current by activation of protein kinase C in rat hippocampal neurons. Neuron 16:1019–1026.
    OpenUrlCrossRefPubMedWeb of Science
  10. ↵
    Chadda KR, Jeevaratnam K, Lei M, Huang CLH (2017) Sodium channel biophysics, late sodium current and genetic arrhythmic syndromes. Pflugers Archiv : European journal of physiology 469:629–641.
    OpenUrl
  11. ↵
    Chang W, Tu C, Chen TH, Bikle D, Shoback D (2008) The extracellular calcium-sensing receptor (CaSR) is a critical modulator of skeletal development. Science signaling 1:ra1.
    OpenUrlAbstract/FREE Full Text
  12. ↵
    Chen W, Bergsman JB, Wang X, Gilkey G, Pierpoint CR, Daniel EA, Awumey EM, Dauban P, Dodd RH, Ruat M, Smith SM (2010) Presynaptic external calcium signaling involves the calcium-sensing receptor in neocortical nerve terminals. PloS one 5:e8563.
    OpenUrlCrossRefPubMed
  13. ↵
    Elinder F, Arhem P (1994) Effects of gadolinium on ion channels in the myelinated axon of Xenopus laevis: four sites of action. Biophysical Journal 67:71–83.
    OpenUrlCrossRefPubMedWeb of Science
  14. ↵
    Flourakis M, Kula-Eversole E, Hutchison AL, Han TH, Aranda K, Moose DL, White KP, Dinner AR, Lear BC, Ren D, Diekman CO, Raman IM, Allada R (2015) A Conserved Bicycle Model for Circadian Clock Control of Membrane Excitability. Cell 162:836–848.
    OpenUrlCrossRefPubMed
  15. ↵
    Frankenhaeuser B (1957) The effect of calcium on the myelinated nerve fibre. J Physiol 137:245–260.
    OpenUrlCrossRefPubMed
  16. ↵
    Frankenhaeuser B, Hodgkin AL (1957) The action of calcium on the electrical properties of squid axons. J Physiol 137:218–244.
    OpenUrlCrossRefPubMedWeb of Science
  17. ↵
    Gorelova N, Seamans JK (2015) Cell-attached single-channel recordings in intact prefrontal cortex pyramidal neurons reveal compartmentalized D1/D5 receptor modulation of the persistent sodium current. Front Neural Circuits 9:4.
    OpenUrl
  18. ↵
    Hannan FM, Kallay E, Chang W, Brandi ML, Thakker RV (2018) The calcium-sensing receptor in physiology and in calcitropic and noncalcitropic diseases. Nat Rev Endocrinol 15:33–51.
    OpenUrlPubMed
  19. ↵
    Harnett MT, Magee JC, Williams SR (2015) Distribution and function of HCN channels in the apical dendritic tuft of neocortical pyramidal neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience 35:1024–1037.
    OpenUrlAbstract/FREE Full Text
  20. ↵
    Hess P, Lansman JB, Tsien RW (1986) Calcium channel selectivity for divalent and monovalent cations. Voltage and concentration dependence of single channel current in ventricular heart cells. Journal of General Physiology 88:293–319.
    OpenUrlAbstract/FREE Full Text
  21. ↵
    Hille B (1968) Charges and Potentials at the Nerve Surface. Divalent ions and pH. Journal of General Physiology 51:221–236.
    OpenUrlAbstract/FREE Full Text
  22. ↵
    Hille B (2001) Ion channels of excitable membranes, 3rd Edition. Sunderland, Mass.: Sinauer.
  23. ↵
    Hu W, Bean BP (2018) Differential Control of Axonal and Somatic Resting Potential by Voltage-Dependent Conductances in Cortical Layer 5 Pyramidal Neurons. Neuron 97:1315–1326.e1313.
    OpenUrlCrossRefPubMed
  24. ↵
    Isaev D, Ivanchick G, Khmyz V, Isaeva E, Savrasova A, Krishtal O, Holmes GL, Maximyuk O (2012) Surface charge impact in low-magnesium model of seizure in rat hippocampus. Journal of Neurophysiology 107:417–423.
    OpenUrlCrossRefPubMedWeb of Science
  25. ↵
    Jackman SL, Regehr WG (2017) The Mechanisms and Functions of Synaptic Facilitation. Neuron 94:447–464.
    OpenUrlCrossRef
  26. ↵
    Jun K, Piedras-Rentería ES, Smith SM, Wheeler DB, Lee SB, Lee TG, Chin H, Adams ME, Scheller RH, Tsien RW, Shin H-S (1999) Ablation of P/Q-type Ca2+ channel currents, altered synaptic transmission, and progressive ataxia in mice lacking the α1A-subunit. Proceedings of the National Academy of Sciences 96:15245–15250.
    OpenUrlAbstract/FREE Full Text
  27. ↵
    Kim JY, Kim N, Yenari MA, Chang W (2013) Hypothermia and pharmacological regimens that prevent overexpression and overactivity of the extracellular calcium-sensing receptor protect neurons against traumatic brain injury. J Neurotrauma 30:1170–1176.
    OpenUrl
  28. ↵
    Kubo Y, Miyashita T, Murata Y (1998) Structural basis for a Ca2+-sensing function of the metabotropic glutamate receptors. Science 279:1722–1725.
    OpenUrlAbstract/FREE Full Text
  29. ↵
    Kuptanon C, Srichomthong C, Ittiwut C, Wechapinan T, Sri-Udomkajorn S, Iamopas O, Phokaew C, Suphapeetiporn K, Shotelersuk V (2019) Whole exome sequencing revealed mutations in FBXL4, UNC80, and ADK in Thai patients with severe intellectual disabilities. Gene 696:21–27.
    OpenUrl
  30. ↵
    Lear BC, Darrah EJ, Aldrich BT, Gebre S, Scott RL, Nash HA, Allada R (2013) UNC79 and UNC80, putative auxiliary subunits of the NARROW ABDOMEN ion channel, are indispensable for robust circadian locomotor rhythms in Drosophila. PloS one 8:e78147.
    OpenUrlCrossRefPubMed
  31. ↵
    Li G-R, Baumgarten CM (2001) Modulation of cardiac Na+ current by gadolinium, a blocker of stretch-induced arrhythmias. American Journal of Physiology-Heart and Circulatory Physiology 280:H272–H279.
    OpenUrlPubMedWeb of Science
  32. ↵
    Lu B, Su Y, Das S, Liu J, Xia J, Ren D (2007) The neuronal channel NALCN contributes resting sodium permeability and is required for normal respiratory rhythm. Cell 129:371–383.
    OpenUrlCrossRefPubMedWeb of Science
  33. ↵
    Lu B, Zhang Q, Wang H, Wang Y, Nakayama M, Ren D (2010) Extracellular calcium controls background current and neuronal excitability via an UNC79-UNC80-NALCN cation channel complex. Neuron 68:488–499.
    OpenUrlCrossRefPubMedWeb of Science
  34. ↵
    Lu B, Su Y, Das S, Wang H, Wang Y, Liu J, Ren D (2009) Peptide neurotransmitters activate a cation channel complex of NALCN and UNC-80. Nature 457:741–744.
    OpenUrlCrossRefPubMedWeb of Science
  35. ↵
    Ma H, Groth RD, Cohen SM, Emery JF, Li B, Hoedt E, Zhang G, Neubert TA, Tsien RW (2014) gammaCaMKII shuttles Ca(2)(+)/CaM to the nucleus to trigger CREB phosphorylation and gene expression. Cell 159:281–294.
    OpenUrlCrossRefPubMed
  36. ↵
    Ma Z, Siebert AP, Cheung KH, Lee RJ, Johnson B, Cohen AS, Vingtdeux V, Marambaud P, Foskett JK (2012) Calcium homeostasis modulator 1 (CALHM1) is the pore-forming subunit of an ion channel that mediates extracellular Ca2+ regulation of neuronal excitability. Proceedings of the National Academy of Sciences of the United States of America 109:E1963–1971.
    OpenUrlAbstract/FREE Full Text
  37. ↵
    Mattheisen GB, Tsintsadze T, Smith SM (2018) Strong G-Protein-Mediated Inhibition of Sodium Channels. Cell Rep 23:2770–2781.
    OpenUrl
  38. ↵
    Montero-Pau J, Gómez A, Muñoz J (2008) Application of an inexpensive and high-throughput genomic DNA extraction method for the molecular ecology of zooplanktonic diapausing eggs. Limnology and Oceanography: Methods 6:218–222.
    OpenUrlCrossRef
  39. ↵
    Nanou E, Catterall WA (2018) Calcium Channels, Synaptic Plasticity, and Neuropsychiatric Disease. Neuron 98:466–481.
    OpenUrl
  40. ↵
    Neher E, Sakaba T (2008) Multiple roles of calcium ions in the regulation of neurotransmitter release. Neuron 59:861–872.
    OpenUrlCrossRefPubMedWeb of Science
  41. ↵
    Nicholson C, ten Bruggencate G, Stockle H, Steinberg R (1978) Calcium and potassium changes in extracellular microenvironment of cat cerebellar cortex. J Neurophysiol 41:1026–1039.
    OpenUrlPubMedWeb of Science
  42. ↵
    Ohta K, Graf R, Rosner G, Heiss WD (1997) Profiles of cortical tissue depolarization in cat focal cerebral ischemia in relation to calcium ion homeostasis and nitric oxide production. J Cereb Blood Flow Metab 17:1170–1181.
    OpenUrlCrossRefPubMedWeb of Science
  43. ↵
    Perez Y, Kadir R, Volodarsky M, Noyman I, Flusser H, Shorer Z, Gradstein L, Birnbaum RY, Birk OS (2016) UNC80 mutation causes a syndrome of hypotonia, severe intellectual disability, dyskinesia and dysmorphism, similar to that caused by mutations in its interacting cation channel NALCN. Journal of medical genetics 53:397–402.
    OpenUrlAbstract/FREE Full Text
  44. ↵
    Philippart F, Khaliq ZM (2018) Gi/o protein-coupled receptors in dopamine neurons inhibit the sodium leak channel NALCN. Elife 7.
  45. ↵
    Phillips CG, Harnett MT, Chen W, Smith SM (2008) Calcium-sensing receptor activation depresses synaptic transmission. The Journal of neuroscience : the official journal of the Society for Neuroscience 28:12062–12070.
    OpenUrlAbstract/FREE Full Text
  46. ↵
    Pietrobon D, Moskowitz MA (2014) Chaos and commotion in the wake of cortical spreading depression and spreading depolarizations. Nat Rev Neurosci 15:379–393.
    OpenUrlCrossRefPubMed
  47. ↵
    Qiu L, Rivera-Perez JA, Xu Z (2011) A non-specific effect associated with conditional transgene expression based on Cre-loxP strategy in mice. PloS one 6:e18778.
    OpenUrlCrossRefPubMed
  48. ↵
    Sather W, Dieudonne S, MacDonald JF, Ascher P (1992) Activation and desensitization of N-methyl-D-aspartate receptors in nucleated outside-out patches from mouse neurones. J Physiol 450:643–672.
    OpenUrlCrossRefPubMedWeb of Science
  49. ↵
    Smith SM, Bergsman JB, Harata NC, Scheller RH, Tsien RW (2004) Recordings from single neocortical nerve terminals reveal a nonselective cation channel activated by decreases in extracellular calcium. Neuron 41:243–256.
    OpenUrlCrossRefPubMedWeb of Science
  50. ↵
    Sokolov S, Scheuer T, Catterall WA (2007) Gating pore current in an inherited ion channelopathy. Nature 446:76–78.
    OpenUrlCrossRefPubMedWeb of Science
  51. ↵
    Stray-Pedersen A et al. (2016) Biallelic Mutations in UNC80 Cause Persistent Hypotonia, Encephalopathy, Growth Retardation, and Severe Intellectual Disability. American journal of human genetics 98:202–209.
    OpenUrlCrossRef
  52. ↵
    Sun X, Tang L, Winesett S, Chang W, Cheng SX (2018) Calcimimetic R568 inhibits tetrodotoxin-sensitive colonic electrolyte secretion and reduces c-fos expression in myenteric neurons. Life Sci 194:49–58.
    OpenUrl
  53. ↵
    Swayne LA, Mezghrani A, Varrault A, Chemin J, Bertrand G, Dalle S, Bourinet E, Lory P, Miller RJ, Nargeot J, Monteil A (2009) The NALCN ion channel is activated by M3 muscarinic receptors in a pancreatic beta-cell line. EMBO reports 10:873–880.
    OpenUrlAbstract/FREE Full Text
  54. ↵
    Tabata T, Kano M (2004) Calcium dependence of native metabotropic glutamate receptor signaling in central neurons. Mol Neurobiol 29:261–270.
    OpenUrlCrossRefPubMedWeb of Science
  55. ↵
    Taddese A, Bean BP (2002) Subthreshold sodium current from rapidly inactivating sodium channels drives spontaneous firing of tuberomammillary neurons. Neuron 33:587–600.
    OpenUrlCrossRefPubMedWeb of Science
  56. ↵
    Talley EM, Solorzano G, Lei Q, Kim D, Bayliss DA (2001) Cns distribution of members of the two-pore-domain (KCNK) potassium channel family. The Journal of neuroscience : the official journal of the Society for Neuroscience 21:7491–7505.
    OpenUrlAbstract/FREE Full Text
  57. ↵
    Tavalin SJ, Ellis EF, Satin LS (1997) Inhibition of the Electrogenic Na Pump Underlies Delayed Depolarization of Cortical Neurons After Mechanical Injury or Glutamate. Journal of Neurophysiology 77:632–638.
    OpenUrlPubMedWeb of Science
  58. ↵
    Titley HK, Kislin M, Simmons DH, Wang SS, Hansel C (2019) Complex spike clusters and false-positive rejection in a cerebellar supervised learning rule. J Physiol 597:4387–4406.
    OpenUrl
  59. ↵
    Vyleta NP, Smith SM (2011) Spontaneous glutamate release is independent of calcium influx and tonically activated by the calcium-sensing receptor. The Journal of neuroscience : the official journal of the Society for Neuroscience 31:4593–4606.
    OpenUrlAbstract/FREE Full Text
  60. ↵
    Weber AM, Wong FK, Tufford AR, Schlichter LC, Matveev V, Stanley EF (2010) N-type Ca2+ channels carry the largest current: implications for nanodomains and transmitter release. Nat Neurosci 13:1348–1350.
    OpenUrlCrossRefPubMed
  61. ↵
    Weidmann S (1955) Effects of calcium ions and local anaesthetics on electrical properties of Purkinje fibres. The Journal of Physiology 129:568–582.
    OpenUrlCrossRefPubMedWeb of Science
  62. ↵
    Zhang F, Wang Y, Liu Y, Han H, Zhang D, Fan X, Du X, Gamper N, Zhang H (2019) Transcriptional Regulation of Voltage-Gated Sodium Channels Contributes to GM-CSF-Induced Pain. The Journal of neuroscience : the official journal of the Society for Neuroscience 39:5222–5233.
    OpenUrlAbstract/FREE Full Text
Back to top
PreviousNext
Posted March 04, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
CaSR modulates sodium channel-mediated Ca2+-dependent excitability
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
CaSR modulates sodium channel-mediated Ca2+-dependent excitability
Briana J. Martiszus, Timur Tsintsadze, Wenhan Chang, Stephen M. Smith
bioRxiv 2021.03.03.433701; doi: https://doi.org/10.1101/2021.03.03.433701
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
CaSR modulates sodium channel-mediated Ca2+-dependent excitability
Briana J. Martiszus, Timur Tsintsadze, Wenhan Chang, Stephen M. Smith
bioRxiv 2021.03.03.433701; doi: https://doi.org/10.1101/2021.03.03.433701

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4227)
  • Biochemistry (9107)
  • Bioengineering (6751)
  • Bioinformatics (23944)
  • Biophysics (12089)
  • Cancer Biology (9495)
  • Cell Biology (13740)
  • Clinical Trials (138)
  • Developmental Biology (7616)
  • Ecology (11661)
  • Epidemiology (2066)
  • Evolutionary Biology (15479)
  • Genetics (10617)
  • Genomics (14296)
  • Immunology (9462)
  • Microbiology (22792)
  • Molecular Biology (9078)
  • Neuroscience (48888)
  • Paleontology (355)
  • Pathology (1479)
  • Pharmacology and Toxicology (2565)
  • Physiology (3823)
  • Plant Biology (8308)
  • Scientific Communication and Education (1467)
  • Synthetic Biology (2290)
  • Systems Biology (6172)
  • Zoology (1297)