Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Enabling single-molecule localization microscopy in turbid food emulsions

View ORCID ProfileAbbas Jabermoradi, View ORCID ProfileSuyeon Yang, View ORCID ProfileMartijn Gobes, View ORCID ProfileJohn P.M. van Duynhoven, View ORCID ProfileJohannes Hohlbein
doi: https://doi.org/10.1101/2021.03.03.433739
Abbas Jabermoradi
aLaboratory of Biophysics, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Abbas Jabermoradi
Suyeon Yang
aLaboratory of Biophysics, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Suyeon Yang
Martijn Gobes
aLaboratory of Biophysics, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Martijn Gobes
John P.M. van Duynhoven
aLaboratory of Biophysics, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands
bUnilever Global Foods Innovation Centre, Wageningen, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for John P.M. van Duynhoven
  • For correspondence: john.vanduynhoven@wur.nl johannes.hohlbein@wur.nl
Johannes Hohlbein
aLaboratory of Biophysics, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands
cMicrospectroscopy Research Facility, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Johannes Hohlbein
  • For correspondence: john.vanduynhoven@wur.nl johannes.hohlbein@wur.nl
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Turbidity poses a major challenge for the microscopic characterization of many food systems. In these systems, local mismatches in refractive indices can cause reflection, absorption and scattering of incoming as well as outgoing light leading to significant image deterioration along sample depth. To mitigate the issue of turbidity and to increase the achievable optical resolution, we combined adaptive optics (AO) with single-molecule localization microscopy (SMLM). Building on our previously published open hardware microscopy framework, the miCube, we first added a deformable mirror to the detection path. This element enables both the compensation of aberrations directly from single-molecule data and, by further modulating the emission wavefront, the introduction of various point spread functions (PSFs) to enable SMLM in three dimensions. We further added a top hat beam shaper to the excitation path to obtain an even illumination profile across the field of view (FOV). As a model system for a non-transparent food colloid in which imaging in depth is challenging, we designed an oil-in-water emulsion in which phosvitin, a ferric ion binding protein present in from egg yolk, resides at the oil water interface. We targeted phosvitin with fluorescently labelled primary antibodies and used PSF engineering to obtain 2D and 3D images of phosvitin covered oil droplets with sub 100 nm resolution. Droplets with radii as low as 200 nm can be discerned, which is beyond the range of conventional confocal light microscopy. Our data indicated that in the model emulsion phosvitin is homogeneously distributed at the oil-water interface. With the possibility to obtain super-resolved images in depth of nontransparent colloids, our work paves the way for localizing biomacromolecules at colloidal interfaces in heterogeneous food emulsions.

Competing Interest Statement

J.P.M.v.D. is employed by a company that manufactures and markets mayonnaise.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
Back to top
PreviousNext
Posted March 03, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Enabling single-molecule localization microscopy in turbid food emulsions
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Enabling single-molecule localization microscopy in turbid food emulsions
Abbas Jabermoradi, Suyeon Yang, Martijn Gobes, John P.M. van Duynhoven, Johannes Hohlbein
bioRxiv 2021.03.03.433739; doi: https://doi.org/10.1101/2021.03.03.433739
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Enabling single-molecule localization microscopy in turbid food emulsions
Abbas Jabermoradi, Suyeon Yang, Martijn Gobes, John P.M. van Duynhoven, Johannes Hohlbein
bioRxiv 2021.03.03.433739; doi: https://doi.org/10.1101/2021.03.03.433739

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biophysics
Subject Areas
All Articles
  • Animal Behavior and Cognition (2646)
  • Biochemistry (5269)
  • Bioengineering (3678)
  • Bioinformatics (15796)
  • Biophysics (7257)
  • Cancer Biology (5629)
  • Cell Biology (8099)
  • Clinical Trials (138)
  • Developmental Biology (4768)
  • Ecology (7518)
  • Epidemiology (2059)
  • Evolutionary Biology (10578)
  • Genetics (7733)
  • Genomics (10137)
  • Immunology (5194)
  • Microbiology (13915)
  • Molecular Biology (5387)
  • Neuroscience (30785)
  • Paleontology (215)
  • Pathology (879)
  • Pharmacology and Toxicology (1525)
  • Physiology (2254)
  • Plant Biology (5024)
  • Scientific Communication and Education (1041)
  • Synthetic Biology (1388)
  • Systems Biology (4148)
  • Zoology (812)