New Results
A genetically encoded fluorescent biosensor for extracellular L-lactate
Yusuke Nasu, Ciaran Murphy-Royal, Yurong Wen, Jordan Haidey, M. Rosana S. Molina, Abhi Aggarwal, Shuce Zhang, Yuki Kamijo, Marie-Eve Paquet, Kaspar Podgorski, Mikhail Drobizhev, Jaideep S. Bains, M. Joanne Lemieux, Grant R. Gordon, Robert E. Campbell
doi: https://doi.org/10.1101/2021.03.05.434048
Yusuke Nasu
1Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
Ciaran Murphy-Royal
2Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB T2N 4N1, Canada
3Centre Hospitalier de l’Université de Montréal, Department of Neuroscience, Faculty of Medicine, University of Montreal, Montreal, QC H2X 0A9, Canada
Yurong Wen
4Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada
5Department of Talent Highland, The First Affiliated Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi 710061, China
Jordan Haidey
2Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB T2N 4N1, Canada
M. Rosana S. Molina
6Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA
Abhi Aggarwal
7Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Shuce Zhang
8Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
Yuki Kamijo
1Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
Marie-Eve Paquet
9Department of Biochemistry, Microbiology and Bioinformatics, University of Laval, Québec, QC G1J 2G3, Canada
Kaspar Podgorski
7Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Mikhail Drobizhev
6Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA
Jaideep S. Bains
2Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB T2N 4N1, Canada
M. Joanne Lemieux
4Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada
Grant R. Gordon
2Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB T2N 4N1, Canada
Robert E. Campbell
1Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
8Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
Abstract
To enable investigations of the emerging roles of cell-to-cell shuttling of L-lactate, we have developed an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. We demonstrate that this biosensor, designated eLACCO1.1, enables minimally invasive cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.
Competing Interest Statement
The authors have declared no competing interest.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Posted March 06, 2021.
A genetically encoded fluorescent biosensor for extracellular L-lactate
Yusuke Nasu, Ciaran Murphy-Royal, Yurong Wen, Jordan Haidey, M. Rosana S. Molina, Abhi Aggarwal, Shuce Zhang, Yuki Kamijo, Marie-Eve Paquet, Kaspar Podgorski, Mikhail Drobizhev, Jaideep S. Bains, M. Joanne Lemieux, Grant R. Gordon, Robert E. Campbell
bioRxiv 2021.03.05.434048; doi: https://doi.org/10.1101/2021.03.05.434048
A genetically encoded fluorescent biosensor for extracellular L-lactate
Yusuke Nasu, Ciaran Murphy-Royal, Yurong Wen, Jordan Haidey, M. Rosana S. Molina, Abhi Aggarwal, Shuce Zhang, Yuki Kamijo, Marie-Eve Paquet, Kaspar Podgorski, Mikhail Drobizhev, Jaideep S. Bains, M. Joanne Lemieux, Grant R. Gordon, Robert E. Campbell
bioRxiv 2021.03.05.434048; doi: https://doi.org/10.1101/2021.03.05.434048
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