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Eliminating Mosquitoes with Precision Guided Sterile Males

Ming Li, Ting Yang, Michelle Bui, Stephanie Gamez, Tyler Wise, Nikolay P. Kandul, Junru Liu, Lenissa Alcantara, Haena Lee, Jyotheeswara R. Edula, Robyn Raban, Yinpeng Zhan, Yijin Wang, Nick DeBeaubien, Jieyan Chen, Hector M. Sanchez C., Jared B. Bennett, Igor Antoshechkin, Craig Montell, John M. Marshall, View ORCID ProfileOmar S. Akbari
doi: https://doi.org/10.1101/2021.03.05.434167
Ming Li
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA
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Ting Yang
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA
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Michelle Bui
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA
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Stephanie Gamez
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA
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Tyler Wise
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA
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Nikolay P. Kandul
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA
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Junru Liu
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA
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Lenissa Alcantara
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA
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Haena Lee
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA
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Jyotheeswara R. Edula
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA
2Tata Institute for Genetics and Society, La Jolla, CA 92093, USA
3Tata Institute for Genetics and Society (TIGS), TIGS Center at inStem, GKVK Campus, Bangalore, 560065, India
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Robyn Raban
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA
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Yinpeng Zhan
4Department of Molecular, Cellular, and Developmental Biology and the Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
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Yijin Wang
4Department of Molecular, Cellular, and Developmental Biology and the Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
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Nick DeBeaubien
4Department of Molecular, Cellular, and Developmental Biology and the Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
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Jieyan Chen
4Department of Molecular, Cellular, and Developmental Biology and the Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
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Hector M. Sanchez C.
6Division of Epidemiology & Biostatistics, School of Public Health, University of California, Berkeley, CA 94720, USA
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Jared B. Bennett
5Biophysics Graduate Group, University of California, Berkeley, CA, 94720, USA
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Igor Antoshechkin
7Division of Biology and Biological Engineering (BBE), California Institute of Technology, Pasadena, CA 91125, USA
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Craig Montell
4Department of Molecular, Cellular, and Developmental Biology and the Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
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John M. Marshall
6Division of Epidemiology & Biostatistics, School of Public Health, University of California, Berkeley, CA 94720, USA
8Innovative Genomics Institute, Berkeley, CA 94720, USA
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Omar S. Akbari
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA
2Tata Institute for Genetics and Society, La Jolla, CA 92093, USA
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  • ORCID record for Omar S. Akbari
  • For correspondence: oakbari@ucsd.edu
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Abstract

The mosquito Aedes aegypti is the principal vector for arboviruses including dengue/yellow fever, chikungunya, and zika, infecting hundreds of millions of people annually. Unfortunately, traditional control methodologies are insufficient, so innovative control methods are needed. To complement existing measures, here we develop a molecular genetic control system termed precision guided sterile insect technique (pgSIT) in Aedes aegypti. PgSIT uses a simple CRISPR-based approach to generate sterile males that are deployable at any life stage. Supported by mathematical models, we empirically demonstrate that released pgSIT males can compete, suppress, and eliminate mosquitoes in multigenerational population cages. This platform technology could be used in the field, and adapted to many vectors, for controlling wild populations to curtail disease in a safe, confinable, and reversible manner.

Competing Interest Statement

O.S.A is a founder of Agragene Inc has equity interest and serves on the companys Scientific Advisory Board. The terms of this arrangement have been reviewed and approved by the University of California San Diego in accordance with its conflict of interest policies. All other authors declare no competing interests.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted March 06, 2021.
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Eliminating Mosquitoes with Precision Guided Sterile Males
Ming Li, Ting Yang, Michelle Bui, Stephanie Gamez, Tyler Wise, Nikolay P. Kandul, Junru Liu, Lenissa Alcantara, Haena Lee, Jyotheeswara R. Edula, Robyn Raban, Yinpeng Zhan, Yijin Wang, Nick DeBeaubien, Jieyan Chen, Hector M. Sanchez C., Jared B. Bennett, Igor Antoshechkin, Craig Montell, John M. Marshall, Omar S. Akbari
bioRxiv 2021.03.05.434167; doi: https://doi.org/10.1101/2021.03.05.434167
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Eliminating Mosquitoes with Precision Guided Sterile Males
Ming Li, Ting Yang, Michelle Bui, Stephanie Gamez, Tyler Wise, Nikolay P. Kandul, Junru Liu, Lenissa Alcantara, Haena Lee, Jyotheeswara R. Edula, Robyn Raban, Yinpeng Zhan, Yijin Wang, Nick DeBeaubien, Jieyan Chen, Hector M. Sanchez C., Jared B. Bennett, Igor Antoshechkin, Craig Montell, John M. Marshall, Omar S. Akbari
bioRxiv 2021.03.05.434167; doi: https://doi.org/10.1101/2021.03.05.434167

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