Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Structural Analysis of Spike Protein Mutations in the SARS-CoV-2 P.3 Variant

Neil Andrew D. Bascos, Denise Mirano-Bascos, Cynthia P. Saloma
doi: https://doi.org/10.1101/2021.03.06.434059
Neil Andrew D. Bascos
1Philippine Genome Center, University of the Philippines, Diliman, Quezon City 1101, Philippines
2National Institute of Molecular Biology and Biotechnology, University of the Philippines Diliman, Quezon City 1101, Philippines
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: neilandrew.bascos@mbb.upd.edu.ph
Denise Mirano-Bascos
2National Institute of Molecular Biology and Biotechnology, University of the Philippines Diliman, Quezon City 1101, Philippines
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cynthia P. Saloma
1Philippine Genome Center, University of the Philippines, Diliman, Quezon City 1101, Philippines
2National Institute of Molecular Biology and Biotechnology, University of the Philippines Diliman, Quezon City 1101, Philippines
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Data/Code
  • Preview PDF
Loading

ABSTRACT

A SARS-CoV-2 lineage designated as P.3 with multiple signature mutations in the Spike protein region was recently reported with cases from the Central Visayas Region of the Philippines. Whole genome sequencing revealed that the 33 samples under this lineage all contain the E484K, N501Y, and P681H Spike mutations previously found in variants of concern (VOC) such as the B.1.351, the P.1 and B.1.1.7 variants first reported in South Africa, Brazil, and the United Kingdom, respectively. The possible implications of the mutations found in the Spike protein of P.3 were analyzed for their potential effects on structure, stability, and molecular surface character. The analysis suggests that these mutations could significantly impact the possible interactions of the Spike protein with the ACE2 receptor and neutralizing antibodies, and warrants further clinical investigation. Some of the mutations affecting the N and C terminal domains may have effects on Spike monomer and trimer stability. This report provides insights on relevant targets for the design of future diagnostics, therapeutics and vaccines against the evolving SARS-CoV-2 variants in the Philippines.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • This version of the manuscript was revised to update the WHO designation of the variant as P.3, to revise the manuscript title, and to add figures and figure labels to the the document.

  • https://www.epicov.org/epi3/frontend#35ea15

  • https://zhanglab.ccmb.med.umich.edu/COVID-19/

  • https://raptorx.uchicago.edu

  • https://doh.gov.ph/covid19tracker

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted March 22, 2021.
Download PDF
Data/Code
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Structural Analysis of Spike Protein Mutations in the SARS-CoV-2 P.3 Variant
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Structural Analysis of Spike Protein Mutations in the SARS-CoV-2 P.3 Variant
Neil Andrew D. Bascos, Denise Mirano-Bascos, Cynthia P. Saloma
bioRxiv 2021.03.06.434059; doi: https://doi.org/10.1101/2021.03.06.434059
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Structural Analysis of Spike Protein Mutations in the SARS-CoV-2 P.3 Variant
Neil Andrew D. Bascos, Denise Mirano-Bascos, Cynthia P. Saloma
bioRxiv 2021.03.06.434059; doi: https://doi.org/10.1101/2021.03.06.434059

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biophysics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4095)
  • Biochemistry (8786)
  • Bioengineering (6493)
  • Bioinformatics (23386)
  • Biophysics (11766)
  • Cancer Biology (9167)
  • Cell Biology (13290)
  • Clinical Trials (138)
  • Developmental Biology (7422)
  • Ecology (11386)
  • Epidemiology (2066)
  • Evolutionary Biology (15119)
  • Genetics (10413)
  • Genomics (14024)
  • Immunology (9145)
  • Microbiology (22108)
  • Molecular Biology (8793)
  • Neuroscience (47445)
  • Paleontology (350)
  • Pathology (1423)
  • Pharmacology and Toxicology (2483)
  • Physiology (3711)
  • Plant Biology (8063)
  • Scientific Communication and Education (1433)
  • Synthetic Biology (2215)
  • Systems Biology (6021)
  • Zoology (1251)