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SARS-CoV-2 501Y.V2 (B.1.351) elicits cross-reactive neutralizing antibodies

Thandeka Moyo-Gwete, Mashudu Madzivhandila, Zanele Makhado, Frances Ayres, Donald Mhlanga, Brent Oosthuysen, Bronwen E. Lambson, Prudence Kgagudi, Houriiyah Tegally, Arash Iranzadeh, Deelan Doolabh, Lynn Tyers, Lionel R. Chinhoyi, Mathilda Mennen, Sango Skelem, Gert Marais, Constantinos Kurt Wibmer, Jinal N Bhiman, Veronica Ueckermann, Theresa Rossouw, Michael Boswell, View ORCID ProfileTulio de Oliveira, Carolyn Williamson, Wendy A Burgers, Ntobeko Ntusi, Lynn Morris, View ORCID ProfilePenny L Moore
doi: https://doi.org/10.1101/2021.03.06.434193
Thandeka Moyo-Gwete
1National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa
2Antibody Immunity Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
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Mashudu Madzivhandila
1National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa
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Zanele Makhado
1National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa
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Frances Ayres
1National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa
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Donald Mhlanga
1National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa
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Brent Oosthuysen
1National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa
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Bronwen E. Lambson
1National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa
2Antibody Immunity Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
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Prudence Kgagudi
1National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa
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Houriiyah Tegally
3KwaZulu-Natal Research Innovation and Sequencing Platform (KRISP), Department of Laboratory Medicine & Medical Sciences, University of KwaZulu-Natal, Durban, South Africa
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Arash Iranzadeh
4Institute of Infectious Disease and Molecular Medicine, Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa
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Deelan Doolabh
4Institute of Infectious Disease and Molecular Medicine, Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa
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Lynn Tyers
4Institute of Infectious Disease and Molecular Medicine, Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa
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Lionel R. Chinhoyi
5Wellcome Centre for Infectious Diseases Research in Africa, University of Cape Town, Cape Town, South Africa
6Division of Cardiology, Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa
7Hatter Institute for Cardiovascular Research in Africa, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa
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Mathilda Mennen
5Wellcome Centre for Infectious Diseases Research in Africa, University of Cape Town, Cape Town, South Africa
6Division of Cardiology, Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa
7Hatter Institute for Cardiovascular Research in Africa, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa
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Sango Skelem
5Wellcome Centre for Infectious Diseases Research in Africa, University of Cape Town, Cape Town, South Africa
6Division of Cardiology, Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa
7Hatter Institute for Cardiovascular Research in Africa, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa
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Gert Marais
4Institute of Infectious Disease and Molecular Medicine, Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa
8National Health Laboratory Services, Groote Schuur Hospital, Cape Town, South Africa
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Constantinos Kurt Wibmer
1National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa
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Jinal N Bhiman
1National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa
9Department of Virology, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
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Veronica Ueckermann
10Division for Infectious Diseases, Department of Internal Medicine, Steve Biko Academic Hospital and University of Pretoria, Pretoria, South Africa
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Theresa Rossouw
11Department of Immunology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa
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Michael Boswell
10Division for Infectious Diseases, Department of Internal Medicine, Steve Biko Academic Hospital and University of Pretoria, Pretoria, South Africa
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Tulio de Oliveira
3KwaZulu-Natal Research Innovation and Sequencing Platform (KRISP), Department of Laboratory Medicine & Medical Sciences, University of KwaZulu-Natal, Durban, South Africa
12Centre for the AIDS Programme of Research in South Africa (CAPRISA), Durban, South Africa
13Department of Global Health, University of Washington, Seattle, USA
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Carolyn Williamson
4Institute of Infectious Disease and Molecular Medicine, Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa
5Wellcome Centre for Infectious Diseases Research in Africa, University of Cape Town, Cape Town, South Africa
8National Health Laboratory Services, Groote Schuur Hospital, Cape Town, South Africa
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Wendy A Burgers
4Institute of Infectious Disease and Molecular Medicine, Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa
5Wellcome Centre for Infectious Diseases Research in Africa, University of Cape Town, Cape Town, South Africa
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Ntobeko Ntusi
5Wellcome Centre for Infectious Diseases Research in Africa, University of Cape Town, Cape Town, South Africa
6Division of Cardiology, Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa
7Hatter Institute for Cardiovascular Research in Africa, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa
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Lynn Morris
1National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa
2Antibody Immunity Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
12Centre for the AIDS Programme of Research in South Africa (CAPRISA), Durban, South Africa
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Penny L Moore
1National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa
2Antibody Immunity Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
12Centre for the AIDS Programme of Research in South Africa (CAPRISA), Durban, South Africa
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  • ORCID record for Penny L Moore
  • For correspondence: pennym@nicd.ac.za
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Abstract

Neutralization escape by SARS-CoV-2 variants, as has been observed in the 501Y.V2 (B.1.351) variant, has impacted the efficacy of first generation COVID-19 vaccines. Here, the antibody response to the 501Y.V2 variant was examined in a cohort of patients hospitalized with COVID-19 in early 2021 - when over 90% of infections in South Africa were attributed to 501Y.V2. Robust binding and neutralizing antibody titers to the 501Y.V2 variant were detected and these binding antibodies showed high levels of cross-reactivity for the original variant, from the first wave. In contrast to an earlier study where sera from individuals infected with the original variant showed dramatically reduced potency against 501Y.V2, sera from 501Y.V2-infected patients maintained good cross-reactivity against viruses from the first wave. Furthermore, sera from 501Y.V2-infected patients also neutralized the 501Y.V3 (P.1) variant first described in Brazil, and now circulating globally. Collectively these data suggest that the antibody response in patients infected with 501Y.V2 has a broad specificity and that vaccines designed with the 501Y.V2 sequence may elicit more cross-reactive responses.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 11, 2021.
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SARS-CoV-2 501Y.V2 (B.1.351) elicits cross-reactive neutralizing antibodies
Thandeka Moyo-Gwete, Mashudu Madzivhandila, Zanele Makhado, Frances Ayres, Donald Mhlanga, Brent Oosthuysen, Bronwen E. Lambson, Prudence Kgagudi, Houriiyah Tegally, Arash Iranzadeh, Deelan Doolabh, Lynn Tyers, Lionel R. Chinhoyi, Mathilda Mennen, Sango Skelem, Gert Marais, Constantinos Kurt Wibmer, Jinal N Bhiman, Veronica Ueckermann, Theresa Rossouw, Michael Boswell, Tulio de Oliveira, Carolyn Williamson, Wendy A Burgers, Ntobeko Ntusi, Lynn Morris, Penny L Moore
bioRxiv 2021.03.06.434193; doi: https://doi.org/10.1101/2021.03.06.434193
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SARS-CoV-2 501Y.V2 (B.1.351) elicits cross-reactive neutralizing antibodies
Thandeka Moyo-Gwete, Mashudu Madzivhandila, Zanele Makhado, Frances Ayres, Donald Mhlanga, Brent Oosthuysen, Bronwen E. Lambson, Prudence Kgagudi, Houriiyah Tegally, Arash Iranzadeh, Deelan Doolabh, Lynn Tyers, Lionel R. Chinhoyi, Mathilda Mennen, Sango Skelem, Gert Marais, Constantinos Kurt Wibmer, Jinal N Bhiman, Veronica Ueckermann, Theresa Rossouw, Michael Boswell, Tulio de Oliveira, Carolyn Williamson, Wendy A Burgers, Ntobeko Ntusi, Lynn Morris, Penny L Moore
bioRxiv 2021.03.06.434193; doi: https://doi.org/10.1101/2021.03.06.434193

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