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Two different cell-cycle processes determine the timing of cell division in Escherichia coli

View ORCID ProfileAlexandra Colin, View ORCID ProfileGabriele Micali, View ORCID ProfileLouis Faure, View ORCID ProfileMarco Cosentino Lagomarsino, View ORCID ProfileSven van Teeffelen
doi: https://doi.org/10.1101/2021.03.08.434443
Alexandra Colin
1Microbial Morphogenesis and Growth Laboratory, Institut Pasteur, Paris, France
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Gabriele Micali
2Department of Environmental Microbiology, Eawag, Dübendorf, Switzerland
3Department of Environmental Systems Science, ETH Zürich, Zürich Switzerland
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Louis Faure
1Microbial Morphogenesis and Growth Laboratory, Institut Pasteur, Paris, France
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Marco Cosentino Lagomarsino
4IFOM, FIRC Institute of Molecular Oncology, Via Adamello 16, 20139, Milan, Italy
5Physics Department, University of Milan, and I.N.F.N., Via Celoria 16, 20133, Milan, Italy
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  • For correspondence: sven.vanteeffelen@gmail.com marco.cosentino-lagomarsino@ifom.eu
Sven van Teeffelen
6Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Pavillon Roger-Gaudry, C.P. 6128, Succursale Centreville, Montréal, Canada
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  • For correspondence: sven.vanteeffelen@gmail.com marco.cosentino-lagomarsino@ifom.eu
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Abstract

Cells must control the cell cycle to ensure that key processes are brought to completion. In Escherichia coli, it is controversial whether cell division is tied to chromosome replication or to a replication-independent inter-division process. A recent model suggests instead that both processes may limit cell division with comparable odds in single cells. Here, we tested this possibility experimentally by monitoring single-cell division and replication over multiple generations at slow growth. We then perturbed cell width, causing an increase of the time between replication termination and division. As a consequence, replication became decreasingly limiting 21 for cell division, while correlations between birth and division and between subsequent replication-initiation events were maintained. Our experiments support the hypothesis that both chromosome replication and a replication-independent inter-division process can limit cell division: the two processes have balanced contributions in non-perturbed cells, while our width perturbations increase the odds of the replication-independent process being limiting.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵§ Department of Molecular Neurosciences, Center for Brain Research, Medical University Vienna, 1090 Vienna, Austria

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted March 09, 2021.
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Two different cell-cycle processes determine the timing of cell division in Escherichia coli
Alexandra Colin, Gabriele Micali, Louis Faure, Marco Cosentino Lagomarsino, Sven van Teeffelen
bioRxiv 2021.03.08.434443; doi: https://doi.org/10.1101/2021.03.08.434443
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Two different cell-cycle processes determine the timing of cell division in Escherichia coli
Alexandra Colin, Gabriele Micali, Louis Faure, Marco Cosentino Lagomarsino, Sven van Teeffelen
bioRxiv 2021.03.08.434443; doi: https://doi.org/10.1101/2021.03.08.434443

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