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Minimizing spectral overlap in multicolor flow cytometry experiments

View ORCID ProfileLars Rønn Olsen, View ORCID ProfileAlfredo Benso, Gianfranco Politano, View ORCID ProfileMike Bogetofte Barnkob
doi: https://doi.org/10.1101/2021.03.17.435861
Lars Rønn Olsen
1Section for Bioinformatics, Department of Health Technology, Technical University of Denmark
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  • For correspondence: lronn@dtu.dk
Alfredo Benso
2Computer and Control Engineering Department, Politecnico di Torino, Italy
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Gianfranco Politano
2Computer and Control Engineering Department, Politecnico di Torino, Italy
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Mike Bogetofte Barnkob
3Centre for Cellular Immunotherapy of Haematological Cancer Odense (CITCO), Department of Clinical Immunology, Odense University Hospital, University of Southern Denmark, Odense, Denmark
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Abstract

Selecting fluorochromes for polychromatic panels for flow cytometry is complex and time-consuming. Poorly designed panels can result in overlap between the emission spectra of the different fluorochromes, making their signals difficult to separate. While assessing all possible panels is simple to do programmatically, the combinatorial complexity of most real world problems renders brute force computation impractical. Here we present a novel complexity optimization algorithm for fast design of minimal spectral overlap fluorochrome combinations. To aid researchers in designing fluorochrome panels we implemented the algorithm in a web server, Spectracular, that also considers instrument laser configuration and allows users to define various constraints related to antibody-fluorochrome availability and marker co-expression.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Contact information, Section for Bioinformatics, Department of Health Technology, Technical University of Denmark, Kemitorvet, Building 204, 2800 Kongens Lyngby, Denmark, lronn{at}dtu.dk

  • Funding, This work was funded by the Independent Research Fund Denmark (grant 8048-00078B to LRO).

  • https://biosurf.org/spectracular.html

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted November 06, 2021.
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Minimizing spectral overlap in multicolor flow cytometry experiments
Lars Rønn Olsen, Alfredo Benso, Gianfranco Politano, Mike Bogetofte Barnkob
bioRxiv 2021.03.17.435861; doi: https://doi.org/10.1101/2021.03.17.435861
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Minimizing spectral overlap in multicolor flow cytometry experiments
Lars Rønn Olsen, Alfredo Benso, Gianfranco Politano, Mike Bogetofte Barnkob
bioRxiv 2021.03.17.435861; doi: https://doi.org/10.1101/2021.03.17.435861

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