Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Neurexin and Frizzled signaling intercept axonal-transport at microtubule minus-ends to control synapse formation

Santiago Balseiro-Gómez, Yang Yue, Lin Shao, Selim Ҫetinkaya, Caroline Kuzoian, Kristen J Verhey, Shaul Yogev
doi: https://doi.org/10.1101/2021.03.22.436477
Santiago Balseiro-Gómez
1Department of Neuroscience, Yale School of Medicine, 295 Congress Ave, New Haven, CT 06510
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yang Yue
2Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lin Shao
1Department of Neuroscience, Yale School of Medicine, 295 Congress Ave, New Haven, CT 06510
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Selim Ҫetinkaya
1Department of Neuroscience, Yale School of Medicine, 295 Congress Ave, New Haven, CT 06510
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Caroline Kuzoian
1Department of Neuroscience, Yale School of Medicine, 295 Congress Ave, New Haven, CT 06510
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kristen J Verhey
2Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shaul Yogev
1Department of Neuroscience, Yale School of Medicine, 295 Congress Ave, New Haven, CT 06510
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: shaul.yogev@yale.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Precise synaptic connectivity defines neuronal circuits. Synapse formation is locally determined by transmembrane proteins, yet synaptic material is synthesized remotely and undergoes processive transport in axons. How local synaptogenic signals intercept synaptic cargo in transport to promote its delivery and synapse formation is unknown. We found that control of cargo delivery from microtubule minus-ends mediates pro- and anti-synaptogenic activities of presynaptic Neurexin and Frizzled in C. elegans, and identified the atypical kinesin VAB-8/KIF26 as a key molecule in this process. VAB-8/KIF26 levels on synaptic microtubule minus-ends are controlled by Frizzled and Neurexin, its loss mimics neurexin mutants or Frizzled hyperactivation, and its overexpression can rescue synapse-loss in these backgrounds. VAB-8/KIF26 protects other minus-end proteins and promotes pausing of retrograde transport to allow delivery into synapses. Consistently, reducing retrograde transport rescues synapse-loss in vab-8 and neurexin mutants. These results uncover an important mechanistic link between synaptogenic signaling and axonal transport.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted March 22, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Neurexin and Frizzled signaling intercept axonal-transport at microtubule minus-ends to control synapse formation
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Neurexin and Frizzled signaling intercept axonal-transport at microtubule minus-ends to control synapse formation
Santiago Balseiro-Gómez, Yang Yue, Lin Shao, Selim Ҫetinkaya, Caroline Kuzoian, Kristen J Verhey, Shaul Yogev
bioRxiv 2021.03.22.436477; doi: https://doi.org/10.1101/2021.03.22.436477
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Neurexin and Frizzled signaling intercept axonal-transport at microtubule minus-ends to control synapse formation
Santiago Balseiro-Gómez, Yang Yue, Lin Shao, Selim Ҫetinkaya, Caroline Kuzoian, Kristen J Verhey, Shaul Yogev
bioRxiv 2021.03.22.436477; doi: https://doi.org/10.1101/2021.03.22.436477

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (2646)
  • Biochemistry (5259)
  • Bioengineering (3671)
  • Bioinformatics (15788)
  • Biophysics (7247)
  • Cancer Biology (5624)
  • Cell Biology (8088)
  • Clinical Trials (138)
  • Developmental Biology (4763)
  • Ecology (7510)
  • Epidemiology (2059)
  • Evolutionary Biology (10571)
  • Genetics (7727)
  • Genomics (10125)
  • Immunology (5187)
  • Microbiology (13896)
  • Molecular Biology (5383)
  • Neuroscience (30754)
  • Paleontology (215)
  • Pathology (876)
  • Pharmacology and Toxicology (1524)
  • Physiology (2253)
  • Plant Biology (5017)
  • Scientific Communication and Education (1040)
  • Synthetic Biology (1384)
  • Systems Biology (4145)
  • Zoology (811)