Abstract
The utilization of RNA interference (RNAi) for pest management has garnered global interest. The bioassay results suggested the knockout of PxRdl2 significantly increased the insecticidal activities of the γ-aminobutyric acid receptor (GABAR) targeting compounds (Fipronil, two pyrazoloquinazolines and two isoxazolines), thereby presenting a viable target gene for RNAi-mediated pest control. Consequently, we suggest enhancing the insecticidal activities of GABAR-targeting compounds by knockdown the transcript level of PxRdl2. Furthermore, PxRdl2 dsRNA was expressed in HT115 Escherichia coli to reduce costs and protect dsRNA against degradation. In comparison to in vitro synthesized dsRNA, the recombinant bacteria (ds-B) exhibited superior interference efficiencies and greater stability when exposed to UV irradiation. Collectively, our results provide a new strategy of insecticide spray which combined synergistically with insecticidal activities by suppressing PxRdl2 using ds-B, and may be beneficial for reducing the usage of insecticide and slowing pest resistance.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
We have revised the introduction and discussion, and also added the results of Electrophysiological recording. Benjie Li and Kunkun Wang contributed equally to the work.
