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The von Willebrand Factor stamps Plasmatic Extracellular Vesicles from Glioblastoma Patients

Quentin Sabbagh, Gwennan André-Grégoire, Carolina Alves-Nicolau, View ORCID ProfileNicolas Bidère, Emmanuel Jouglar, Laëtitia Guével, Jean-Sébastien Frénel, Julie Gavard
doi: https://doi.org/10.1101/2021.03.25.437073
Quentin Sabbagh
1CRCINA, Inserm, CNRS, Universite de Nantes, Nantes, France
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Gwennan André-Grégoire
1CRCINA, Inserm, CNRS, Universite de Nantes, Nantes, France
2Integrated Center for Oncology, Institut de Cancerologie de l’Ouest, St. Herblain, France
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Carolina Alves-Nicolau
1CRCINA, Inserm, CNRS, Universite de Nantes, Nantes, France
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Nicolas Bidère
1CRCINA, Inserm, CNRS, Universite de Nantes, Nantes, France
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  • ORCID record for Nicolas Bidère
Emmanuel Jouglar
2Integrated Center for Oncology, Institut de Cancerologie de l’Ouest, St. Herblain, France
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Laëtitia Guével
1CRCINA, Inserm, CNRS, Universite de Nantes, Nantes, France
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Jean-Sébastien Frénel
1CRCINA, Inserm, CNRS, Universite de Nantes, Nantes, France
2Integrated Center for Oncology, Institut de Cancerologie de l’Ouest, St. Herblain, France
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Julie Gavard
1CRCINA, Inserm, CNRS, Universite de Nantes, Nantes, France
2Integrated Center for Oncology, Institut de Cancerologie de l’Ouest, St. Herblain, France
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  • For correspondence: julie.gavard@inserm.fr
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Abstract

Glioblastoma is a devastating tumor of the central nervous system characterized by a poor survival and an extremely dark prognosis, making its diagnosis, treatment and monitoring highly challenging. Numerous studies have highlighted extracellular vesicles (EVs) as key players of tumor growth, invasiveness and resistance, as they carry and disseminate oncogenic material in the local tumor microenvironment and at distance. However, whether their quality and quantity reflect individual health status and changes in homeostasis is still not fully elucidated. Here, we separated EVs from plasma collected at different time points alongside with the clinical management of GBM patients. Our findings confirm that plasmatic EVs could be separated and characterized with standardized protocols, thereby ensuring the reliability of measuring vesiclemia, i.e. extracellular vesicle concentration in plasma. This unveils that vesiclemia is a dynamic parameter, which could be reflecting tumor burden and/or response to treatments. Further label-free liquid chromatography tandem mass spectrometry unmasks the von Willebrand Factor (VWF) as a selective protein hallmark for GBM-patient EVs. Our data thus support the notion that EVs from GBM patients showed differential protein cargos that can be further surveyed in circulating EVs, together with vesiclemia.

Competing Interest Statement

The authors have declared no competing interest.

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Posted March 25, 2021.
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The von Willebrand Factor stamps Plasmatic Extracellular Vesicles from Glioblastoma Patients
Quentin Sabbagh, Gwennan André-Grégoire, Carolina Alves-Nicolau, Nicolas Bidère, Emmanuel Jouglar, Laëtitia Guével, Jean-Sébastien Frénel, Julie Gavard
bioRxiv 2021.03.25.437073; doi: https://doi.org/10.1101/2021.03.25.437073
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The von Willebrand Factor stamps Plasmatic Extracellular Vesicles from Glioblastoma Patients
Quentin Sabbagh, Gwennan André-Grégoire, Carolina Alves-Nicolau, Nicolas Bidère, Emmanuel Jouglar, Laëtitia Guével, Jean-Sébastien Frénel, Julie Gavard
bioRxiv 2021.03.25.437073; doi: https://doi.org/10.1101/2021.03.25.437073

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