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An 18S V4 rDNA metabarcoding dataset of protist diversity in the Atlantic inflow to the Arctic Ocean, through the year and down to 1000 m depth

View ORCID ProfileElianne Egge, View ORCID ProfileStephanie Elferink, View ORCID ProfileDaniel Vaulot, View ORCID ProfileUwe John, View ORCID ProfileGunnar Bratbak, View ORCID ProfileAud Larsen, View ORCID ProfileBente Edvardsen
doi: https://doi.org/10.1101/2021.03.29.437475
Elianne Egge
1University of Oslo, Department of Biosciences, Section for Aquatic Biology and Toxicology, PO Box 1066 Blindern, NO-0316 Oslo, NORWAY
2University of Duisburg-Essen, Fakultät für Biologie, Universitätsstr. 5, DE-45141 Essen, GERMANY
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  • For correspondence: elianne.egge@gmail.com
Stephanie Elferink
3Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung. Am Handelshafen 12, Bremerhaven DE-27570, GERMANY
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Daniel Vaulot
4UMR7144, CNRS, Sorbonne Université, Station Biologique de Roscoff. Place Georges Teissier, FR-29682 Roscoff, FRANCE
5Asian School of the Environment, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798 SINGAPORE
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Uwe John
3Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung. Am Handelshafen 12, Bremerhaven DE-27570, GERMANY
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Gunnar Bratbak
6University of Bergen, Department of Biological Sciences, PO Box 7803, NO-5020 Bergen, NORWAY
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Aud Larsen
7NORCE Norwegian Research Centre, PO Box 7810, NO-5020 Bergen, NORWAY
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Bente Edvardsen
1University of Oslo, Department of Biosciences, Section for Aquatic Biology and Toxicology, PO Box 1066 Blindern, NO-0316 Oslo, NORWAY
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Abstract

Arctic marine protist communities have been understudied due to challenging sampling conditions, in particular during winter and in deep waters. The aim of this study was to improve our knowledge on Arctic protist diversity through the year, both in the epipelagic (< 200 m depth) and mesopelagic zones (200-1000 m depth). Sampling campaigns were performed in 2014, during five different months, to capture the various phases of the Arctic primary production: January (winter), March (pre-bloom), May (spring bloom), August (post-bloom) and November (early winter). The cruises were undertaken west and north of the Svalbard archipelago, where warmer Atlantic waters from the West Spitsbergen Current meets cold Arctic waters from the Arctic Ocean. From each cruise, station, and depth, 50 L of sea water were collected and the plankton was size-fractionated by serial filtration into four size fractions between 0.45-200 µm, representing the picoplankton, nanoplankton and microplankton. In addition vertical net hauls were taken from 50 m depth to the surface at selected stations. From the plankton samples DNA was extracted, the V4 region of the 18S rRNA-gene was amplified by PCR with universal eukaryote primers and the amplicons were sequenced by Illumina high-throughput sequencing. Sequences were clustered into Amplicon Sequence Variants (ASVs), representing protist genotypes, with the dada2 pipeline. Taxonomic classification was made against the curated Protist Ribosomal Reference database (PR2). Altogether 6,536 protist ASVs were obtained (including 54 fungal ASVs). Both ASV richness and taxonomic composition were strongly dependent on size-fraction, season, and depth. ASV richness was generally higher in the smaller fractions, and higher in winter and the mesopelagic samples than in samples from the well-lit epipelagic zone during summer. During spring and summer, the phytoplankton groups diatoms, chlorophytes and haptophytes dominated in the epipelagic zone. Parasitic and heterotrophic groups such as Syndiniales and certain dinoflagel-lates dominated in the mesopelagic zone all year, as well as in the epipelagic zone during the winter. The dataset is available at https://doi.org/10.17882/79823, (Egge et al., 2014).

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://doi.org/10.17882/79823

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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An 18S V4 rDNA metabarcoding dataset of protist diversity in the Atlantic inflow to the Arctic Ocean, through the year and down to 1000 m depth
Elianne Egge, Stephanie Elferink, Daniel Vaulot, Uwe John, Gunnar Bratbak, Aud Larsen, Bente Edvardsen
bioRxiv 2021.03.29.437475; doi: https://doi.org/10.1101/2021.03.29.437475
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An 18S V4 rDNA metabarcoding dataset of protist diversity in the Atlantic inflow to the Arctic Ocean, through the year and down to 1000 m depth
Elianne Egge, Stephanie Elferink, Daniel Vaulot, Uwe John, Gunnar Bratbak, Aud Larsen, Bente Edvardsen
bioRxiv 2021.03.29.437475; doi: https://doi.org/10.1101/2021.03.29.437475

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