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An automated feeding system for the African killifish reveals effects of dietary restriction on lifespan and allows scalable assessment of associative learning

Andrew McKay, Chi-Kuo Hu, Sharon Chen, Claire Nicole Bedbrook, Mike Thielvoldt, Tony Wyss-Coray, Anne Brunet
doi: https://doi.org/10.1101/2021.03.30.437790
Andrew McKay
1Department of Genetics, Stanford University, Stanford, CA94305
2Biology Graduate Program, Stanford University, Stanford, CA94305
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Chi-Kuo Hu
1Department of Genetics, Stanford University, Stanford, CA94305
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Sharon Chen
1Department of Genetics, Stanford University, Stanford, CA94305
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Claire Nicole Bedbrook
1Department of Genetics, Stanford University, Stanford, CA94305
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Mike Thielvoldt
4Thielvoldt Engineering, Albany, CA94706
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Tony Wyss-Coray
3Department of Neurology and Neurological Sciences, Stanford University, Stanford, CA94305
5Glenn Laboratories for the Biology of Aging, Stanford University, CA94305
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Anne Brunet
1Department of Genetics, Stanford University, Stanford, CA94305
5Glenn Laboratories for the Biology of Aging, Stanford University, CA94305
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  • For correspondence: abrunet1@stanford.edu
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Abstract

The African turquoise killifish is an exciting new vertebrate model for aging studies. A significant challenge for any model organism is control over its diet in space and time. To address this challenge, we created an automated and networked fish feeding system. Our automated feeder is designed to be open-source, easily transferable, and built from widely available components. Compared to manual feeding, our automated system is highly precise and flexible. As a proof-of-concept for the feeding schedule flexibility of these automated feeders, we define a favorable regimen for growth and fertility for the African killifish and a dietary restriction regimen where both feeding time and quantity are reduced. We show that this dietary restriction regimen extends lifespan in males. Moreover, combining our automated feeding system with a video camera, we establish an associative learning assay for the killifish. This learning assay provides an integrative measure of cognitive decline during aging. The ability to precisely control food delivery in the killifish opens new areas to assess lifespan and cognitive behavior dynamics and to screen for dietary interventions and drugs in a high-throughput manner previously impossible with traditional vertebrate model organisms.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted March 31, 2021.
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An automated feeding system for the African killifish reveals effects of dietary restriction on lifespan and allows scalable assessment of associative learning
Andrew McKay, Chi-Kuo Hu, Sharon Chen, Claire Nicole Bedbrook, Mike Thielvoldt, Tony Wyss-Coray, Anne Brunet
bioRxiv 2021.03.30.437790; doi: https://doi.org/10.1101/2021.03.30.437790
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An automated feeding system for the African killifish reveals effects of dietary restriction on lifespan and allows scalable assessment of associative learning
Andrew McKay, Chi-Kuo Hu, Sharon Chen, Claire Nicole Bedbrook, Mike Thielvoldt, Tony Wyss-Coray, Anne Brunet
bioRxiv 2021.03.30.437790; doi: https://doi.org/10.1101/2021.03.30.437790

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