Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Endosomal recycling to the surface mediated by Gpa1 and PI3-Kinase is inhibited by glucose starvation

View ORCID ProfileKamilla ME. Laidlaw, View ORCID ProfileKatherine M. Paine, View ORCID ProfileDaniel D. Bisinski, Grant Calder, View ORCID ProfileChris MacDonald
doi: https://doi.org/10.1101/2021.04.02.438183
Kamilla ME. Laidlaw
1York Biomedical Research Institute and Department of Biology, University of York, York, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kamilla ME. Laidlaw
Katherine M. Paine
1York Biomedical Research Institute and Department of Biology, University of York, York, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Katherine M. Paine
Daniel D. Bisinski
1York Biomedical Research Institute and Department of Biology, University of York, York, UK
2Department of Biology & Chemistry, University of Osnabrück, Osnabrück, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Daniel D. Bisinski
Grant Calder
3Imaging and Cytometry Laboratory, Bioscience Technology Facility, Department of Biology, University of York, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chris MacDonald
1York Biomedical Research Institute and Department of Biology, University of York, York, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Chris MacDonald
  • For correspondence: chris.macdonald@york.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

Cell surface protein trafficking is regulated in response to nutrient availability, with multiple pathways directing surface membrane proteins to the lysosome for degradation in response to suboptimal extracellular nutrients. Internalised protein and lipid cargoes recycle back to the surface efficiently in glucose replete conditions, but this trafficking is attenuated following glucose starvation. We find cells with either reduced or hyperactive phosphatidylinositol 3-kinase activity are defective for recycling. Furthermore, we find the yeast Gα subunit Gpa1, which localises to endosomes and functionally couples with phosphatidylinositol 3-kinase, is required for surface recycling of cargoes. Following glucose starvation, nuclear translocation of Mig1 increases GPA2 levels and inhibits recycling, potentially by diverting Gpa1 to the surface and interfering with its endosomal role in recycling. Glucose privation therefore triggers a survival mechanism to increase retention of surface cargoes in endosomes and promote their lysosomal degradation.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted April 02, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Endosomal recycling to the surface mediated by Gpa1 and PI3-Kinase is inhibited by glucose starvation
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Endosomal recycling to the surface mediated by Gpa1 and PI3-Kinase is inhibited by glucose starvation
Kamilla ME. Laidlaw, Katherine M. Paine, Daniel D. Bisinski, Grant Calder, Chris MacDonald
bioRxiv 2021.04.02.438183; doi: https://doi.org/10.1101/2021.04.02.438183
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Endosomal recycling to the surface mediated by Gpa1 and PI3-Kinase is inhibited by glucose starvation
Kamilla ME. Laidlaw, Katherine M. Paine, Daniel D. Bisinski, Grant Calder, Chris MacDonald
bioRxiv 2021.04.02.438183; doi: https://doi.org/10.1101/2021.04.02.438183

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3605)
  • Biochemistry (7575)
  • Bioengineering (5529)
  • Bioinformatics (20806)
  • Biophysics (10333)
  • Cancer Biology (7986)
  • Cell Biology (11644)
  • Clinical Trials (138)
  • Developmental Biology (6610)
  • Ecology (10214)
  • Epidemiology (2065)
  • Evolutionary Biology (13622)
  • Genetics (9543)
  • Genomics (12851)
  • Immunology (7923)
  • Microbiology (19550)
  • Molecular Biology (7666)
  • Neuroscience (42125)
  • Paleontology (308)
  • Pathology (1258)
  • Pharmacology and Toxicology (2203)
  • Physiology (3268)
  • Plant Biology (7044)
  • Scientific Communication and Education (1294)
  • Synthetic Biology (1951)
  • Systems Biology (5427)
  • Zoology (1118)