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The hexose transporter SWEET5 confers galactose sensitivity to Arabidopsis pollen germination via the galactokinase GALK

Jiang Wang, View ORCID ProfileYa-Chi Yu, Ye Li, View ORCID ProfileLi-Qing Chen
doi: https://doi.org/10.1101/2021.04.07.438853
Jiang Wang
1Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA
2Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA
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Ya-Chi Yu
1Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA
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Ye Li
1Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA
3Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 ZhongGuanCun South Street, Beijing, 100081, China
4Current address: Key Laboratory of Agricultural Products Chemical and Biological Processing Technology, Zhejiang University of Science and Technology, Hangzhou 310023, China
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Li-Qing Chen
1Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA
2Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA
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  • For correspondence: lqchen77@illinois.edu
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Abstract

Galactose is an abundant and essential sugar used for the biosynthesis of many macromolecules in different organisms, including plants. Galactose metabolism is tightly and finely controlled since excess galactose and derivatives are inhibitory. In Arabidopsis, root growth and pollen germination were strongly inhibited by excess galactose. However, the mechanism of galactose induced inhibition during pollen germination remains obscure. In this study, we characterized a plasma-membrane localized transporter, AtSWEET5, that transports glucose and galactose. SWEET5 protein level started to accumulate at the tricellular stage of pollen development and peaked in mature pollen before rapidly declining after pollen was germinated. SWEET5 levels are responsible for the dosage-dependent sensitivity of galactose and galactokinase (GALK) is essential for the inhibitory effects of galactose during pollen germination. Overall, SWEET5 and GALK contribute to the maintenance of galactose metabolic homeostasis during pollen germination. SWEET5 serves as a major low-affinity hexose transporter at the early stage of pollen germination.

One-sentence summary SWEET5 mediates pollen galactose sensitivity via GALK that is required for efficient galactose uptake in pollen during pollen germination.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 15, 2021.
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The hexose transporter SWEET5 confers galactose sensitivity to Arabidopsis pollen germination via the galactokinase GALK
Jiang Wang, Ya-Chi Yu, Ye Li, Li-Qing Chen
bioRxiv 2021.04.07.438853; doi: https://doi.org/10.1101/2021.04.07.438853
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The hexose transporter SWEET5 confers galactose sensitivity to Arabidopsis pollen germination via the galactokinase GALK
Jiang Wang, Ya-Chi Yu, Ye Li, Li-Qing Chen
bioRxiv 2021.04.07.438853; doi: https://doi.org/10.1101/2021.04.07.438853

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