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Volumetric morphometry reveals mitotic spindle width as the best predictor of spindle scaling

Tobias Kletter, Sebastian Reusch, Nils Dempewolf, View ORCID ProfileChristian Tischer, Simone Reber
doi: https://doi.org/10.1101/2021.04.08.438956
Tobias Kletter
1IRI Life Sciences, Humboldt-Universität zu Berlin, Berlin, Germany
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Sebastian Reusch
1IRI Life Sciences, Humboldt-Universität zu Berlin, Berlin, Germany
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Nils Dempewolf
3University of Applied Sciences Berlin, Berlin, Germany
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Christian Tischer
2ALMF, EMBL, Heidelberg, Germany
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  • ORCID record for Christian Tischer
Simone Reber
1IRI Life Sciences, Humboldt-Universität zu Berlin, Berlin, Germany
3University of Applied Sciences Berlin, Berlin, Germany
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  • For correspondence: simone.reber@iri-lifesciences.de
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Abstract

The function of cellular structures at the mesoscale is dependent on their geometry and proportionality to cell size. The mitotic spindle is a good example why length and shape of intracellular organelles matter. Spindle length determines the distance over which chromosomes will segregate and spindle shape ensures bipolarity. While we still lack a systematic and quantitative understanding of subcellular morphometrics, new imaging techniques and volumetric data analysis promise novel insights into scaling relations across different species. Here, we introduce Spindle3D, an open-source plug-in that allows for the quantitative, unbiased, and automated analysis of 3D fluorescent data of spindles and chromatin. We systematically analyse different cell types, including somatic cells, stem cells and one-cell embryos across different phyla to derive volumetric relations of spindle, chromatin, and cell volume. Taken together, our data indicate that mitotic spindle width is a robust indicator of spindle volume, which correlates linearly with chromatin and cell volume both within single cell types and across metazoan phyla.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted April 08, 2021.
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Volumetric morphometry reveals mitotic spindle width as the best predictor of spindle scaling
Tobias Kletter, Sebastian Reusch, Nils Dempewolf, Christian Tischer, Simone Reber
bioRxiv 2021.04.08.438956; doi: https://doi.org/10.1101/2021.04.08.438956
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Volumetric morphometry reveals mitotic spindle width as the best predictor of spindle scaling
Tobias Kletter, Sebastian Reusch, Nils Dempewolf, Christian Tischer, Simone Reber
bioRxiv 2021.04.08.438956; doi: https://doi.org/10.1101/2021.04.08.438956

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