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Plaque Size Tool: an automated plaque analysis tool for simplifying and standardising bacteriophage plaque morphology measurements

View ORCID ProfileEllina Trofimova, View ORCID ProfilePaul R Jaschke
doi: https://doi.org/10.1101/2021.04.12.439404
Ellina Trofimova
1Department of Molecular Sciences, Macquarie University, Balaclava Rd, Macquarie Park NSW 2109, Australia
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Paul R Jaschke
1Department of Molecular Sciences, Macquarie University, Balaclava Rd, Macquarie Park NSW 2109, Australia
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  • For correspondence: paul.jaschke@mq.edu.au
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Abstract

Bacteriophage plaque size measurement is essential for phage characterisation, but their manual size estimation requires a considerable amount of time and effort. In order to ease the work of phage researchers, we have developed an automated command-line application called Plaque Size Tool (PST) that can detect plaques of different morphology on the images of Petri dishes and measure plaque area and diameter. Plaque size measurements using PST showed no difference to those obtained with manual plaque size measurement in Fiji, indicating future results using PST are backwards compatible with prior measurements in the literature. PST can be applied to a range of lytic bacteriophages producing oval-shaped plaques, including bull’s-eye morphology. The application can also be used for titer calculation if most of the plaques are stand-alone. As laboratory automation becomes more commonplace, standardised and flexible open-source analytical tools like PST will be important parts of biofoundry and cloud lab bacteriophage workflows.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://github.com/ellinium/plaque_size_tool

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 12, 2021.
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Plaque Size Tool: an automated plaque analysis tool for simplifying and standardising bacteriophage plaque morphology measurements
Ellina Trofimova, Paul R Jaschke
bioRxiv 2021.04.12.439404; doi: https://doi.org/10.1101/2021.04.12.439404
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Plaque Size Tool: an automated plaque analysis tool for simplifying and standardising bacteriophage plaque morphology measurements
Ellina Trofimova, Paul R Jaschke
bioRxiv 2021.04.12.439404; doi: https://doi.org/10.1101/2021.04.12.439404

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