Abstract
Background Efficient interhemispheric integration of neural activity between left and right primary motor cortex (M1) is critical for inter-limb motor control.
Objective We employed optogenetic stimulation to establish a framework for probing transcallosal M1-M1 interactions in rats.
Methods In male rats, we optogenetically stimulated glutamatergic neurons in right M1 and recorded the transcallosally evoked potential with chronically implanted electrodes in contralateral left M1 during dexmedetomidine anesthesia. We systematically varied the stimulation intensity and duration to characterize the relationship between stimulation parameters in right M1 and the characteristics of the evoked intracortical potentials in left M1.
Results Optogenetic stimulation of right M1 consistently evoked a transcallosal response in left M1 with a consistent negative peak (N1) that sometimes was preceded by a smaller positive peak (P1). Higher stimulation intensity or longer stimulation duration gradually increased N1 amplitude and reduced N1 variability across trials. Median N1 latencies remained stable, once stimulation elicited a reliable N1 peak and did not display a systematic shortening with increasing stimulation intensity or duration.
Conclusions Optogenetically stimulated glutamatergic neurons in M1 can reliably evoke a transcallosal response in anesthetized rats and can be used to characterize the relationship between “stimulation dose” and “response magnitude” (i.e., the gain function) of transcallosal M1-to-M1 glutamatergic connections. Detailed knowledge of the stimulus-response relationship is needed to optimize the efficacy of optogenetic stimulation. Since transcallosal M1-M1 interactions can also be probed non-invasively with transcranial magnetic stimulation in humans, our optogenetic stimulation approach bears translational potential for studying how unilateral M1 stimulation can induce interhemispheric plasticity.
Competing Interest Statement
CSS was kindly provided with dental acrylic cement, mixing apparatus and application tools by GC Europe. HRS has received honoraria as speaker from Sanofi Genzyme, Denmark and Novartis, Denmark, as consultant from Sanofi Genzyme, Denmark, Lophora A/S, Denmark, Lundbeck Pharma A/S, Denmark, and as editor in chief (Neuroimage Clinical) and senior editor (NeuroImage) from Elsevier Publishers, Amsterdam, The Netherlands. He has received royalties as book editor from Springer Publishers, Stuttgart, Germany and Gyldendal Publishers, Copenhagen, Denmark. Funding HRS holds a 5-year professorship in precision medicine at the Faculty of Health Sciences and Medicine, University of Copenhagen which is sponsored by the Lundbeck Foundation (grant number R186-2015-2138). CSS was supported by Capital Region Research Foundation Grant (grant number A5657; principal investigator: TBD). The travel in relation to the presentation of part of the data at the Society for Neuroscience - Neuroscience Meeting 2019, was financially supported by Lundbeck Foundation travel stipend (R315-2019-915).
Footnotes
↵* Shared last authorship
Prior presentation: Part of the data was presented at a poster session by CSS at the Society for Neuroscience (SfN) - Neuroscience Meeting 2019 (Chicago, Illinois, USA). The abstract was submitted with the following title: “Transcallosal functional connectivity between motor cortices in rats probed with unilateral optogenetic stimulation of glutamatergic neurons” by the same authors.
