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4 SPECIES OF BACTERIA DETERMINISTICALLY FORM A STABLE BIOFILM IN A MILLIFLUIDIC CHANNEL: ASSEMBLY PRINCIPLES

A. Monmeyran, W. Benyoussef, P. Thomen, N. Dahmane, A. Baliarda, M. Jules, S. Aymerich, N. Henry
doi: https://doi.org/10.1101/2021.04.16.440159
A. Monmeyran
1Sorbonne Université, CNRS, Laboratoire Jean Perrin (UMR 8237), 4 place Jussieu, F-75005 Paris, France
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W. Benyoussef
1Sorbonne Université, CNRS, Laboratoire Jean Perrin (UMR 8237), 4 place Jussieu, F-75005 Paris, France
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P. Thomen
1Sorbonne Université, CNRS, Laboratoire Jean Perrin (UMR 8237), 4 place Jussieu, F-75005 Paris, France
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N. Dahmane
2Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France
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A. Baliarda
2Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France
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M. Jules
2Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France
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S. Aymerich
2Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France
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N. Henry
1Sorbonne Université, CNRS, Laboratoire Jean Perrin (UMR 8237), 4 place Jussieu, F-75005 Paris, France
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  • For correspondence: nelly.henry@upmc.fr
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Abstract

Multispecies microbial adherent communities are widespread in nature and organisms but the principles of their assembly and development remain unclear. Yet, the demand to understand and predict the responses of such living communities to environmental changes is increasing, calling for new approaches. Here, we test the possibility to establish a simplified but relevant model of multispecies biofilm in a laboratory setup enabling in situ real-time monitoring of the community development and control of the environmental parameters in order to decipher the mechanisms underlying the formation of the community. Using video-microscopy and species combinatorial approach, we assess the global and individual species spatiotemporal development in millifluidic channels under constant flow of nutrients. Based on quantitative measurements of expansion kinetics, local dynamics and spatial distribution, we demonstrate that the four chosen species (Bacillus thuringiensis, Pseudomonas fluorescens, Kocuria varians and Rhodocyclus sp.) form a dynamical community that deterministically reaches its equilibrium after about 30 hours of growth. We evidence the emergence of complexity in this simplified community as reported by spatial heterogeneity rise and non-monotonic developmental kinetics. We find interspecies interactions consisting in competition for resources — in particular oxygen — and both direct and indirect physical interactions but no positive feedback. Thereby, we introduce a model of multispecies adherent community where effective couplings result from individual species quest for fitness optimization in a moving and heterogenous environment. This control and the understanding of this simplified experimental model shall open new avenues to apprehend adherent bacterial communities behavior in a context of rapid global change.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Competing InterestsThe authors declare there are no competing interests

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted April 16, 2021.
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4 SPECIES OF BACTERIA DETERMINISTICALLY FORM A STABLE BIOFILM IN A MILLIFLUIDIC CHANNEL: ASSEMBLY PRINCIPLES
A. Monmeyran, W. Benyoussef, P. Thomen, N. Dahmane, A. Baliarda, M. Jules, S. Aymerich, N. Henry
bioRxiv 2021.04.16.440159; doi: https://doi.org/10.1101/2021.04.16.440159
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4 SPECIES OF BACTERIA DETERMINISTICALLY FORM A STABLE BIOFILM IN A MILLIFLUIDIC CHANNEL: ASSEMBLY PRINCIPLES
A. Monmeyran, W. Benyoussef, P. Thomen, N. Dahmane, A. Baliarda, M. Jules, S. Aymerich, N. Henry
bioRxiv 2021.04.16.440159; doi: https://doi.org/10.1101/2021.04.16.440159

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