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Flexible Simultaneous Mesoscale Two-Photon Imaging Of Neural Activity at High Speeds

Mitchell Clough, Ichun Anderson Chen, Seong-Wook Park, Allison M. Ahrens, Jeffrey N. Stirman, Spencer L. Smith, Jerry L. Chen
doi: https://doi.org/10.1101/2021.04.19.440507
Mitchell Clough
1Department of Biomedical Engineering, Boston University, Boston MA 02215, USA
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Ichun Anderson Chen
1Department of Biomedical Engineering, Boston University, Boston MA 02215, USA
2Center for Neurophotonics, Boston University, Boston MA 02215, USA
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Seong-Wook Park
1Department of Biomedical Engineering, Boston University, Boston MA 02215, USA
2Center for Neurophotonics, Boston University, Boston MA 02215, USA
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Allison M. Ahrens
3Department of Biology, Boston University, Boston MA 02215, USA
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Jeffrey N. Stirman
4Neuroscience Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
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Spencer L. Smith
5Department of Electrical and Computer Engineering, University of California Santa Barbara, Santa Barbara CA 93106, USA
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Jerry L. Chen
1Department of Biomedical Engineering, Boston University, Boston MA 02215, USA
2Center for Neurophotonics, Boston University, Boston MA 02215, USA
3Department of Biology, Boston University, Boston MA 02215, USA
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  • For correspondence: jerry@chen-lab.org
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ABSTRACT

Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 20, 2021.
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Flexible Simultaneous Mesoscale Two-Photon Imaging Of Neural Activity at High Speeds
Mitchell Clough, Ichun Anderson Chen, Seong-Wook Park, Allison M. Ahrens, Jeffrey N. Stirman, Spencer L. Smith, Jerry L. Chen
bioRxiv 2021.04.19.440507; doi: https://doi.org/10.1101/2021.04.19.440507
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Flexible Simultaneous Mesoscale Two-Photon Imaging Of Neural Activity at High Speeds
Mitchell Clough, Ichun Anderson Chen, Seong-Wook Park, Allison M. Ahrens, Jeffrey N. Stirman, Spencer L. Smith, Jerry L. Chen
bioRxiv 2021.04.19.440507; doi: https://doi.org/10.1101/2021.04.19.440507

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