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Quorum sensing signals from epibiont mediate the induction of bioactive peptides in mat-forming cyanobacteria Nostoc

Subhasish Saha, Paul-Adrian Bulzu, Petra Urajová, View ORCID ProfileJan Mareš, Grzegorz Konert, João Câmara Manoel, Markéta Macho, Daniela Ewe, View ORCID ProfilePavel Hrouzek, Jiří Masojídek, View ORCID ProfileRohit Ghai, View ORCID ProfileKumar Saurav
doi: https://doi.org/10.1101/2021.04.23.441229
Subhasish Saha
aLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center Algatech, Trebon, Czech Republic
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Paul-Adrian Bulzu
bBiology Centre of the Czech Academy of Sciences, Institute of Hydrobiology, České Budějovice, Czech Republic
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Petra Urajová
aLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center Algatech, Trebon, Czech Republic
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Jan Mareš
aLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center Algatech, Trebon, Czech Republic
bBiology Centre of the Czech Academy of Sciences, Institute of Hydrobiology, České Budějovice, Czech Republic
cUniversity of South Bohemia, Faculty of Science, České Budějovice, Czech Republic
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Grzegorz Konert
aLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center Algatech, Trebon, Czech Republic
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João Câmara Manoel
aLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center Algatech, Trebon, Czech Republic
cUniversity of South Bohemia, Faculty of Science, České Budějovice, Czech Republic
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Markéta Macho
aLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center Algatech, Trebon, Czech Republic
cUniversity of South Bohemia, Faculty of Science, České Budějovice, Czech Republic
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Daniela Ewe
aLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center Algatech, Trebon, Czech Republic
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Pavel Hrouzek
aLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center Algatech, Trebon, Czech Republic
cUniversity of South Bohemia, Faculty of Science, České Budějovice, Czech Republic
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Jiří Masojídek
aLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center Algatech, Trebon, Czech Republic
cUniversity of South Bohemia, Faculty of Science, České Budějovice, Czech Republic
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Rohit Ghai
bBiology Centre of the Czech Academy of Sciences, Institute of Hydrobiology, České Budějovice, Czech Republic
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Kumar Saurav
aLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center Algatech, Trebon, Czech Republic
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  • For correspondence: sauravverma17@gmail.com
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ABSTRACT

The regulation of oligopeptides production is essential in understanding their ecological role in complex microbial communities including harmful cyanobacterial blooms. The role of chemical communication between the cyanobacterium and the microbial community harboured as epibionts within its phycosphere is at an initial stage of research and little is understood about its specificity. Herein, we present insight into the role of a bacterial epibiont in regulating production of cyanobacterial oligopeptides microviridins, well-known elastase inhibitors with presumed anti-grazing effects, in an ecologically important cyanobacterial genus Nostoc. Heterologous expression and identification of specific signal molecules from the epibiont suggest the role of a quorum sensing-based interaction. Further, physiological experiments show an increase in microviridin production without affecting cyanobacterial growth and photosynthetic activity. Simultaneously, oligopeptides presenting a selective inhibition pattern provide support for their specific function in response to the presence of cohabitant epibionts. Thus, the chemical interaction revealed in our study provides an example of an interspecies signalling pathway monitoring the bacterial flora around the cyanobacterial filaments and induction of intrinsic species-specific metabolic responses.

IMPORTANCE The regulation of cyanopeptide production beyond microcystin is essential to understand their ecological role in complex microbial communities, e.g. harmful cyanobacterial blooms. The role of chemical communication between the cyanobacterium and the epibionts within its phycosphere is at an initial stage of research and little is understood about its specificity. The frequency of cyanopeptide occurrence also demonstrates the need to understand the contribution of cyanobacterial peptides to the overall biological impact of cyanopeptides on aquatic organisms and vertebrates including humans. Our results shed light on the epibiont control of cyanopeptide production via quorum sensing mechanisms and we posit that such mechanisms may be widespread in natural cyanobacterial bloom community regulation.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 24, 2021.
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Quorum sensing signals from epibiont mediate the induction of bioactive peptides in mat-forming cyanobacteria Nostoc
Subhasish Saha, Paul-Adrian Bulzu, Petra Urajová, Jan Mareš, Grzegorz Konert, João Câmara Manoel, Markéta Macho, Daniela Ewe, Pavel Hrouzek, Jiří Masojídek, Rohit Ghai, Kumar Saurav
bioRxiv 2021.04.23.441229; doi: https://doi.org/10.1101/2021.04.23.441229
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Quorum sensing signals from epibiont mediate the induction of bioactive peptides in mat-forming cyanobacteria Nostoc
Subhasish Saha, Paul-Adrian Bulzu, Petra Urajová, Jan Mareš, Grzegorz Konert, João Câmara Manoel, Markéta Macho, Daniela Ewe, Pavel Hrouzek, Jiří Masojídek, Rohit Ghai, Kumar Saurav
bioRxiv 2021.04.23.441229; doi: https://doi.org/10.1101/2021.04.23.441229

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