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Dual reference method for high precision infrared measurement of leaf surface temperature under field conditions

View ORCID ProfileJonathan D. Muller, View ORCID ProfileEyal Rotenberg, View ORCID ProfileFyodor Tatarinov, Irina Vishnevetsky, View ORCID ProfileTamir Dingjan, View ORCID ProfileAbraham Kribus, View ORCID ProfileDan Yakir
doi: https://doi.org/10.1101/2021.04.25.440729
Jonathan D. Muller
1Department of Earth and Planetary Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel
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  • ORCID record for Jonathan D. Muller
Eyal Rotenberg
1Department of Earth and Planetary Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel
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  • For correspondence: eyal.rotenberg@weizmann.ac.il
Fyodor Tatarinov
1Department of Earth and Planetary Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel
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Irina Vishnevetsky
1Department of Earth and Planetary Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel
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Tamir Dingjan
2Department of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel
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Abraham Kribus
3School of Mechanical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv 69978, Israel
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Dan Yakir
1Department of Earth and Planetary Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel
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  • ORCID record for Dan Yakir
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Abstract

  • Temperature is a key control over biological activities from the cellular to the ecosystem scales. However, direct, high precision measurements of surface temperature of small objects such as leaves under field conditions with large variations in ambient conditions remain rare. Contact methods such as thermocouples are prone to large errors. The use of non-contact remote sensing methods such as thermal infrared measurements provides an ideal solution, but their accuracy has been low (in the order of ~2 °C) due to necessity for corrections for material emissivity and fluctuations in background radiation (Lbg).

  • A novel ‘dual-reference’ method was developed to increase the accuracy of infrared needle-leaf surface temperature measurements in the field. It accounts for variations in Lbg and corrects for the systematic camera offset using two reference plates.

  • We accurately captured surface temperature and leaf-to-air temperature differences of needle-leaves in a forest ecosystem with large diurnal and seasonal temperature fluctuations with an uncertainty of ±0.23 and ±0.25 °C, respectively.

  • Routine high precision leaf temperature measurements even under harsh field conditions, such as demonstrated here, opens the way for investigating a wide range of leaf-scale processes and its dynamics.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://doi.org/10.5281/zenodo.4088156

  • https://doi.org/10.5281/zenodo.4104314

  • https://doi.org/10.5281/zenodo.4284621

  • Nomenclature

    σ
    Stefan-Boltzman constant
    τ
    Transmissivity of the air column
    ε
    Emissivity
    a, b
    slope, intercept
    L
    Thermal radiant flux
    air
    Air column between object and IR camera
    ap
    Apparent, infrared, temperature
    bg
    Thermal background radiant flux
    camera
    Camera-received radiation
    cor
    Corrected, i.e. after calibration
    emiss
    Emissive plate (∊emiss ≈ 1)
    ir
    Infrared
    obj
    Measured object of interest
    refl
    Reflective plate (∊refl ≈ 0)
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    Posted April 26, 2021.
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    Dual reference method for high precision infrared measurement of leaf surface temperature under field conditions
    Jonathan D. Muller, Eyal Rotenberg, Fyodor Tatarinov, Irina Vishnevetsky, Tamir Dingjan, Abraham Kribus, Dan Yakir
    bioRxiv 2021.04.25.440729; doi: https://doi.org/10.1101/2021.04.25.440729
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    Dual reference method for high precision infrared measurement of leaf surface temperature under field conditions
    Jonathan D. Muller, Eyal Rotenberg, Fyodor Tatarinov, Irina Vishnevetsky, Tamir Dingjan, Abraham Kribus, Dan Yakir
    bioRxiv 2021.04.25.440729; doi: https://doi.org/10.1101/2021.04.25.440729

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