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Single cell response landscape of graded Nodal signaling in zebrafish explants

Tao Cheng, Yan-Yi Xing, Yun-Fei Li, Cong Liu, Ying Huang, Ying-Jie Zhang, Sean G. Megason, View ORCID ProfilePeng-Fei Xu
doi: https://doi.org/10.1101/2021.04.25.441305
Tao Cheng
1Women’s Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China
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Yan-Yi Xing
1Women’s Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China
2Zhejiang Provincial Key Laboratory of Genetic & Developmental Disorders
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Yun-Fei Li
1Women’s Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China
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Cong Liu
1Women’s Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China
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Ying Huang
1Women’s Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China
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Ying-Jie Zhang
1Women’s Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China
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Sean G. Megason
3Department of Systems Biology, Harvard Medical School,200 Longwood Avenue, Boston MA 02115, USA
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  • For correspondence: megason@hms.harvard.edu pengfei_xu@zju.edu.cn
Peng-Fei Xu
1Women’s Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China
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  • ORCID record for Peng-Fei Xu
  • For correspondence: megason@hms.harvard.edu pengfei_xu@zju.edu.cn
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Abstract

Nodal, as a morphogen, plays important roles in cell fate decision, pattern formation and organizer function. But because of the complex context in vivo and technology limitations, systematic studying of genes, cell types and patterns induced by Nodal alone is still missing. Here, by using a relatively simplified model, the zebrafish blastula animal pole explant avoiding additional instructive signals and prepatterns, we constructed a single cell response landscape of graded Nodal signaling, identified 105 Nodal immediate targets and depicted their expression patterns. Our results show that Nodal signaling is sufficient to induce anterior-posterior patterned axial mesoderm and head structure. Surprisingly, the endoderm induced by Nodal alone is mainly the anterior endoderm which gives rise to the pharyngeal pouch only, but not internal organs. Among the 105 Nodal targets, we identified 14 genes carrying varying levels of axis induction capability. Overall, our work provides new insights for understanding of the Nodal function and a valuable resource for future studies of patterning and morphogenesis induced by it.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted April 26, 2021.
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Single cell response landscape of graded Nodal signaling in zebrafish explants
Tao Cheng, Yan-Yi Xing, Yun-Fei Li, Cong Liu, Ying Huang, Ying-Jie Zhang, Sean G. Megason, Peng-Fei Xu
bioRxiv 2021.04.25.441305; doi: https://doi.org/10.1101/2021.04.25.441305
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Single cell response landscape of graded Nodal signaling in zebrafish explants
Tao Cheng, Yan-Yi Xing, Yun-Fei Li, Cong Liu, Ying Huang, Ying-Jie Zhang, Sean G. Megason, Peng-Fei Xu
bioRxiv 2021.04.25.441305; doi: https://doi.org/10.1101/2021.04.25.441305

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