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A large lipoprotein mediates target specificity for T6SS-dependent killing

View ORCID ProfileLauren Speare, Madison Woo, Anne K. Dunn, Alecia N. Septer
doi: https://doi.org/10.1101/2021.04.26.440508
Lauren Speare
1Department of Marine Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
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Madison Woo
1Department of Marine Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
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Anne K. Dunn
2Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019
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Alecia N. Septer
1Department of Marine Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
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  • For correspondence: asepter@email.unc.edu
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Abstract

Interbacterial competition is prevalent in host-associated microbiota, where it can shape community structure and function, impacting host health in both positive and negative ways. However, the factors that permit bacteria to discriminate among their various neighbors for targeted elimination of competitors remain elusive. We identified a specificity factor in Vibrio species that is used to target specific competitors for elimination. Here, we describe this specificity factor, which is associated with the broadly-distributed type VI secretion system (T6SS), by studying symbiotic Vibrio fischeri, which use the T6SS to compete for colonization sites in their squid host. We demonstrate that a large lipoprotein (TasL) allows V. fischeri cells to restrict T6SS-dependent killing to certain genotypes by selectively integrating competitor cells into aggregates while excluding other cell types. TasL is also required for T6SS-dependent competition within juvenile squid, indicating the adhesion factor is active in the host. Because TasL homologs are found in other host-associated bacterial species, this newly-described specificity factor has the potential to impact microbiome structure within diverse hosts.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 26, 2021.
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A large lipoprotein mediates target specificity for T6SS-dependent killing
Lauren Speare, Madison Woo, Anne K. Dunn, Alecia N. Septer
bioRxiv 2021.04.26.440508; doi: https://doi.org/10.1101/2021.04.26.440508
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A large lipoprotein mediates target specificity for T6SS-dependent killing
Lauren Speare, Madison Woo, Anne K. Dunn, Alecia N. Septer
bioRxiv 2021.04.26.440508; doi: https://doi.org/10.1101/2021.04.26.440508

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