Abstract
The maize cultivar Dan340 is an excellent backbone inbred line of Luda Red Cob Group with several desirable characters, such as disease resistance, lodging resistance, high combining ability, wide adaptability and so on. In this study, we constructed a high-quality chromosome-level reference genome for Dan340 by combining PacBio long HiFi sequencing, Illumina short reads and chromosomal conformational capture (Hi-C) sequencing reads. The final assembly of Dan340 genome was 2,348.72 Mb, including 2,738 contigs and 2,315 scaffolds with a N50 of 41.49 Mb and 215.35 Mb, respectively. The percent of high quality Illumina reads mapped to the reference genome was up to 97.48%. The assembly of this genome will not only facilitate our understanding about intraspecific genome diversity in maize, but also provides a novel resource for maize breeding.
Key findings
The final assembly of Dan340 genome was 2,348.72 Mb, including 2,738 contigs and 2,315 scaffolds with a N50 of 41.49 Mb and 215.35 Mb, respectively.
The percent of reads mapped to the reference genome was up to 97.48%.
The results showed that 96.84% of the plant single-copy orthologues were complete. Complete single-copy and multi copy genes accounted for 87.36% and 9.48% of the genes, respectively. Taken together, these results indicated that our Dan340 genome assembly presented high quality and coverage.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
Update Figure: Figure 1 BUSCO Assessment Results (Database: embryophyta_odb10, n: 1614)
