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Functional characterisation of banana (Musa spp.) 2-oxoglutarate-dependent dioxygenases involved in flavonoid biosynthesis

View ORCID ProfileMareike Busche, Christopher Acatay, View ORCID ProfileStefan Martens, View ORCID ProfileBernd Weisshaar, View ORCID ProfileRalf Stracke
doi: https://doi.org/10.1101/2021.05.03.442406
Mareike Busche
1Genetics and Genomics of Plants, Faculty of Biology, Bielefeld University, 33615 Bielefeld, Germany
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Christopher Acatay
1Genetics and Genomics of Plants, Faculty of Biology, Bielefeld University, 33615 Bielefeld, Germany
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Stefan Martens
2Fondazione Edmund Mach, Research and Innovation Centre, San Michele All’ Adige, TN, Italy
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Bernd Weisshaar
1Genetics and Genomics of Plants, Faculty of Biology, Bielefeld University, 33615 Bielefeld, Germany
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Ralf Stracke
1Genetics and Genomics of Plants, Faculty of Biology, Bielefeld University, 33615 Bielefeld, Germany
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  • For correspondence: ralf.stracke@uni-bielefeld.de
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Abstract

Bananas (Musa) are non-grass, monocotyledonous, perennial plants that are well-known for their edible fruits. Their cultivation provides food security and employment opportunities in many countries. Banana fruits contain high levels of minerals and phytochemicals, including flavonoids, which are beneficial for human nutrition. To broaden the knowledge on flavonoid biosynthesis in this major crop plant, we aimed to identify and functionally characterise selected structural genes encoding 2-oxoglutarate-dependent dioxygenases, involved in the formation of the flavonoid aglycon.

Musa candidates genes predicted to encode flavanone 3-hydroxylase (F3H), flavonol synthase (FLS) and anthocyanidin synthase (ANS) were assayed. Enzymatic functionalities of the recombinant proteins were confirmed in vivo using bioconversion assays. Moreover, transgenic analyses in corresponding Arabidopsis thaliana mutants showed that MusaF3H, MusaFLS and MusaANS were able to complement the respective loss-of-function phenotypes, thus verifying functionality of the enzymes in planta.

Knowledge gained from this work provides a new aspect for further research towards genetic engineering of flavonoid biosynthesis in banana fruits to increase their antioxidant activity and nutritional value.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted July 16, 2021.
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Functional characterisation of banana (Musa spp.) 2-oxoglutarate-dependent dioxygenases involved in flavonoid biosynthesis
Mareike Busche, Christopher Acatay, Stefan Martens, Bernd Weisshaar, Ralf Stracke
bioRxiv 2021.05.03.442406; doi: https://doi.org/10.1101/2021.05.03.442406
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Functional characterisation of banana (Musa spp.) 2-oxoglutarate-dependent dioxygenases involved in flavonoid biosynthesis
Mareike Busche, Christopher Acatay, Stefan Martens, Bernd Weisshaar, Ralf Stracke
bioRxiv 2021.05.03.442406; doi: https://doi.org/10.1101/2021.05.03.442406

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