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Single-cell transcriptome analysis of the zebrafish embryonic trunk

View ORCID ProfileSanjeeva Metikala, View ORCID ProfileSatish Casie Chetty, View ORCID ProfileSaulius Sumanas
doi: https://doi.org/10.1101/2021.05.07.443129
Sanjeeva Metikala
1Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati OH 45229
2Department of Pathology and Cell Biology, USF Health Heart Institute, University of South Florida, Tampa, FL 33602
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  • ORCID record for Sanjeeva Metikala
Satish Casie Chetty
1Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati OH 45229
3Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati OH 45229
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  • For correspondence: ssumanas@usf.edu
Saulius Sumanas
1Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati OH 45229
2Department of Pathology and Cell Biology, USF Health Heart Institute, University of South Florida, Tampa, FL 33602
3Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati OH 45229
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  • For correspondence: ssumanas@usf.edu
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Abstract

During embryonic development, cells differentiate into a variety of distinct cell types and subtypes with diverse transcriptional profiles. To date, transcriptomic signatures of different cell lineages that arise during development have been only partially characterized. Here we used single-cell RNA-seq to perform transcriptomic analysis of over 20,000 cells disaggregated from the trunk region of zebrafish embryos at the 30 hpf stage. Transcriptional signatures of 27 different cell types and subtypes were identified and annotated during this analysis. This dataset will be a useful resource for many researchers in the fields of developmental and cellular biology and facilitate the understanding of molecular mechanisms that regulate cell lineage choices during development.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE152982

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted May 08, 2021.
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Single-cell transcriptome analysis of the zebrafish embryonic trunk
Sanjeeva Metikala, Satish Casie Chetty, Saulius Sumanas
bioRxiv 2021.05.07.443129; doi: https://doi.org/10.1101/2021.05.07.443129
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Single-cell transcriptome analysis of the zebrafish embryonic trunk
Sanjeeva Metikala, Satish Casie Chetty, Saulius Sumanas
bioRxiv 2021.05.07.443129; doi: https://doi.org/10.1101/2021.05.07.443129

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