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SCON - A Short Conditional intrON for conditional knockout with one-step zygote injection

View ORCID ProfileSzu-Hsien Sam Wu, Réka Szép-Bakonyi, View ORCID ProfileHeetak Lee, View ORCID ProfileGabriele Colozza, Ayse Boese, View ORCID ProfileKrista Rene Gert, Natalia Hallay, Jihoon Kim, Yi Zhu, Sandra Pilat-Carotta, View ORCID ProfileHans-Christian Theussl, View ORCID ProfileAndrea Pauli, View ORCID ProfileBon-Kyoung Koo
doi: https://doi.org/10.1101/2021.05.09.443220
Szu-Hsien Sam Wu
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
2Vienna BioCenter PhD Program, Doctoral School of the University at Vienna and Medical University of Vienna
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  • ORCID record for Szu-Hsien Sam Wu
Réka Szép-Bakonyi
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
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Heetak Lee
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
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Gabriele Colozza
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
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Ayse Boese
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
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Krista Rene Gert
2Vienna BioCenter PhD Program, Doctoral School of the University at Vienna and Medical University of Vienna
3Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Campus-Vienna-BioCenter 1, 1030 Vienna, Austria
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Natalia Hallay
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
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Jihoon Kim
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
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Yi Zhu
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
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Sandra Pilat-Carotta
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
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Hans-Christian Theussl
4IMP/IMBA Transgenic Service, Institute of Molecular Pathology (IMP), Vienna, Austria
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Andrea Pauli
3Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Campus-Vienna-BioCenter 1, 1030 Vienna, Austria
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Bon-Kyoung Koo
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
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  • For correspondence: bonkyoung.koo@imba.oeaw.ac.at
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Abstract

The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables rapid generation of conditional alleles via one-step zygote injection. SCON has conditional intronic function in various vertebrate species and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted May 10, 2021.
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SCON - A Short Conditional intrON for conditional knockout with one-step zygote injection
Szu-Hsien Sam Wu, Réka Szép-Bakonyi, Heetak Lee, Gabriele Colozza, Ayse Boese, Krista Rene Gert, Natalia Hallay, Jihoon Kim, Yi Zhu, Sandra Pilat-Carotta, Hans-Christian Theussl, Andrea Pauli, Bon-Kyoung Koo
bioRxiv 2021.05.09.443220; doi: https://doi.org/10.1101/2021.05.09.443220
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SCON - A Short Conditional intrON for conditional knockout with one-step zygote injection
Szu-Hsien Sam Wu, Réka Szép-Bakonyi, Heetak Lee, Gabriele Colozza, Ayse Boese, Krista Rene Gert, Natalia Hallay, Jihoon Kim, Yi Zhu, Sandra Pilat-Carotta, Hans-Christian Theussl, Andrea Pauli, Bon-Kyoung Koo
bioRxiv 2021.05.09.443220; doi: https://doi.org/10.1101/2021.05.09.443220

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