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Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research

Gil Lola Oreff, Michele Fenu, Claus Vogl, Iris Ribitsch, View ORCID ProfileFlorien Jenner
doi: https://doi.org/10.1101/2021.05.09.443263
Gil Lola Oreff
1University of Veterinary Medicine Vienna, Department of Companion Animals and Horses, Equine Surgery Unit, VETERM, Veterinaerplatz 1, 1210 Vienna, Austria
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Michele Fenu
1University of Veterinary Medicine Vienna, Department of Companion Animals and Horses, Equine Surgery Unit, VETERM, Veterinaerplatz 1, 1210 Vienna, Austria
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Claus Vogl
2University of Veterinary Medicine Vienna, Department of Biomedical Sciences, Institute of Animal Breeding and Genetics, Veterinaerplatz 1, 1210 Vienna, Austria
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Iris Ribitsch
1University of Veterinary Medicine Vienna, Department of Companion Animals and Horses, Equine Surgery Unit, VETERM, Veterinaerplatz 1, 1210 Vienna, Austria
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Florien Jenner
1University of Veterinary Medicine Vienna, Department of Companion Animals and Horses, Equine Surgery Unit, VETERM, Veterinaerplatz 1, 1210 Vienna, Austria
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  • ORCID record for Florien Jenner
  • For correspondence: Florien.Jenner@vetmeduni.ac.at
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Abstract

For research on tendon injury, many different animal models are utilized; however, the extent to which these species simulate the clinical condition and disease pathophysiology has not yet been critically evaluated. Considering the importance of inflammation in tendon disease, this study compared the cellular and molecular features of inflammation in tenocytes of humans and four common model species (mouse, rat, sheep, and horse). While mouse and rat tenocytes most closely equalled human tenocytes’ low proliferation capacity and the negligible effect of inflammation on proliferation, the wound closure speed of humans was best approximated by rats and horses. The overall gene expression of human tenocytes was most similar to mice under healthy, to horses under transient and to sheep under constant inflammatory conditions. Humans were best matched by mice and horses in their tendon marker and collagen expression, by horses in extracellular matrix remodelling genes, and by rats in inflammatory mediators. As no single animal model perfectly replicates the clinical condition and sufficiently emulates human tenocytes, fit-for-purpose selection of the model species for each specific research question and combination of data from multiple species will be essential to optimize translational predictive validity.

Competing Interest Statement

The authors have declared no competing interest.

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Posted May 10, 2021.
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Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research
Gil Lola Oreff, Michele Fenu, Claus Vogl, Iris Ribitsch, Florien Jenner
bioRxiv 2021.05.09.443263; doi: https://doi.org/10.1101/2021.05.09.443263
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Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research
Gil Lola Oreff, Michele Fenu, Claus Vogl, Iris Ribitsch, Florien Jenner
bioRxiv 2021.05.09.443263; doi: https://doi.org/10.1101/2021.05.09.443263

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