Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Wiring logic of the early rodent olfactory system revealed by high-throughput sequencing of single neuron projections

Yushu Chen, View ORCID ProfileXiaoyin Chen, View ORCID ProfileBatuhan Baserdem, View ORCID ProfileHuiqing Zhan, Yan Li, Martin B. Davis, View ORCID ProfileJustus M. Kebschull, View ORCID ProfileAnthony M. Zador, Alexei A. Koulakov, View ORCID ProfileDinu F. Albeanu
doi: https://doi.org/10.1101/2021.05.12.443929
Yushu Chen
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiaoyin Chen
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Xiaoyin Chen
Batuhan Baserdem
2Stony Brook University, Stony Brook, NY
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Batuhan Baserdem
Huiqing Zhan
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Huiqing Zhan
Yan Li
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Martin B. Davis
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Justus M. Kebschull
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724
3Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Justus M. Kebschull
Anthony M. Zador
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Anthony M. Zador
  • For correspondence: albeanu@cshl.edu koulakov@cshl.edu zador@cshl.edu
Alexei A. Koulakov
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: albeanu@cshl.edu koulakov@cshl.edu zador@cshl.edu
Dinu F. Albeanu
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Dinu F. Albeanu
  • For correspondence: albeanu@cshl.edu koulakov@cshl.edu zador@cshl.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Data/Code
  • Preview PDF
Loading

Abstract

The structure of neuronal connectivity often provides insights into the relevant stimulus features, such as spatial location, orientation, sound frequency, etc1–6. The olfactory system, however, appears to lack structured connectivity as suggested by reports of broad and distributed connections both from the olfactory bulb to the piriform cortex7–22 and within the cortex23–25. These studies have inspired computational models of circuit function that rely on random connectivity26–33. It remains, nonetheless, unclear whether the olfactory connectivity contains spatial structure. Here, we use high throughput anatomical methods (MAPseq and BARseq)34–38 to analyze the projections of 5,309 bulb and 30,433 piriform cortex output neurons in the mouse at single-cell resolution. We identify previously unrecognized spatial organization in connectivity along the anterior-posterior axis (A-P) of the piriform cortex. We find that both the bulb projections to the cortex and the cortical outputs are not random, but rather form gradients along the A-P axis. Strikingly, these gradients are matched: bulb neurons targeting a given location within the piriform cortex co-innervate extra-piriform regions that receive strong inputs from neurons within that piriform locus. We also identify signatures of local connectivity in the piriform cortex. Our findings suggest an organizing principle of matched direct and indirect olfactory pathways that innervate extra-piriform targets in a coordinated manner, thus supporting models of information processing that rely on structured connectivity within the olfactory system.

Competing Interest Statement

A.M.Z. is a founder and equity owner of Cajal Neuroscience and a member of its scientific advisory board. The remaining authors declare no competing interests.

Footnotes

  • https://data.mendeley.com/datasets/6t9mb3yydy/draft?a=8a33a230-3176-40ca-9ad9-a8727afa31b6

  • https://github.com/KoulakovLab/OlfactoryProjectomeAnalysis

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted May 14, 2021.
Download PDF

Supplementary Material

Data/Code
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Wiring logic of the early rodent olfactory system revealed by high-throughput sequencing of single neuron projections
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Wiring logic of the early rodent olfactory system revealed by high-throughput sequencing of single neuron projections
Yushu Chen, Xiaoyin Chen, Batuhan Baserdem, Huiqing Zhan, Yan Li, Martin B. Davis, Justus M. Kebschull, Anthony M. Zador, Alexei A. Koulakov, Dinu F. Albeanu
bioRxiv 2021.05.12.443929; doi: https://doi.org/10.1101/2021.05.12.443929
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Wiring logic of the early rodent olfactory system revealed by high-throughput sequencing of single neuron projections
Yushu Chen, Xiaoyin Chen, Batuhan Baserdem, Huiqing Zhan, Yan Li, Martin B. Davis, Justus M. Kebschull, Anthony M. Zador, Alexei A. Koulakov, Dinu F. Albeanu
bioRxiv 2021.05.12.443929; doi: https://doi.org/10.1101/2021.05.12.443929

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4655)
  • Biochemistry (10308)
  • Bioengineering (7629)
  • Bioinformatics (26208)
  • Biophysics (13454)
  • Cancer Biology (10631)
  • Cell Biology (15354)
  • Clinical Trials (138)
  • Developmental Biology (8458)
  • Ecology (12761)
  • Epidemiology (2067)
  • Evolutionary Biology (16777)
  • Genetics (11361)
  • Genomics (15411)
  • Immunology (10557)
  • Microbiology (25063)
  • Molecular Biology (10163)
  • Neuroscience (54132)
  • Paleontology (398)
  • Pathology (1656)
  • Pharmacology and Toxicology (2878)
  • Physiology (4318)
  • Plant Biology (9206)
  • Scientific Communication and Education (1582)
  • Synthetic Biology (2543)
  • Systems Biology (6757)
  • Zoology (1453)