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STING mediates immune responses in a unicellular choanoflagellate

View ORCID ProfileArielle Woznica, Ashwani Kumar, View ORCID ProfileCarolyn R. Sturge, View ORCID ProfileChao Xing, View ORCID ProfileNicole King, View ORCID ProfileJulie K. Pfeiffer
doi: https://doi.org/10.1101/2021.05.13.443778
Arielle Woznica
1Department of Microbiology, University of Texas Southwestern Medical Center, Dallas TX 75390, USA
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  • For correspondence: Arielle.Woznica@UTSouthwestern.edu Julie.Pfeiffer@UTSouthwestern.edu
Ashwani Kumar
2McDermott Center Bioinformatics Lab, University of Texas Southwestern Medical Center, Dallas TX, USA
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Carolyn R. Sturge
1Department of Microbiology, University of Texas Southwestern Medical Center, Dallas TX 75390, USA
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Chao Xing
2McDermott Center Bioinformatics Lab, University of Texas Southwestern Medical Center, Dallas TX, USA
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Nicole King
3Howard Hughes Medical Institute, and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
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Julie K. Pfeiffer
1Department of Microbiology, University of Texas Southwestern Medical Center, Dallas TX 75390, USA
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  • ORCID record for Julie K. Pfeiffer
  • For correspondence: Arielle.Woznica@UTSouthwestern.edu Julie.Pfeiffer@UTSouthwestern.edu
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Abstract

Animals have evolved unique repertoires of innate immune genes and pathways that provide their first line of defense against pathogens. To reconstruct the ancestry of animal innate immunity, we have developed the choanoflagellate Monosiga brevicollis, one of the closest living relatives of animals, as a model for studying mechanisms underlying pathogen recognition and immune response. We found that M. brevicollis is killed by exposure to Pseudomonas aeruginosa bacteria and selectively avoids ingesting them. Moreover, M. brevicollis expresses STING, which, in animals, activates innate immune pathways in response to cyclic dinucleotides during pathogen sensing. M. brevicollis STING increases the susceptibility of M. brevicollis to P. aeruginosa-induced cell death and is required for responding to the cyclic dinucleotide 2’3’ cGAMP. Furthermore, similar to animals, autophagic signaling in M. brevicollis is induced by 2’3’ cGAMP in a STING-dependent manner. This study provides evidence for a pre-animal role for STING in antibacterial immunity and establishes M. brevicollis as a model system for the study of immune responses.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted May 14, 2021.
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STING mediates immune responses in a unicellular choanoflagellate
Arielle Woznica, Ashwani Kumar, Carolyn R. Sturge, Chao Xing, Nicole King, Julie K. Pfeiffer
bioRxiv 2021.05.13.443778; doi: https://doi.org/10.1101/2021.05.13.443778
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STING mediates immune responses in a unicellular choanoflagellate
Arielle Woznica, Ashwani Kumar, Carolyn R. Sturge, Chao Xing, Nicole King, Julie K. Pfeiffer
bioRxiv 2021.05.13.443778; doi: https://doi.org/10.1101/2021.05.13.443778

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