ABSTRACT
Calcium imaging using genetically encoded indicators (GECIs) is a widely adopted method to measure neural activity in modern neuroscience. Here, we explore the use of systemically administered viral vectors for brain-wide expression of GECIs, and adapt novel GECIs to optimize signal-to-noise. We show that systemic injections of PHP.eB AAVs to express GECIs is a highly promising technique for imaging neural activity and circumvent the need for transgenic GECI expressing mouse lines. We also establish the use of novel soma-targeted GECIs that outperform current Ca2+ indicators using both systemic and local virus injections.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
↵# shared senior authors
Copyright
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