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Selection-free non-viral method revealed highly efficient CRISPR-Cas9 genome editing of human pluripotent stem cells guided by cellular autophagy
Michelle Surma, Kavitha Anbarasu, Arupratan Das
doi: https://doi.org/10.1101/2021.05.16.444342
Michelle Surma
1Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University, Indianapolis, IN 46202, USA
Kavitha Anbarasu
1Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University, Indianapolis, IN 46202, USA
2Department of Medical and Molecular Genetics, Indiana University, Indianapolis, IN 46202, USA
Arupratan Das
1Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University, Indianapolis, IN 46202, USA
2Department of Medical and Molecular Genetics, Indiana University, Indianapolis, IN 46202, USA
3Stark Neurosciences Research Institute, Indiana University, Indianapolis, IN 46202, USA
4Department of Biochemistry and Molecular Biology, Indiana University, Indianapolis, IN 46202, USA
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Posted May 17, 2021.
Selection-free non-viral method revealed highly efficient CRISPR-Cas9 genome editing of human pluripotent stem cells guided by cellular autophagy
Michelle Surma, Kavitha Anbarasu, Arupratan Das
bioRxiv 2021.05.16.444342; doi: https://doi.org/10.1101/2021.05.16.444342
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