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The microbiome of Lotus nodules varies with plant health in a species-specific manner

View ORCID ProfileDuncan B. Crosbie, Maryam Mahmoudi, View ORCID ProfileViviane Radl, View ORCID ProfileAndreas Brachmann, View ORCID ProfileMichael Schloter, View ORCID ProfileEric Kemen, View ORCID ProfileMacarena Marín
doi: https://doi.org/10.1101/2021.05.19.441130
Duncan B. Crosbie
1Genetics, Biocentre, LMU Munich, Martinsried, Germany
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Maryam Mahmoudi
2Microbial Interactions in Plant Ecosystems, Centre for Plant Molecular Biology, University of Tübingen, Tübingen, Germany
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Viviane Radl
3Comparative Microbiome Analysis, Helmholtz Centre for Environmental Health, Munich, Germany
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Andreas Brachmann
1Genetics, Biocentre, LMU Munich, Martinsried, Germany
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Michael Schloter
3Comparative Microbiome Analysis, Helmholtz Centre for Environmental Health, Munich, Germany
4Chair for Soil Science, Technical University of Munich, Freising, Germany
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Eric Kemen
2Microbial Interactions in Plant Ecosystems, Centre for Plant Molecular Biology, University of Tübingen, Tübingen, Germany
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Macarena Marín
1Genetics, Biocentre, LMU Munich, Martinsried, Germany
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  • ORCID record for Macarena Marín
  • For correspondence: m.marin@bio.lmu.de
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ABSTRACT

Nitrogen fixation is carried out inside nodules of legumes by symbiotic rhizobia. Rhizobia dominate the nodule microbiome, however other non-rhizobial bacteria also colonise root nodules. It is not clear whether these less abundant nodule colonisers impact nodule function. In order to investigate the relationship between the nodule microbiome and nodule function as influenced by the soil microbiome, we used a metabarcoding approach to characterise the communities inside Lotus burttii, Lotus japonicus and Lotus corniculatus nodules from plants that were either starved or healthy resulting from inoculations with different soil suspensions in a closed pot experiment. We found that the nodule microbiome of all tested Lotus species differed according to inoculum, but only that of L. burttii varied with plant health. Using a machine learning algorithm, we also found that among the many non-rhizobial bacteria inside the nodule, amplicon sequence variants that were related to Pseudomonas were the most indicative signatures of a healthy plant nodule microbiome. These results support the hypothesis that legume nodule endophytes may play a role in the overall success of root-nodule symbiosis, albeit in a plant host specific manner.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted May 19, 2021.
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The microbiome of Lotus nodules varies with plant health in a species-specific manner
Duncan B. Crosbie, Maryam Mahmoudi, Viviane Radl, Andreas Brachmann, Michael Schloter, Eric Kemen, Macarena Marín
bioRxiv 2021.05.19.441130; doi: https://doi.org/10.1101/2021.05.19.441130
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The microbiome of Lotus nodules varies with plant health in a species-specific manner
Duncan B. Crosbie, Maryam Mahmoudi, Viviane Radl, Andreas Brachmann, Michael Schloter, Eric Kemen, Macarena Marín
bioRxiv 2021.05.19.441130; doi: https://doi.org/10.1101/2021.05.19.441130

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