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Reduced sensitivity of infectious SARS-CoV-2 variant B.1.617.2 to monoclonal antibodies and sera from convalescent and vaccinated individuals

View ORCID ProfileDelphine Planas, David Veyer, Artem Baidaliuk, Isabelle Staropoli, Florence Guivel-Benhassine, Maaran Michael Rajah, Cyril Planchais, Françoise Porrot, Nicolas Robillard, Julien Puech, Matthieu Prot, Floriane Gallais, Pierre Gantner, Aurélie Velay, Julien Le Guen, Najibi Kassis-Chikhani, Dhiaeddine Edriss, Laurent Belec, Aymeric Seve, Hélène Péré, Laura Courtellemont, Laurent Hocqueloux, Samira Fafi-Kremer, Thierry Prazuck, Hugo Mouquet, View ORCID ProfileTimothée Bruel, View ORCID ProfileEtienne Simon-Lorière, View ORCID ProfileFelix A. Rey, View ORCID ProfileOlivier Schwartz
doi: https://doi.org/10.1101/2021.05.26.445838
Delphine Planas
1Virus & Immunity Unit, Department of Virology, Institut Pasteur; CNRS UMR 3569, Paris, France
2Vaccine Research Institute, Creteil, France
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  • ORCID record for Delphine Planas
David Veyer
3INSERM, Functional Genomics of Solid Tumors (FunGeST), Centre de Recherche des Cordeliers, Université de Paris and Sorbonne Université, Paris, France
4Hôpital Européen Georges Pompidou, Laboratoire de Virologie, Service de Microbiologie, Paris, France
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Artem Baidaliuk
5G5 Evolutionary genomics of RNA viruses, Department of Virology, Institut Pasteur, Paris, France
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Isabelle Staropoli
1Virus & Immunity Unit, Department of Virology, Institut Pasteur; CNRS UMR 3569, Paris, France
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Florence Guivel-Benhassine
1Virus & Immunity Unit, Department of Virology, Institut Pasteur; CNRS UMR 3569, Paris, France
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Maaran Michael Rajah
1Virus & Immunity Unit, Department of Virology, Institut Pasteur; CNRS UMR 3569, Paris, France
6Université de Paris, Sorbonne Paris Cité, Paris, France
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Cyril Planchais
7Laboratory of Humoral Immunology, Department of Immunology, Institut Pasteur, INSERM U1222, Paris, France
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Françoise Porrot
1Virus & Immunity Unit, Department of Virology, Institut Pasteur; CNRS UMR 3569, Paris, France
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Nicolas Robillard
4Hôpital Européen Georges Pompidou, Laboratoire de Virologie, Service de Microbiologie, Paris, France
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Julien Puech
4Hôpital Européen Georges Pompidou, Laboratoire de Virologie, Service de Microbiologie, Paris, France
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Matthieu Prot
5G5 Evolutionary genomics of RNA viruses, Department of Virology, Institut Pasteur, Paris, France
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Floriane Gallais
8CHU de Strasbourg, Laboratoire de Virologie, Strasbourg, France
9Université de Strasbourg, INSERM, IRM UMR_S 1109, Strasbourg, France
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Pierre Gantner
8CHU de Strasbourg, Laboratoire de Virologie, Strasbourg, France
9Université de Strasbourg, INSERM, IRM UMR_S 1109, Strasbourg, France
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Aurélie Velay
8CHU de Strasbourg, Laboratoire de Virologie, Strasbourg, France
9Université de Strasbourg, INSERM, IRM UMR_S 1109, Strasbourg, France
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Julien Le Guen
10Hôpital Européen Georges Pompidou, Service de Gériatrie, Assistance Publique des Hôpitaux de Paris, Paris, France
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Najibi Kassis-Chikhani
11Hôpital européen Georges Pompidou, Unité d’Hygiène Hospitalière, Service de Microbiologie, Assistance Publique-Hôpitaux de Paris, Paris, 75015, France
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Dhiaeddine Edriss
4Hôpital Européen Georges Pompidou, Laboratoire de Virologie, Service de Microbiologie, Paris, France
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Laurent Belec
4Hôpital Européen Georges Pompidou, Laboratoire de Virologie, Service de Microbiologie, Paris, France
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Aymeric Seve
12CHR d’Orléans, service de maladies infectieuses, Orléans, France
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Hélène Péré
3INSERM, Functional Genomics of Solid Tumors (FunGeST), Centre de Recherche des Cordeliers, Université de Paris and Sorbonne Université, Paris, France
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Laura Courtellemont
12CHR d’Orléans, service de maladies infectieuses, Orléans, France
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Laurent Hocqueloux
12CHR d’Orléans, service de maladies infectieuses, Orléans, France
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Samira Fafi-Kremer
8CHU de Strasbourg, Laboratoire de Virologie, Strasbourg, France
9Université de Strasbourg, INSERM, IRM UMR_S 1109, Strasbourg, France
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Thierry Prazuck
12CHR d’Orléans, service de maladies infectieuses, Orléans, France
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Hugo Mouquet
7Laboratory of Humoral Immunology, Department of Immunology, Institut Pasteur, INSERM U1222, Paris, France
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Timothée Bruel
1Virus & Immunity Unit, Department of Virology, Institut Pasteur; CNRS UMR 3569, Paris, France
2Vaccine Research Institute, Creteil, France
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  • For correspondence: timothee.bruel@pasteur.fr olivier.schwartz@pasteur.fr
Etienne Simon-Lorière
5G5 Evolutionary genomics of RNA viruses, Department of Virology, Institut Pasteur, Paris, France
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Felix A. Rey
13Structural Virology Unit, Department of Virology, Institut Pasteur; CNRS UMR 3569, Paris, France
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Olivier Schwartz
1Virus & Immunity Unit, Department of Virology, Institut Pasteur; CNRS UMR 3569, Paris, France
2Vaccine Research Institute, Creteil, France
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  • ORCID record for Olivier Schwartz
  • For correspondence: timothee.bruel@pasteur.fr olivier.schwartz@pasteur.fr
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Abstract

The SARS-CoV-2 B.1.617 lineage emerged in October 2020 in India1–6. It has since then become dominant in some indian regions and further spread to many countries. The lineage includes three main subtypes (B1.617.1, B.1617.2 and B.1.617.3), which harbour diverse Spike mutations in the N-terminal domain (NTD) and the receptor binding domain (RBD) which may increase their immune evasion potential. B.1.617.2 is believed to spread faster than the other versions. Here, we isolated infectious B.1.617.2 from a traveller returning from India. We examined its sensitivity to monoclonal antibodies (mAbs) and to antibodies present in sera from COVID-19 convalescent individuals or vaccine recipients, in comparison to other viral lineages. B.1.617.2 was resistant to neutralization by some anti-NTD and anti-RBD mAbs, including Bamlanivimab, which were impaired in binding to the B.1.617.2 Spike. Sera from convalescent patients collected up to 12 months post symptoms and from Pfizer Comirnaty vaccine recipients were 3 to 6 fold less potent against B.1.617.2, relative to B.1.1.7. Sera from individuals having received one dose of AstraZeneca Vaxzevria barely inhibited B.1.617.2. Thus, B.1.617.2 spread is associated with an escape to antibodies targeting non-RBD and RBD Spike epitopes.

Competing Interest Statement

C.P., H.M., O.S, T.B., F.R. have a pending patent application for the anti-RBD mAbs described in the present study (PCT/FR2021/070522).

Footnotes

  • ↵** co-last authors

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Reduced sensitivity of infectious SARS-CoV-2 variant B.1.617.2 to monoclonal antibodies and sera from convalescent and vaccinated individuals
Delphine Planas, David Veyer, Artem Baidaliuk, Isabelle Staropoli, Florence Guivel-Benhassine, Maaran Michael Rajah, Cyril Planchais, Françoise Porrot, Nicolas Robillard, Julien Puech, Matthieu Prot, Floriane Gallais, Pierre Gantner, Aurélie Velay, Julien Le Guen, Najibi Kassis-Chikhani, Dhiaeddine Edriss, Laurent Belec, Aymeric Seve, Hélène Péré, Laura Courtellemont, Laurent Hocqueloux, Samira Fafi-Kremer, Thierry Prazuck, Hugo Mouquet, Timothée Bruel, Etienne Simon-Lorière, Felix A. Rey, Olivier Schwartz
bioRxiv 2021.05.26.445838; doi: https://doi.org/10.1101/2021.05.26.445838
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Reduced sensitivity of infectious SARS-CoV-2 variant B.1.617.2 to monoclonal antibodies and sera from convalescent and vaccinated individuals
Delphine Planas, David Veyer, Artem Baidaliuk, Isabelle Staropoli, Florence Guivel-Benhassine, Maaran Michael Rajah, Cyril Planchais, Françoise Porrot, Nicolas Robillard, Julien Puech, Matthieu Prot, Floriane Gallais, Pierre Gantner, Aurélie Velay, Julien Le Guen, Najibi Kassis-Chikhani, Dhiaeddine Edriss, Laurent Belec, Aymeric Seve, Hélène Péré, Laura Courtellemont, Laurent Hocqueloux, Samira Fafi-Kremer, Thierry Prazuck, Hugo Mouquet, Timothée Bruel, Etienne Simon-Lorière, Felix A. Rey, Olivier Schwartz
bioRxiv 2021.05.26.445838; doi: https://doi.org/10.1101/2021.05.26.445838

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