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Crossing design shapes patterns of genetic variation in synthetic recombinant populations of Saccharomyces cerevisiae

View ORCID ProfileMark A. Phillips, Ian C. Kutch, Kaitlin M. McHugh, Savannah K. Taggard, View ORCID ProfileMolly K. Burke
doi: https://doi.org/10.1101/2021.05.26.445861
Mark A. Phillips
1Department of Integrative Biology, Oregon State University, Corvallis, OR 97331
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  • For correspondence: philmark@oregonstate.edu molly.burke@oregonstate.edu
Ian C. Kutch
1Department of Integrative Biology, Oregon State University, Corvallis, OR 97331
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Kaitlin M. McHugh
1Department of Integrative Biology, Oregon State University, Corvallis, OR 97331
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Savannah K. Taggard
1Department of Integrative Biology, Oregon State University, Corvallis, OR 97331
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Molly K. Burke
1Department of Integrative Biology, Oregon State University, Corvallis, OR 97331
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  • ORCID record for Molly K. Burke
  • For correspondence: philmark@oregonstate.edu molly.burke@oregonstate.edu
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Abstract

“Synthetic recombinant” populations have emerged as a useful tool for dissecting the genetics of complex traits. They can be used to derive inbred lines for fine QTL mapping, or the populations themselves can be sampled for experimental evolution. In latter application, investigators generally value maximizing genetic variation in constructed populations. This is because in evolution experiments initiated from such populations, adaptation is primarily fueled by standing genetic variation. Despite this reality, little has been done to systematically evaluate how different methods of constructing synthetic populations shape initial patterns of variation. Here we seek to address this issue by comparing outcomes in synthetic recombinant Saccharomyces cerevisiae populations created using one of two strategies: pairwise crossing of isogenic strains or simple mixing of strains in equal proportion. We also explore the impact of the varying the number of parental strains. We find that more genetic variation is initially present and maintained when population construction includes a round of pairwise crossing. As perhaps expected, we also observe that increasing the number of parental strains typically increases genetic diversity. In summary, we suggest that when constructing populations for use in evolution experiments, simply mixing founder strains in equal proportion may limit the adaptive potential.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 01, 2021.
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Crossing design shapes patterns of genetic variation in synthetic recombinant populations of Saccharomyces cerevisiae
Mark A. Phillips, Ian C. Kutch, Kaitlin M. McHugh, Savannah K. Taggard, Molly K. Burke
bioRxiv 2021.05.26.445861; doi: https://doi.org/10.1101/2021.05.26.445861
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Crossing design shapes patterns of genetic variation in synthetic recombinant populations of Saccharomyces cerevisiae
Mark A. Phillips, Ian C. Kutch, Kaitlin M. McHugh, Savannah K. Taggard, Molly K. Burke
bioRxiv 2021.05.26.445861; doi: https://doi.org/10.1101/2021.05.26.445861

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