Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Oligodendrocyte precursor cells prune axons in the mouse neocortex

View ORCID ProfileJoAnn Buchanan, Leila Elabbady, View ORCID ProfileForrest Collman, View ORCID ProfileNikolas L. Jorstad, View ORCID ProfileTrygve E. Bakken, View ORCID ProfileCarolyn Ott, View ORCID ProfileJenna Glatzer, View ORCID ProfileAdam A. Bleckert, View ORCID ProfileAgnes L. Bodor, Derrick Brittan, Daniel J. Bumbarger, View ORCID ProfileGayathri Mahalingam, View ORCID ProfileSharmishtaa Seshamani, View ORCID ProfileCasey Schneider-Mizell, View ORCID ProfileMarc M. Takeno, View ORCID ProfileRussel Torres, View ORCID ProfileWenjing Yin, View ORCID ProfileRebecca D. Hodge, Manuel Castro, View ORCID ProfileSven Dorkenwald, Dodam Ih, View ORCID ProfileChris S. Jordan, View ORCID ProfileNico Kemnitz, Kisuk Lee, Ran Lu, View ORCID ProfileThomas Macrina, Shang Mu, View ORCID ProfileSergiy Popovych, William M. Silversmith, View ORCID ProfileIgnacio Tartavull, View ORCID ProfileNicholas L. Turner, Alyssa M. Wilson, William Wong, View ORCID ProfileJingpeng Wu, View ORCID ProfileAleksandar Zlateski, View ORCID ProfileJonathan Zung, Jennifer Lippincott-Schwartz, View ORCID ProfileEd S. Lein, H. Sebastian Seung, View ORCID ProfileDwight E. Bergles, View ORCID ProfileR. Clay Reid, View ORCID ProfileNuno Maçarico da Costa
doi: https://doi.org/10.1101/2021.05.29.446047
JoAnn Buchanan
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for JoAnn Buchanan
  • For correspondence: joannb@alleninstitute.org
Leila Elabbady
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Forrest Collman
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Forrest Collman
Nikolas L. Jorstad
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Nikolas L. Jorstad
Trygve E. Bakken
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Trygve E. Bakken
Carolyn Ott
2Janelia Research Campus, Howard Hughes Medical Institute
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Carolyn Ott
Jenna Glatzer
3The Solomon H. Snyder Department of Neuroscience, John Hopkins University School of Medicine
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jenna Glatzer
Adam A. Bleckert
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Adam A. Bleckert
Agnes L. Bodor
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Agnes L. Bodor
Derrick Brittan
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel J. Bumbarger
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gayathri Mahalingam
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Gayathri Mahalingam
Sharmishtaa Seshamani
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sharmishtaa Seshamani
Casey Schneider-Mizell
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Casey Schneider-Mizell
Marc M. Takeno
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Marc M. Takeno
Russel Torres
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Russel Torres
Wenjing Yin
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Wenjing Yin
Rebecca D. Hodge
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Rebecca D. Hodge
Manuel Castro
4Princeton Neuroscience Institute, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sven Dorkenwald
4Princeton Neuroscience Institute, Princeton University
5Computer Science Department, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sven Dorkenwald
Dodam Ih
4Princeton Neuroscience Institute, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chris S. Jordan
4Princeton Neuroscience Institute, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Chris S. Jordan
Nico Kemnitz
4Princeton Neuroscience Institute, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Nico Kemnitz
Kisuk Lee
4Princeton Neuroscience Institute, Princeton University
5Computer Science Department, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ran Lu
4Princeton Neuroscience Institute, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas Macrina
4Princeton Neuroscience Institute, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Thomas Macrina
Shang Mu
4Princeton Neuroscience Institute, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sergiy Popovych
5Computer Science Department, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sergiy Popovych
William M. Silversmith
5Computer Science Department, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ignacio Tartavull
5Computer Science Department, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ignacio Tartavull
Nicholas L. Turner
4Princeton Neuroscience Institute, Princeton University
5Computer Science Department, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Nicholas L. Turner
Alyssa M. Wilson
4Princeton Neuroscience Institute, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
William Wong
4Princeton Neuroscience Institute, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jingpeng Wu
4Princeton Neuroscience Institute, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jingpeng Wu
Aleksandar Zlateski
4Princeton Neuroscience Institute, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Aleksandar Zlateski
Jonathan Zung
4Princeton Neuroscience Institute, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jonathan Zung
Jennifer Lippincott-Schwartz
2Janelia Research Campus, Howard Hughes Medical Institute
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ed S. Lein
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ed S. Lein
H. Sebastian Seung
4Princeton Neuroscience Institute, Princeton University
5Computer Science Department, Princeton University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dwight E. Bergles
3The Solomon H. Snyder Department of Neuroscience, John Hopkins University School of Medicine
6Johns Hopkins Kavli Neuroscience Discovery Institute
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Dwight E. Bergles
R. Clay Reid
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for R. Clay Reid
Nuno Maçarico da Costa
1Allen Institute for Brain Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Nuno Maçarico da Costa
  • For correspondence: joannb@alleninstitute.org
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Data/Code
  • Preview PDF
Loading

ABSTRACT

Neurons in the developing brain undergo extensive structural refinement as nascent circuits adopt their mature form1. This transformation is facilitated by the engulfment and degradation of excess axonal branches and inappropriate synapses by surrounding glial cells, including microglia and astrocytes2,3. However, the small size of phagocytic organelles and the complex, highly ramified morphology of glia has made it difficult to determine the contribution of these and other glial cell types to this process. Here, we used large scale, serial electron microscopy (ssEM) with computational volume segmentation to reconstruct the complete 3D morphologies of distinct glial types in the mouse visual cortex. Unexpectedly, we discovered that the fine processes of oligodendrocyte precursor cells (OPCs), a population of abundant, highly dynamic glial progenitors4, frequently surrounded terminal axon branches and included numerous phagolysosomes (PLs) containing fragments of axons and presynaptic terminals. Single- nucleus RNA sequencing indicated that cortical OPCs express key phagocytic genes, as well as neuronal transcripts, consistent with active axonal engulfment. PLs were ten times more abundant in OPCs than in microglia in P36 mice, and declined with age and lineage progression, suggesting that OPCs contribute very substantially to refinement of neuronal circuits during later phases of cortical development.

Competing Interest Statement

TM and HSS disclose financial interests in Zetta AI LLC.

Footnotes

  • https://microns-explorer.org/

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted May 29, 2021.
Download PDF

Supplementary Material

Data/Code
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Oligodendrocyte precursor cells prune axons in the mouse neocortex
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Oligodendrocyte precursor cells prune axons in the mouse neocortex
JoAnn Buchanan, Leila Elabbady, Forrest Collman, Nikolas L. Jorstad, Trygve E. Bakken, Carolyn Ott, Jenna Glatzer, Adam A. Bleckert, Agnes L. Bodor, Derrick Brittan, Daniel J. Bumbarger, Gayathri Mahalingam, Sharmishtaa Seshamani, Casey Schneider-Mizell, Marc M. Takeno, Russel Torres, Wenjing Yin, Rebecca D. Hodge, Manuel Castro, Sven Dorkenwald, Dodam Ih, Chris S. Jordan, Nico Kemnitz, Kisuk Lee, Ran Lu, Thomas Macrina, Shang Mu, Sergiy Popovych, William M. Silversmith, Ignacio Tartavull, Nicholas L. Turner, Alyssa M. Wilson, William Wong, Jingpeng Wu, Aleksandar Zlateski, Jonathan Zung, Jennifer Lippincott-Schwartz, Ed S. Lein, H. Sebastian Seung, Dwight E. Bergles, R. Clay Reid, Nuno Maçarico da Costa
bioRxiv 2021.05.29.446047; doi: https://doi.org/10.1101/2021.05.29.446047
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Oligodendrocyte precursor cells prune axons in the mouse neocortex
JoAnn Buchanan, Leila Elabbady, Forrest Collman, Nikolas L. Jorstad, Trygve E. Bakken, Carolyn Ott, Jenna Glatzer, Adam A. Bleckert, Agnes L. Bodor, Derrick Brittan, Daniel J. Bumbarger, Gayathri Mahalingam, Sharmishtaa Seshamani, Casey Schneider-Mizell, Marc M. Takeno, Russel Torres, Wenjing Yin, Rebecca D. Hodge, Manuel Castro, Sven Dorkenwald, Dodam Ih, Chris S. Jordan, Nico Kemnitz, Kisuk Lee, Ran Lu, Thomas Macrina, Shang Mu, Sergiy Popovych, William M. Silversmith, Ignacio Tartavull, Nicholas L. Turner, Alyssa M. Wilson, William Wong, Jingpeng Wu, Aleksandar Zlateski, Jonathan Zung, Jennifer Lippincott-Schwartz, Ed S. Lein, H. Sebastian Seung, Dwight E. Bergles, R. Clay Reid, Nuno Maçarico da Costa
bioRxiv 2021.05.29.446047; doi: https://doi.org/10.1101/2021.05.29.446047

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4685)
  • Biochemistry (10362)
  • Bioengineering (7682)
  • Bioinformatics (26343)
  • Biophysics (13534)
  • Cancer Biology (10694)
  • Cell Biology (15446)
  • Clinical Trials (138)
  • Developmental Biology (8501)
  • Ecology (12824)
  • Epidemiology (2067)
  • Evolutionary Biology (16867)
  • Genetics (11402)
  • Genomics (15484)
  • Immunology (10621)
  • Microbiology (25226)
  • Molecular Biology (10225)
  • Neuroscience (54482)
  • Paleontology (402)
  • Pathology (1669)
  • Pharmacology and Toxicology (2897)
  • Physiology (4345)
  • Plant Biology (9254)
  • Scientific Communication and Education (1587)
  • Synthetic Biology (2558)
  • Systems Biology (6781)
  • Zoology (1466)