Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Complete protection by a single dose skin patch delivered SARS-CoV-2 spike vaccine

Christopher L.D. McMillan, Jovin J.Y. Choo, Adi Idris, Aroon Supramaniam, Naphak Modhiran, Alberto A. Amarilla, Ariel Isaacs, Stacey T.M. Cheung, Benjamin Liang, Helle Bielefeldt-Ohmann, Armira Azuar, Dhruba Acharya, Gabrielle Kelly, Germain J.P. Fernando, Michael J. Landsberg, Alexander A. Khromykh, Daniel Watterson, Paul R. Young, Nigel A.J. McMillan, View ORCID ProfileDavid A. Muller
doi: https://doi.org/10.1101/2021.05.30.446357
Christopher L.D. McMillan
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jovin J.Y. Choo
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Adi Idris
2Menzies Health Institute Queensland, School of Pharmacy, Anatomy and Medical Sciences, Griffith University; Gold Coast, Queensland 4222, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aroon Supramaniam
2Menzies Health Institute Queensland, School of Pharmacy, Anatomy and Medical Sciences, Griffith University; Gold Coast, Queensland 4222, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Naphak Modhiran
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alberto A. Amarilla
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ariel Isaacs
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stacey T.M. Cheung
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benjamin Liang
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Helle Bielefeldt-Ohmann
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
4Australian Infectious Diseases Research Centre, Global Virus Network Centre of Excellence; Brisbane, Queensland 4072 and 4029, Australia
5School of Veterinary Science, The University of Queensland Gatton Campus, Gatton, Queensland 4343, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Armira Azuar
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dhruba Acharya
2Menzies Health Institute Queensland, School of Pharmacy, Anatomy and Medical Sciences, Griffith University; Gold Coast, Queensland 4222, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gabrielle Kelly
2Menzies Health Institute Queensland, School of Pharmacy, Anatomy and Medical Sciences, Griffith University; Gold Coast, Queensland 4222, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Germain J.P. Fernando
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
3Vaxxas Pty Ltd, Translational Research Institute; 37 Kent Street, Brisbane, Queensland 4102, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael J. Landsberg
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
4Australian Infectious Diseases Research Centre, Global Virus Network Centre of Excellence; Brisbane, Queensland 4072 and 4029, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alexander A. Khromykh
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
4Australian Infectious Diseases Research Centre, Global Virus Network Centre of Excellence; Brisbane, Queensland 4072 and 4029, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel Watterson
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
4Australian Infectious Diseases Research Centre, Global Virus Network Centre of Excellence; Brisbane, Queensland 4072 and 4029, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paul R. Young
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
4Australian Infectious Diseases Research Centre, Global Virus Network Centre of Excellence; Brisbane, Queensland 4072 and 4029, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nigel A.J. McMillan
2Menzies Health Institute Queensland, School of Pharmacy, Anatomy and Medical Sciences, Griffith University; Gold Coast, Queensland 4222, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David A. Muller
1School of Chemistry and Molecular Biosciences, The University of Queensland; St Lucia, Queensland 4072, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for David A. Muller
  • For correspondence: d.muller4@uq.edu.au
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

SARS-CoV-2 has infected over 160 million people and resulted in more than 3.3 million deaths, and we still face many challenges in the rollout of vaccines. Here, we use the high-density microarray patch to deliver a SARS-CoV-2 spike subunit vaccine directly to the skin. We show the vaccine, dry-coated on the patch is thermostable, and delivery of spike via HD-MAP induced greater cellular and antibody immune responses, with serum able to potently neutralize clinically relevant isolates including those from the B.1.1.7 and B.1.351 lineages. Finally, a single dose of HD-MAP-delivered spike provided complete protection from a lethal virus challenge, demonstrating that HD-MAP delivery of a SARS-CoV-2 vaccine is superior to traditional needle-and-syringe vaccination and has the potential to greatly impact the ongoing COVID-19 pandemic.

Competing Interest Statement

GJPF, PRY and DAM are consultants of the HD-MAP development company Vaxxas Pty Ltd. The remaining authors declare that they have no competing interests.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted May 31, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Complete protection by a single dose skin patch delivered SARS-CoV-2 spike vaccine
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Complete protection by a single dose skin patch delivered SARS-CoV-2 spike vaccine
Christopher L.D. McMillan, Jovin J.Y. Choo, Adi Idris, Aroon Supramaniam, Naphak Modhiran, Alberto A. Amarilla, Ariel Isaacs, Stacey T.M. Cheung, Benjamin Liang, Helle Bielefeldt-Ohmann, Armira Azuar, Dhruba Acharya, Gabrielle Kelly, Germain J.P. Fernando, Michael J. Landsberg, Alexander A. Khromykh, Daniel Watterson, Paul R. Young, Nigel A.J. McMillan, David A. Muller
bioRxiv 2021.05.30.446357; doi: https://doi.org/10.1101/2021.05.30.446357
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Complete protection by a single dose skin patch delivered SARS-CoV-2 spike vaccine
Christopher L.D. McMillan, Jovin J.Y. Choo, Adi Idris, Aroon Supramaniam, Naphak Modhiran, Alberto A. Amarilla, Ariel Isaacs, Stacey T.M. Cheung, Benjamin Liang, Helle Bielefeldt-Ohmann, Armira Azuar, Dhruba Acharya, Gabrielle Kelly, Germain J.P. Fernando, Michael J. Landsberg, Alexander A. Khromykh, Daniel Watterson, Paul R. Young, Nigel A.J. McMillan, David A. Muller
bioRxiv 2021.05.30.446357; doi: https://doi.org/10.1101/2021.05.30.446357

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3609)
  • Biochemistry (7585)
  • Bioengineering (5533)
  • Bioinformatics (20825)
  • Biophysics (10344)
  • Cancer Biology (7995)
  • Cell Biology (11653)
  • Clinical Trials (138)
  • Developmental Biology (6617)
  • Ecology (10224)
  • Epidemiology (2065)
  • Evolutionary Biology (13639)
  • Genetics (9557)
  • Genomics (12856)
  • Immunology (7930)
  • Microbiology (19568)
  • Molecular Biology (7675)
  • Neuroscience (42182)
  • Paleontology (308)
  • Pathology (1259)
  • Pharmacology and Toxicology (2208)
  • Physiology (3271)
  • Plant Biology (7058)
  • Scientific Communication and Education (1295)
  • Synthetic Biology (1953)
  • Systems Biology (5433)
  • Zoology (1119)